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Cited by 485Clostridium difficile and Clostridium perfringens researchGut microbiota and healthGastrointestinal motility and disorders

Intestinal dysbiosis and depletion of butyrogenic bacteria in Clostridium difficile infection and nosocomial diarrhea.

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Antharam, Vijay C., Li, Eric C., Ishmael, Arif, Sharma, Anuj, Mai, Volker, Rand, Kenneth H., Wang, Gary P.·2013·Journal of Clinical Microbiology
DOI 10.1128/jcm.00845-13PMC PMC3754663
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Abstract

Clostridium difficile infection (CDI) causes nearly half a million cases of diarrhea and colitis in the United States each year. Although the importance of the gut microbiota in C. difficile pathogenesis is well recognized, components of the human gut flora critical for colonization resistance are not known. Culture-independent high-density Roche 454 pyrosequencing was used to survey the distal gut microbiota for 39 individuals with CDI, 36 subjects with C. difficile-negative nosocomial diarrhea (CDN), and 40 healthy control subjects. A total of 526,071 partial 16S rRNA sequence reads of the V1 to V3 regions were aligned with 16S databases, identifying 3,531 bacterial phylotypes from 115 fecal samples. Genomic analysis revealed significant alterations of organism lineages in both the CDI and CDN groups, which were accompanied by marked decreases in microbial diversity and species richness driven primarily by a paucity of phylotypes within the Firmicutes phylum. Normally abundant gut commensal organisms, including the Ruminococcaceae and Lachnospiraceae families and butyrate-producing C2 to C4 anaerobic fermenters, were significantly depleted in the CDI and CDN groups. These data demonstrate associations between the depletion of Ruminococcaceae, Lachnospiraceae, and butyrogenic bacteria in the gut microbiota and nosocomial diarrhea, including C. difficile infection. Mechanistic studies focusing on the functional roles of these organisms in diarrheal diseases and resistance against C. difficile colonization are warranted.

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  • In domestic or captive animal environments and in humans, the initiation of dysbiosis leading to domination by anaerobic clostridia, particularly by Clostridioides difficile, has been linked to antibiotic treatments, hospitalization, and neonatal microbiomes.

    “In domestic or captive animal environments and in humans, the initiation of dysbiosis leading to domination by anaerobic clostridia, particularly by Clostridioides difficile, has been linked to antibiotic treatments, hospitalization, and neonatal microbiomes (Antharam et al., 2013;Cassir et al., 2015;Popoff, 2018).”

    From Novel Lethal Clostridial Infection in Florida Manatees (Trichechus manatus latirostris): Cause of the 2013 Unusual Mortality Event in the Indian River Lagoon (2022) · cites this paper as Antharam et al., 2013

    The Introduction links antibiotic-induced disruption of the human gut microbiota to C. difficile proliferation, and the Discussion reports that antibiotic exposure during hospitalization was strongly associated with subsequent infection (pages unavailable). The paper does not establish this pattern in domestic or captive animal environments or link it to neonatal microbiomes. It reports depletion of several normally abundant anaerobic bacteria, not general domination by anaerobic clostridia. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Antharam, V. C., Li, E. C., Ishmael, A., Sharma, A., Mai, V., Rand, K. H., & Wang, G. P.
Affiliations
University of Florida (US)
ORCID
Venue
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Published
2013-09
Volume
51
Issue
9
Pages
2884-2892
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
DOI
10.1128/jcm.00845-13
PMC
PMC3754663
OpenAlex
W2096436144
Cited by
485
References
6
Citations by year
2026: 18 · 2025: 20 · 2024: 34 · 2023: 26 · 2022: 43 · 2021: 62 · 2020: 51 · 2019: 45 · 2018: 55 · 2017: 34 · 2016: 41 · 2015: 26 · 2014: 27 · 2013: 3
FWCI
23.59
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Clinical Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Florida
  • University College Cork
  • National Institutes of Health
  • National Institute of Allergy and Infectious Diseases (KO8 AI077713, K08AI077713)
  • National Center for Research Resources (UL1RR029890)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
Clostridium difficile infection, nosocomial diarrhea, gut microbiota, gut dysbiosis, Ruminococcaceae, Lachnospiraceae, Firmicutes, microbial diversity, colonization resistance, 16S rRNA gene sequencing
MeSH terms
Adult, Aged, Bacteria, Clostridium Infections, Cross Infection, Diarrhea, DNA, Bacterial, DNA, Ribosomal, Female, Humans, Male, Middle Aged, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, United States, Cluster Analysis, Sequence Analysis, DNA, Butyric Acid, Gastrointestinal Tract, Metagenomics, Biota, Dysbiosis

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