The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus
Open accessAbstract
The development and evaluation of a one-step single-tube accelerated real-time quantitative reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assay is reported for rapid detection of the severe acute respiratory syndrome coronavirus (SARS-CoV) replicase gene. A total of 49 samples (15 throat washes, 13 throat swabs, and 21 combined throat and nasal swabs) collected from patients admitted to the Hanoi-French and Ninhbinh hospitals in Vietnam during the SARS epidemic were evaluated and compared to conventional RT-PCR. The RT-LAMP assay demonstrated 100-fold-greater sensitivity, with a detection limit of 0.01 PFU. The sensitivity and specificity of RT-LAMP assay for detecting viral RNA in clinical specimens with regard to RT-PCR were 100 and 87%, respectively. The specificity of the RT-LAMP assay was further validated by restriction analysis as well as nucleotide sequencing of the amplified product. The concentration of virus in most of the clinical samples was 0.1 PFU (0.1 to 10 2 PFU), as determined from the standard curve of SARS RT-LAMP and based on the time of positivity. The assay procedure is quite simple, wherein the amplification is carried out in a single tube under isothermal conditions at 63°C, and the result can be obtained in less than 1 h (as early as 11 min). Thus, the RT-LAMP assay reported here has the advantages of rapid amplification, simple operation, and easy detection and will be useful for rapid and reliable clinical diagnosis of SARS-CoV in developing countries.
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Citations
Cites
Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
Self-citationIdentification of a novel coronavirus in patients with severe acute respiratory syndrome
Drosten, C., Günther, S., Preiser, W., van der Werf, S., Brodt, H.-R., Becker, S., Rabenau, H., Panning, M., Kolesnikova, L., Fouchier, R. A. M., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejmoa030747 · Cited by 4,574
A Novel Coronavirus Associated with Severe Acute Respiratory Syndrome
Ksiazek, T. G., Erdman, D., Goldsmith, C. S., Zaki, S. R., Peret, T., Emery, S., Tong, S., Urbani, C., Comer, J. A., Lim, W., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejmoa030781 · Cited by 4,344
Coronavirus as a possible cause of severe acute respiratory syndrome
Peiris, J., Lai, S., Poon, L., Guan, Y., Yam, L., Lim, W., Nicholls, J., Yee, W., Yan, W., Cheung, M., et al. (2003) · The Lancet · doi:10.1016/s0140-6736(03)13077-2 · Cited by 2,915
A Major Outbreak of Severe Acute Respiratory Syndrome in Hong Kong
Lee, N., Hui, D., Wu, A., Chan, P., Cameron, P., Joynt, G. M., Ahuja, A., Yung, M. Y., Leung, C. B., To, K. F., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejmoa030685 · Cited by 2,628
Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome
Rota, P. A., Oberste, M. S., Monroe, S. S., Nix, W. A., Campagnoli, R., Icenogle, J. P., Peñaranda, S., Bankamp, B., Maher, K., Chen, M.-H., et al. (2003) · Science · doi:10.1126/science.1085952 · Cited by 2,474
Peiris, J., Chu, C., Cheng, V., Chan, K., Hung, I., Poon, L., Law, K., Tang, B., Hon, T., Chan, C., et al. (2003) · The Lancet · doi:10.1016/s0140-6736(03)13412-5 · Cited by 2,375
Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections
Lennette, E. H., Lennette, D. A., & Lennette, E. T. (1989) · Virtual Defense Library (Ministerio de Defensa) · Cited by 2,353
Accelerated reaction by loop-mediated isothermal amplification using loop primers
Nagamine, K., Hase, T., & Notomi, T. (2002) · Molecular and Cellular Probes · doi:10.1006/mcpr.2002.0415 · Cited by 2,045
Self-citationThe Genome Sequence of the SARS-Associated Coronavirus
Marra, M. A., Jones, S. J. M., Astell, C. R., Holt, R. A., Brooks-Wilson, A., Butterfield, Y. S. N., Khattra, J., Asano, J. K., Barber, S. A., Chan, S. Y., et al. (2003) · Science · doi:10.1126/science.1085953 · Cited by 2,026
Cited by
Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection
Cheng, V. C. C., Lau, S. K. P., Woo, P. C. Y., & Yuen, K. Y. (2007) · Clinical Microbiology Reviews · doi:10.1128/cmr.00023-07 · Cited by 1,192
Mori, Y., & Notomi, T. (2009) · Journal of Infection and Chemotherapy · doi:10.1007/s10156-009-0669-9 · Cited by 1,015
Self-citationAssay Techniques and Test Development for COVID-19 Diagnosis
Carter, L. J., Garner, L. V., Smoot, J. W., Li, Y., Zhou, Q., Saveson, C. J., Sasso, J. M., Gregg, A. C., Soares, D. J., Beskid, T. R., et al. (2020) · ACS Central Science · doi:10.1021/acscentsci.0c00501 · Cited by 985
Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances
Kaneko, H., Kawana, T., Fukushima, E., & Suzutani, T. (2007) · Journal of Biochemical and Biophysical Methods · doi:10.1016/j.jbbm.2006.08.008 · Cited by 707
Tracking virus outbreaks in the twenty-first century
Grubaugh, N. D., Ladner, J. T., Lemey, P., Pybus, O. G., Rambaut, A., Holmes, E. C., & Andersen, K. G. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0296-2 · Cited by 485
Metadata
- Authors
- Thai, H. T. C., Le, M. Q., Vuong, C. D., Parida, M., Minekawa, H., Notomi, T., Hasebe, F., & Morita, K.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-05
- Volume
- 42
- Issue
- 5
- Pages
- 1956-1961
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2101258647
- Cited by
- 422
- References
- 21
- FWCI
- 11.44
- Type
- journal article
- Language
- en
- Open access
- green
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- Thai, H. T. C., Le, M. Q., Vuong, C. D., Parida, M., Minekawa, H., Notomi, T., Hasebe, F., & Morita, K. (2004). Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.42.5.1956-1961.2004
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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