The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Detection of Pseudomonas aeruginosa Producing Metallo-β-Lactamases in a Large Centralized Laboratory
Open accessAbstract
Metallo-β-lactamases (MBLs) have been increasingly recognized from clinical isolates worldwide, but the laboratory detection of these strains is not well defined. We report a study that developed an EDTA disk screen test and a molecular diagnostic assay for the detection of MBL-producing Pseudomonas aeruginosa . Using NCCLS disk methodology, inhibition zone diameters were determined in tests with imipenem (IPM) and meropenem (MEM) disks alone and in combination with 930 μg of EDTA. This test was compared with the MBL Etest. The duplex PCR assay showed 100% sensitivity and specificity for detecting MBL-producing control strains. Of the 241 clinical strains of IPM-nonsusceptible P. aeruginosa from the Calgary Health Region isolated from 2002 to 2004, 110/241 (46%) were MBL positive using phenotypic methods while 107/241 (45%) were PCR positive for MBL genes: 103/241 (43%) for bla VIM and 4/241 (2%) for bla IMP . The EDTA disk screen test using MEM showed 100% sensitivity and 97% specificity for detecting MBLs in control and clinical strains. The EDTA disk screen test is simple to perform and to interpret and can easily be introduced into the workflow of a clinical laboratory. We recommend that all IPM-nonsusceptible P. aeruginosa isolates be routinely screened for MBL production using the EDTA disk screen test and that PCR confirmation be performed at a regional laboratory.
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Cites
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Cited by
A systematic review: the current status of carbapenem resistance in East Africa
Ssekatawa, K., Byarugaba, D. K., Wampande, E., & Ejobi, F. (2018) · BMC Research Notes · doi:10.1186/s13104-018-3738-2 · Cited by 93
Kateete, D. P., Nakanjako, R., Namugenyi, J., Erume, J., Joloba, M. L., & Najjuka, C. F. (2016) · SpringerPlus · doi:10.1186/s40064-016-2986-7 · Cited by 84
Metadata
- Authors
- Pitout, J. D. D., Gregson, D. B., Poirel, L., McClure, J.-A., Le, P., & Church, D. L.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-07
- Volume
- 43
- Issue
- 7
- Pages
- 3129-3135
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2131185071
- Cited by
- 331
- References
- 32
- FWCI
- 4.18
- Type
- journal article
- Language
- en
- Open access
- green
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- Pitout, J. D. D., Gregson, D. B., Poirel, L., McClure, J.-A., Le, P., & Church, D. L. (2005). Detection of Pseudomonas aeruginosa Producing Metallo-β-Lactamases in a Large Centralized Laboratory. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.43.7.3129-3135.2005
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
