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Cited by 229HIV Research and TreatmentT-cell and Retrovirus StudiesHIV/AIDS Research and Interventions

The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.

Simultaneous Cell-to-Cell Transmission of Human Immunodeficiency Virus to Multiple Targets through Polysynapses

Open access
Rudnicka, Dominika, Feldmann, Jérôme, Porrot, Françoise, Wietgrefe, Steve, Guadagnini, Stéphanie, Prévost, Marie-Christine, Estaquier, Jérôme, Haase, Ashley T., Sol-Foulon, Nathalie, Schwartz, Olivier·2009·Journal of Virology
DOI 10.1128/jvi.00282-09PMC PMC2687379
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Abstract

Human immunodeficiency virus type 1 (HIV-1) efficiently propagates through cell-to-cell contacts, which include virological synapses (VS), filopodia, and nanotubes. Here, we quantified and characterized further these diverse modes of contact in lymphocytes. We report that viral transmission mainly occurs across VS and through “polysynapses,” a rosette-like structure formed between one infected cell and multiple adjacent recipients. Polysynapses are characterized by simultaneous HIV clustering and transfer at multiple membrane regions. HIV Gag proteins often adopt a ring-like supramolecular organization at sites of intercellular contacts and colocalize with CD63 tetraspanin and raft components GM1, Thy-1, and CD59. In donor cells engaged in polysynapses, there is no preferential accumulation of Gag proteins at contact sites facing the microtubule organizing center. The LFA-1 adhesion molecule, known to facilitate viral replication, enhances formation of polysynapses. Altogether, our results reveal an underestimated mode of viral transfer through polysynapses. In HIV-infected individuals, these structures, by promoting concomitant infection of multiple targets in the vicinity of infected cells, may facilitate exponential viral growth and escape from immune responses.

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Crossref · 10.1128/jvi.00282-09

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Metadata

Authors
Rudnicka, D., Feldmann, J., Porrot, F., Wietgrefe, S., Guadagnini, S., Prévost, M.-C., Estaquier, J., Haase, A. T., Sol-Foulon, N., & Schwartz, O.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Virus and Immunity Unit, Department of Virology, Institut Pasteur, URA CNRS 3015, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France
  • University of Minnesota (US)
  • Electron Microscopy Core Facility, Institut Pasteur, Paris, France
  • INSERM U841, Faculté de Médecine Henri Mondor, 8 rue du Général Sarrail, 94010 Créteil, France
Corresponding author
Schwartz, Olivier
ORCID
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
2009-06-15
Volume
83
Issue
12
Pages
6234-6246
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.00282-09
PMC
PMC2687379
OpenAlex
W2065794926
Cited by
229
References
54
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 13 · 2021: 10 · 2020: 11 · 2019: 8 · 2018: 10 · 2017: 7 · 2016: 14 · 2015: 10 · 2014: 26 · 2013: 26 · 2012: 22
FWCI
10.04
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Virology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
  • Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
Keywords
HIV-1, cell-to-cell transmission, virological synapse, filopodia, nanotubes, CD63, lipid rafts, LFA-1, lymphocytes
MeSH terms
Animals, Female, Humans, Macaca, Microscopy, Electron, Scanning, Pseudopodia, Virus Replication, CD4-Positive T-Lymphocytes, HIV-1, HIV Infections, Lymphocyte Function-Associated Antigen-1, Jurkat Cells, Microscopy, Electron, Transmission, env Gene Products, Human Immunodeficiency Virus, gag Gene Products, Human Immunodeficiency Virus

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