The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Simultaneous Cell-to-Cell Transmission of Human Immunodeficiency Virus to Multiple Targets through Polysynapses
Open accessAbstract
Human immunodeficiency virus type 1 (HIV-1) efficiently propagates through cell-to-cell contacts, which include virological synapses (VS), filopodia, and nanotubes. Here, we quantified and characterized further these diverse modes of contact in lymphocytes. We report that viral transmission mainly occurs across VS and through “polysynapses,” a rosette-like structure formed between one infected cell and multiple adjacent recipients. Polysynapses are characterized by simultaneous HIV clustering and transfer at multiple membrane regions. HIV Gag proteins often adopt a ring-like supramolecular organization at sites of intercellular contacts and colocalize with CD63 tetraspanin and raft components GM1, Thy-1, and CD59. In donor cells engaged in polysynapses, there is no preferential accumulation of Gag proteins at contact sites facing the microtubule organizing center. The LFA-1 adhesion molecule, known to facilitate viral replication, enhances formation of polysynapses. Altogether, our results reveal an underestimated mode of viral transfer through polysynapses. In HIV-infected individuals, these structures, by promoting concomitant infection of multiple targets in the vicinity of infected cells, may facilitate exponential viral growth and escape from immune responses.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“It has also been reported that HIV-1-infected T cells can form multiple synapses [45]. In this case, the Gag protein accumulated in ring-shaped structures at sites of intercellular contact without MTOC polarization.”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [45]
Citations
Cites
Vaccinia Virus Uses Macropinocytosis and Apoptotic Mimicry to Enter Host Cells
Mercer, J., & Helenius, A. (2008) · Science · doi:10.1126/science.1155164 · Cited by 788
Nguyen, D., & Hildreth, J. E. K. (2000) · Journal of Virology · doi:10.1128/jvi.74.7.3264-3272.2000 · Cited by 788
Spread of HTLV-I Between Lymphocytes by Virus-Induced Polarization of the Cytoskeleton
Igakura, T., Stinchcombe, J. C., Goon, P. K. C., Taylor, G. P., Weber, J. N., Griffiths, G. M., Tanaka, Y., Osame, M., & Bangham, C. R. M. (2003) · Science · doi:10.1126/science.1080115 · Cited by 755
Sowinski, S., Jolly, C., Berninghausen, O., Purbhoo, M. A., Chauveau, A., Köhler, K., Oddos, S., Eissmann, P., Brodsky, F. M., Hopkins, C., et al. (2008) · Nature Cell Biology · doi:10.1038/ncb1682 · Cited by 754
Recruitment of HIV and Its Receptors to Dendritic Cell-T Cell Junctions
McDonald, D., Wu, L., Bohks, S. M., KewalRamani, V. N., Unutmaz, D., & Hope, T. J. (2003) · Science · doi:10.1126/science.1084238 · Cited by 707
Plasma membrane rafts play a critical role in HIV-1 assembly and release
Ono, A., & Freed, E. O. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.241320298 · Cited by 700
T-cell-antigen recognition and the immunological synapse
Huppa, J. B., & Davis, M. M. (2003) · Nature reviews. Immunology · doi:10.1038/nri1245 · Cited by 683
HIV-1 Cell to Cell Transfer across an Env-induced, Actin-dependent Synapse
Jolly, C., Kashefi, K., Hollinshead, M., & Sattentau, Q. J. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20030648 · Cited by 623
Avoiding the void: Cell-to-cell spread of human viruses
Sattentau, Q. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1972 · Cited by 614
Setting the stage: host invasion by HIV
Hladik, F., & McElrath, M. J. (2008) · Nature reviews. Immunology · doi:10.1038/nri2302 · Cited by 611
Cited by
Sorted by citations
Targeting early infection to prevent HIV-1 mucosal transmission
Haase, A. T. (2010) · Nature · doi:10.1038/nature08757 · Cited by 591
Self-citationLou, E., Fujisawa, S., Morozov, A., Barlas, A., Romin, Y., Dogan, Y., Gholami, S., Moreira, A. L., Manova-Todorova, K., & Moore, M. A. S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033093 · Cited by 420
Virus cell-to-cell transmission
Mothes, W., Sherer, N. M., Jin, J., & Zhong, P. (2010) · Journal of Virology · doi:10.1128/jvi.00443-10 · Cited by 353
Pais-Correia, A.-M., Sachse, M., Guadagnini, S., Robbiati, V., Lasserre, R., Gessain, A., Gout, O., Alcover, A., & Thoulouze, M.-I. (2010) · Nature Medicine · doi:10.1038/nm.2065 · Cited by 344
Self-citationHIV-infected T cells are migratory vehicles for viral dissemination
Murooka, T. T., Deruaz, M., Marangoni, F., Vrbanac, V. D., Seung, E., von Andrian, U. H., Tager, A. M., Luster, A. D., & Mempel, T. R. (2012) · Nature · doi:10.1038/nature11398 · Cited by 322
Metadata
- Authors
- Rudnicka, D., Feldmann, J., Porrot, F., Wietgrefe, S., Guadagnini, S., Prévost, M.-C., Estaquier, J., Haase, A. T., Sol-Foulon, N., & Schwartz, O.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Virus and Immunity Unit, Department of Virology, Institut Pasteur, URA CNRS 3015, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France
- University of Minnesota (US)
- Electron Microscopy Core Facility, Institut Pasteur, Paris, France
- INSERM U841, Faculté de Médecine Henri Mondor, 8 rue du Général Sarrail, 94010 Créteil, France
- Corresponding author
- Schwartz, Olivier
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2009-06-15
- Volume
- 83
- Issue
- 12
- Pages
- 6234-6246
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC2687379
- OpenAlex
- W2065794926
- Cited by
- 229
- References
- 54
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 13 · 2021: 10 · 2020: 11 · 2019: 8 · 2018: 10 · 2017: 7 · 2016: 14 · 2015: 10 · 2014: 26 · 2013: 26 · 2012: 22
- FWCI
- 10.04
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
- Keywords
- HIV-1, cell-to-cell transmission, virological synapse, filopodia, nanotubes, CD63, lipid rafts, LFA-1, lymphocytes
- MeSH terms
- Animals, Female, Humans, Macaca, Microscopy, Electron, Scanning, Pseudopodia, Virus Replication, CD4-Positive T-Lymphocytes, HIV-1, HIV Infections, Lymphocyte Function-Associated Antigen-1, Jurkat Cells, Microscopy, Electron, Transmission, env Gene Products, Human Immunodeficiency Virus, gag Gene Products, Human Immunodeficiency Virus
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
