Equus caballus Major Histocompatibility Complex Class I Is an Entry Receptor for Equine Herpesvirus Type 1
Open accessAbstract
In this study, Equus caballus major histocompatibility complex class I (MHC-I) was identified as a cellular entry receptor for the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This novel EHV-1 receptor was discovered using a cDNA library from equine macrophages. cDNAs from this EHV-1-susceptible cell type were inserted into EHV-1-resistant B78H1 murine melanoma cells, these cells were infected with an EHV-1 lacZ reporter virus, and cells that supported virus infection were identified by X-Gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) staining. Positive cells were subjected to several rounds of purification to obtain homogeneous cell populations that were shown to be uniformly infected with EHV-1. cDNAs from these cell populations were amplified by PCR and then sequenced. The sequence data revealed that the EHV-1-susceptible cells had acquired an E. caballus MHC-I cDNA. Cell surface expression of this receptor was verified by confocal immunofluorescence microscopy. The MHC-I cDNA was cloned into a mammalian expression vector, and stable B78H1 cell lines were generated that express this receptor. These cell lines were susceptible to EHV-1 infection while the parental B78H1 cells remained resistant to infection. In addition, EHV-1 infection of the B78H1 MHC-I-expressing cell lines was inhibited in a dose-dependent manner by an anti-MHC-I antibody.
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MHC I has been identified as a functional entry receptor for gD in equine dermal and brain microvascular endothelial cells.
“MHC I has been identified as a functional entry receptor for gD in equine dermal and brain microvascular EC (Kurtz et al., 2010; Sasaki et al., 2011).”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Kurtz et al., 2010
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- Authors
- Kurtz, B. M., Singletary, L. B., Kelly, S. D., & Frampton, A. R.
- Affiliations
- University of North Carolina Wilmington (US)
- Corresponding author
- Frampton, Arthur R.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2010-09-15
- Volume
- 84
- Issue
- 18
- Pages
- 9027-9034
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC2937649
- OpenAlex
- W2018324683
- Cited by
- 44
- References
- 49
- Citations by year
- 2024: 1 · 2023: 4 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 5 · 2013: 2 · 2012: 4
- FWCI
- 2.79
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Grayson-Jockey Club Research Foundation
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
- Keywords
- equine herpesvirus 1, horses, MHC class I, alphaherpesvirus, receptor identification, viral susceptibility
- MeSH terms
- Animals, beta-Galactosidase, Cell Line, Cell Membrane, Herpesvirus 1, Equid, Horses, Macrophages, Microscopy, Fluorescence, Receptors, Virus, Staining and Labeling, Transfection, Histocompatibility Antigens Class I, Gene Library, Gene Expression, Sequence Analysis, DNA, Genes, Reporter, Microscopy, Confocal, Mice, Virus Internalization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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