Cited by 44Herpesvirus Infections and TreatmentsAnimal Virus Infections StudiesViral-associated cancers and disorders

Equus caballus Major Histocompatibility Complex Class I Is an Entry Receptor for Equine Herpesvirus Type 1

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Kurtz, Brian M., Singletary, Lauren B., Kelly, Sean D., Frampton, Arthur R.·2010·Journal of Virology

Abstract

In this study, Equus caballus major histocompatibility complex class I (MHC-I) was identified as a cellular entry receptor for the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This novel EHV-1 receptor was discovered using a cDNA library from equine macrophages. cDNAs from this EHV-1-susceptible cell type were inserted into EHV-1-resistant B78H1 murine melanoma cells, these cells were infected with an EHV-1 lacZ reporter virus, and cells that supported virus infection were identified by X-Gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) staining. Positive cells were subjected to several rounds of purification to obtain homogeneous cell populations that were shown to be uniformly infected with EHV-1. cDNAs from these cell populations were amplified by PCR and then sequenced. The sequence data revealed that the EHV-1-susceptible cells had acquired an E. caballus MHC-I cDNA. Cell surface expression of this receptor was verified by confocal immunofluorescence microscopy. The MHC-I cDNA was cloned into a mammalian expression vector, and stable B78H1 cell lines were generated that express this receptor. These cell lines were susceptible to EHV-1 infection while the parental B78H1 cells remained resistant to infection. In addition, EHV-1 infection of the B78H1 MHC-I-expressing cell lines was inhibited in a dose-dependent manner by an anti-MHC-I antibody.

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Crossref · 10.1128/jvi.00287-10

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  • MHC I has been identified as a functional entry receptor for gD in equine dermal and brain microvascular endothelial cells.

    “MHC I has been identified as a functional entry receptor for gD in equine dermal and brain microvascular EC (Kurtz et al., 2010; Sasaki et al., 2011).”

    From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Kurtz et al., 2010

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Authors
Kurtz, B. M., Singletary, L. B., Kelly, S. D., & Frampton, A. R.
Affiliations
University of North Carolina Wilmington (US)
Corresponding author
Frampton, Arthur R.
Venue
Journal of Virology
Published
2010-09-15
Volume
84
Issue
18
Pages
9027-9034
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
OpenAlex
W2018324683
Cited by
44
References
49
Citations by year
2024: 1 · 2023: 4 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 5 · 2013: 2 · 2012: 4
FWCI
2.79
Type
journal article
Language
en
Open access
Locations
  • Journal of Virology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Grayson-Jockey Club Research Foundation
Topics
  • Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
Keywords
equine herpesvirus 1, horses, MHC class I, alphaherpesvirus, receptor identification, viral susceptibility
MeSH terms
Animals, beta-Galactosidase, Cell Line, Cell Membrane, Herpesvirus 1, Equid, Horses, Macrophages, Microscopy, Fluorescence, Receptors, Virus, Staining and Labeling, Transfection, Histocompatibility Antigens Class I, Gene Library, Gene Expression, Sequence Analysis, DNA, Genes, Reporter, Microscopy, Confocal, Mice, Virus Internalization

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