Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D: MHC class I acts as a EHV-1gD receptor
Open accessAbstract
The endotheliotropism of equine herpesvirus-1 (EHV-1) leads to encephalomyelitis secondary to vasculitis and thrombosis in the infected horse central nervous system (CNS). To identify the host factors involved in EHV-1 infection of CNS endothelial cells, we performed functional cloning using an equine brain microvascular endothelial cell cDNA library. Exogenous expression of equine major histocompatibility complex (MHC) class I heavy chain genes conferred susceptibility to EHV-1 infection in mouse NIH3T3 cells, which are not naturally susceptible to EHV-1 infection. Equine MHC class I molecules bound to EHV-1 glycoprotein D (gD), and both anti-gD antibodies and a soluble form of gD blocked viral entry into NIH3T3 cells stably expressing the equine MHC class I heavy chain gene (3T3-A68 cells). Treatment with an anti-equine MHC class I monoclonal antibody blocked EHV-1 entry into 3T3-A68 cells, equine dermis (E. Derm) cells and equine brain microvascular endothelial cells. In addition, inhibition of cell surface expression of MHC class I molecules in E. Derm cells drastically reduced their susceptibility to EHV-1 infection. These results suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells.
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MHC I has been identified as a functional entry receptor for gD in equine dermal and brain microvascular endothelial cells.
“MHC I has been identified as a functional entry receptor for gD in equine dermal and brain microvascular EC (Kurtz et al., 2010; Sasaki et al., 2011).”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Sasaki et al., 2011
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The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1
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- Authors
- Sasaki, M., Hasebe, R., Makino, Y., Suzuki, T., Fukushi, H., Okamoto, M., Matsuda, K., Taniyama, H., Sawa, H., & Kimura, T.
- Affiliations
- Hokkaido University (JP)
- Gifu University (JP)
- Rakuno Gakuen University (JP)
- Corresponding author
- Kimura, Takashi
- ORCID
- Venue
- Genes to Cells
- Publisher
- Wiley
- Published
- 2011-02-10
- Volume
- 16
- Issue
- 4
- Pages
- 343-357
- ISSN
- 1356-9597 (ISSN-L)
- PMC
- PMC3118799
- OpenAlex
- W2121067996
- Cited by
- 46
- References
- 46
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 1 · 2019: 7 · 2018: 2 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 4 · 2012: 4
- FWCI
- 3.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes to Cells (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Hokkaido University Collection of Scholarly and Academic Papers (Hokkaido University) (submittedVersion, cc-by-nc-sa)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Funders
- University of California, San Diego
- Japan Society for the Promotion of Science (23790454)
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Keywords
- equine herpesvirus 1, MHC class I, glycoprotein D, viral entry receptor, endothelial cells, encephalomyelitis, central nervous system infection, host factors, MHC class I heavy chain, viral entry inhibition
- MeSH terms
- Animals, Herpesvirus 1, Equid, Genes, MHC Class I, Genetic Testing, Herpesviridae Infections, Horse Diseases, Horses, Molecular Sequence Data, Viral Envelope Proteins, NIH 3T3 Cells, Endothelial Cells, Mice
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