Innate immune responses of airway epithelial cells to infection with Equine herpesvirus-1

Soboll Hussey, Gisela; Ashton, Laura V.; Quintana, Ayshea M.; Lunn, David P.; Goehring, Lutz S.; Annis, Kristina; Landolt, Gabriele

doi:10.1016/j.vetmic.2014.01.018

Cited by 37Herpesvirus Infections and TreatmentsImmune Response and Inflammationinterferon and immune responses

Innate immune responses of airway epithelial cells to infection with Equine herpesvirus-1

Soboll Hussey, Gisela, Ashton, Laura V., Quintana, Ayshea M., Lunn, David P., Goehring, Lutz S., Annis, Kristina, Landolt, Gabriele·2014·Veterinary Microbiology

DOI 10.1016/j.vetmic.2014.01.018

Open this paper in CoChat — free account

Abstract

Equine herpesvirus-1 (EHV-1) is the cause of respiratory disease, abortion and myelitis in horses worldwide. Protection following infection or vaccination is typically incomplete and this lack of protective immunity is thought to be due to the immunomodulatory properties of EHV-1. EHV-1 immune modulation is likely initiated early in the infection cycle at the respiratory epithelium, but to date, immunity to EHV-1 at the epithelial cell barrier remains poorly characterized. Thus, the purpose of this study was to use a recently established primary equine respiratory epithelial cell culture (EREC) system to characterize innate immunity to EHV-1. Differentiated ERECs were inoculated with a neuropathogenic strain of EHV-1 and cytokine responses were determined using quantitative real-time polymerase chain reaction and ELISA. Major histocompatibility complex (MHC)-I and MHC-II as well as toll-like receptor (TLR)3 and TLR9 protein expression were examined using fluorescence activated cell-sorting analysis and chemotaxis of neutrophils and monocytes were evaluated using chemotaxis assays. Infection with EHV-1 resulted in increased expression of TLR3 and 9 as well as inflammatory cytokines (IL-1, TNF-alpha, IFN-alpha, and IL-6) and chemokines (IL-8, MCP-1). In contrast, EHV-1 infection caused marked decreases of MHC-I and MHC-II expression as well as a reduction in IFN-gamma production. In summary, these results provide an initial characterization of the early immune response to EHV-1…

Integrity signals

Registry

Crossref · 10.1016/j.vetmic.2014.01.018

Cites

44 of 44 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

EHV-1 activated the interferon response in the upper respiratory tract by upregulating the expression of IFN-α mRNA and protein levels in equine respiratory epithelial cells and respiratory mucosal explants.
“EHV-1 activated the IFN response in the URT by upregulating the expression of IFN-α mRNA and protein levels in EREC and respiratory mucosal explants (Hussey et al., 2014; Poelaert et al., 2018).”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Hussey et al., 2014
EHV-1 early protein pUL56 modulates cytokine responses both in vivo and in vitro.
“Besides, EHV-1 early protein pUL56 (encoded by EHV-1 ORF1) has also been shown to modulate the cytokine responses both in vivo and in vitro (Soboll Hussey et al., 2011; Hussey et al., 2014).”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Hussey et al., 2014

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · see citing works
Regulation of Adaptive Immunity by the Innate Immune System
Iwasaki, A., & Medzhitov, R. M. (2010) · Science · doi:10.1126/science.1183021 · Cited by 2,139 · see citing works
Infectious diseases of livestock
Coetzer, J. A. W., & Tustin, R. C. (2004) · Oxford University Press eBooks · Cited by 1,226 · see citing works
Pattern Recognition Receptors and the Innate Immune Response to Viral Infection
Thompson, M., Kaminski, J. J., Kurt‐Jones, E. A., & Fitzgerald, K. A. (2011) · Viruses · doi:10.3390/v3060920 · Cited by 845 · see citing works
Two-Step Red-Mediated Recombination for versatile High-Efficiency Markerless DNA Manipulation in Escherichia Coli
Karstentischer, B., von Einem, J., Kaufer, B. B., & Osterrieder, N. (2006) · BioTechniques · doi:10.2144/000112096 · Cited by 790 · see citing works
Welcome to the neighborhood: epithelial cell‐derived cytokines license innate and adaptive immune responses at mucosal sites
Saenz, S. A., Taylor, B. C., & Artis, D. (2008) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00713.x · Cited by 484 · see citing works
Beyond inflammation: airway epithelial cells are at the interface of innate and adaptive immunity
Kato, A., & Schleimer, R. P. (2007) · Current Opinion in Immunology · doi:10.1016/j.coi.2007.08.004 · Cited by 337 · see citing works
A point mutation in a herpesvirus polymerase determines neuropathogenicity.
Goodman, L. B., Loregian, A., Perkins, G. A., Nugent, J., Buckles, E. L., Mercorelli, B., Kydd, J. H., Palù, G., Smith, K. C., Osterrieder, N., et al. (2007) · PLoS Pathogens · doi:10.1371/journal.ppat.0030160 · Cited by 223 · see citing works
Cytokines in innate host defense in the lung
Strieter, R. M., Belperio, J. A., & Keane, M. P. (2002) · Journal of Clinical Investigation · doi:10.1172/jci15277 · Cited by 198 · see citing works
Varicelloviruses avoid T cell recognition by UL49.5-mediated inactivation of the transporter associated with antigen processing
Koppers‐Lalic, D., Reits, E. A., Ressing, M. E., Lipińska, A. D., Abele, R., Koch, J., Rezende, M. M., Admiraal, P. C., van Leeuwen, D., Bieńkowska-Szewczyk, K., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501463102 · Cited by 187 · see citing works
Development of a bead-based multiplex assay for simultaneous quantification of cytokines in horses
Wagner, B., & Freer, H. (2008) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2008.10.313 · Cited by 159 · see citing works
Cytokines in innate host defense in the lung
Strieter, R. M., Belperio, J. A., & Keane, M. P. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215277 · Cited by 158 · see citing works
Report of the Second Equine Leucocyte Antigen Workshop, Squaw Valley, California, July 1995
Lunn, D. P., Holmes, M. A., Antczak, D. F., Agerwal, N., Baker, J., Bendali-Ahcéne, S., Blanchard-Channell, M., Byrne, K. M., Cannizzo, K., Davis, W. C., et al. (1998) · Veterinary Immunology and Immunopathology · doi:10.1016/s0165-2427(97)00160-8 · Cited by 141 · see citing works
Self-citation
Correlations between Lung Proinflammatory Cytokine Levels, Virus Replication, and Disease after Swine Influenza Virus Challenge of Vaccination-Immune Pigs
Reeth, K. V., Gucht, S. V., & Pensaert, M. B. (2002) · Viral Immunology · doi:10.1089/088282402320914520 · Cited by 136 · see citing works
Risk factors for development of neurologic disease after experimental exposure to equine herpesvirus-1 in horses.
Allen, G. P. (2008) · American Journal of Veterinary Research · doi:10.2460/ajvr.69.12.1595 · Cited by 135 · see citing works
Chapter 1 TSLP in Epithelial Cell and Dendritic Cell Cross Talk
Liu, Y. (2009) · Advances in immunology · doi:10.1016/s0065-2776(08)01001-8 · Cited by 109 · see citing works
Detection and Quantification of Equine Herpesvirus-1 Viremia and Nasal Shedding by Real-Time Polymerase Chain Reaction
Hussey, S. B., Clark, R., Lunn, K. F., Breathnach, C. C., Hussey, G. S., Whalley, J. M., & Lunn, D. P. (2006) · Journal of Veterinary Diagnostic Investigation · doi:10.1177/104063870601800403 · Cited by 100 · see citing works
Self-citation
THE ROLE OF EPITHELIAL β -DEFENSINS AND CATHELICIDINS IN HOST DEFENSE OF THE LUNG
Hiemstra, P. S. (2007) · Experimental Lung Research · doi:10.1080/01902140701756687 · Cited by 93 · see citing works
Pre-infection frequencies of equine herpesvirus-1 specific, cytotoxic T lymphocytes correlate with protection against abortion following experimental infection of pregnant mares
Kydd, J. H., Wattrang, E., & Hannant, D. (2003) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2003.08.004 · Cited by 91 · see citing works
Airway Epithelial Cells Regulate the Functional Phenotype of Locally Differentiating Dendritic Cells: Implications for the Pathogenesis of Infectious and Allergic Airway Disease
Rate, A., Upham, J. W., Bosco, A., McKenna, K. L., & Holt, P. G. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.182.1.72 · Cited by 88 · see citing works
A Single‐Nucleotide Polymorphism in a Herpesvirus DNA Polymerase Is Sufficient to Cause Lethal Neurological Disease
de Walle, G. R. V., Goupil, R., Wishon, C., DAMIANI, A. M., Perkins, G. A., & Osterrieder, N. (2009) · The Journal of Infectious Diseases · doi:10.1086/599316 · Cited by 83 · see citing works
Evaluation of immune responses following infection of ponies with an EHV-1 ORF1/2 deletion mutant.
Hussey, G. S., Hussey, S. B., Wagner, B., Horohov, D. W., Van de Walle, G. R., Osterrieder, N., Goehring, L. S., Rao, S., & Lunn, D. P. (2011) · Veterinary Research · doi:10.1186/1297-9716-42-23 · Cited by 73 · see citing works
Self-citation
Rhinovirus Infection Induces Major Histocompatibility Complex Class I and Costimulatory Molecule Upregulation on Respiratory Epithelial Cells
Papi, A. A., Stanciu, L. A., Papadopoulos, N. G., Teran, L. M., Holgate, S. T., & Johnston, S. L. (2000) · The Journal of Infectious Diseases · doi:10.1086/315463 · Cited by 73 · see citing works
Equine herpesvirus-1 infection induces IFN-γ production by equine T lymphocyte subsets
Breathnach, C. C., Hussey, G. S., Suresh, M., & Lunn, D. P. (2004) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2004.09.024 · Cited by 63 · see citing works
Self-citation
Herpesvirus chemokine-binding glycoprotein G (gG) efficiently inhibits neutrophil chemotaxis in vitro and in vivo.
Van de Walle, G. R., May, M. L., Sukhumavasi, W., von Einem, J., & Osterrieder, N. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.6.4161 · Cited by 55 · see citing works
Summary Report of the Second Equine Leucocyte Antigen Workshop
Lunn, D. P., Holmes, M. A., & Antczak, D. F. (1996) · Veterinary Immunology and Immunopathology · doi:10.1016/s0165-2427(96)05674-7 · Cited by 55 · see citing works
Self-citation
Immunological characterization of the equine airway epithelium and of a primary equine airway epithelial cell culture model
Quintana, A. M., Landolt, G. A., Annis, K. M., & Hussey, G. S. (2011) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2010.12.008 · Cited by 55 · see citing works
Self-citation
Peptide transport activity of the transporter associated with antigen processing (TAP) is inhibited by an early protein of equine herpesvirus-1
Ambagala, A. P. N., Gopinath, R. S., & Srikumaran, S. (2004) · Journal of General Virology · doi:10.1099/vir.0.19563-0 · Cited by 54 · see citing works
Horse cytokine/IgG fusion proteins – mammalian expression of biologically active cytokines and a system to verify antibody specificity to equine cytokines
Wagner, B., Robeson, J., McCracken, M., Wattrang, E., & Antczak, D. F. (2005) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2004.11.010 · Cited by 54 · see citing works
Innate immune response and viral interference strategies developed by Human Herpesviruses
Vandevenne, P., Sadzot‐Delvaux, C., & Piette, J. G. (2010) · Biochemical Pharmacology · doi:10.1016/j.bcp.2010.07.001 · Cited by 52 · see citing works
Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
Hasebe, R., Sasaki, M., Sawa, H., Wada, R., Umemura, T., & Kimura, T. (2009) · Virology · doi:10.1016/j.virol.2009.07.032 · Cited by 51 · see citing works
Enhancement of Chemokine Function as an Immunomodulatory Strategy Employed by Human Herpesviruses
Viejo‐Borbolla, A., Martinez-Martín, N., Nel, H. J., Rueda, P., Martín, R., Blanco, S., Arenzana‐Seisdedos, F., Thelen, M., Fallon, P. G., & Alcamı́, A. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002497 · Cited by 51 · see citing works
Identification and Characterization of Equine Herpesvirus Type 1 pUL56 and Its Role in Virus-Induced Downregulation of Major Histocompatibility Complex Class I
Ma, G., Feineis, S., Osterrieder, N., & de Walle, G. R. V. (2012) · Journal of Virology · doi:10.1128/jvi.06994-11 · Cited by 49 · see citing works
Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D: MHC class I acts as a EHV-1gD receptor
Sasaki, M., Hasebe, R., Makino, Y., Suzuki, T., Fukushi, H., Okamoto, M., Matsuda, K., Taniyama, H., Sawa, H., & Kimura, T. (2011) · Genes to Cells · doi:10.1111/j.1365-2443.2011.01491.x · Cited by 46 · see citing works
<i>Equus caballus</i> Major Histocompatibility Complex Class I Is an Entry Receptor for Equine Herpesvirus Type 1
Kurtz, B. M., Singletary, L. B., Kelly, S. D., & Frampton, A. R. (2010) · Journal of Virology · doi:10.1128/jvi.00287-10 · Cited by 44 · see citing works
Severe Murine Lung Immunopathology Elicited by the Pathogenic Equine Herpesvirus 1 Strain RacL11 Correlates with Early Production of Macrophage Inflammatory Proteins 1α, 1β, and 2 and Tumor Necrosis Factor Alpha
Smith, P. M., Zhang, Y.-F., Grafton, W. D., Jennings, S., & O'Callaghan, D. (2000) · Journal of Virology · doi:10.1128/jvi.74.21.10034-10040.2000 · Cited by 34 · see citing works
CCL3 and Viral Chemokine-Binding Protein gG Modulate Pulmonary Inflammation and Virus Replication during Equine Herpesvirus 1 Infection
de Walle, G. R. V., Sakamoto, K., & Osterrieder, N. (2007) · Journal of Virology · doi:10.1128/jvi.02137-07 · Cited by 33 · see citing works
Alphaherpesviruses and Chemokines: Pas de Deux Not Yet Brought to Perfection
de Walle, G. R. V., Jarosinski, K. W., & Osterrieder, N. (2008) · Journal of Virology · doi:10.1128/jvi.00098-08 · Cited by 28 · see citing works
Equine Herpesvirus Type 4 UL56 and UL49.5 Proteins Downregulate Cell Surface Major Histocompatibility Complex Class I Expression Independently of Each Other
Said, A., Azab, W., DAMIANI, A. M., & Osterrieder, N. (2012) · Journal of Virology · doi:10.1128/jvi.00891-12 · Cited by 27 · see citing works
Expression of late viral proteins is restricted in nasal mucosal leucocytes but not in epithelial cells during early-stage equine herpes virus-1 infection.
Gryspeerdt, A. C., Vandekerckhove, A. P., Baghi, H. B., Van de Walle, G. R., & Nauwynck, H. J. (2012) · The Veterinary Journal · doi:10.1016/j.tvjl.2012.01.022 · Cited by 18 · see citing works
Analysis of the Herpesvirus Chemokine-binding Glycoprotein G Residues Essential for Chemokine Binding and Biological Activity
de Walle, G. R. V., Kaufer, B. B., Chbab, N., & Osterrieder, N. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m808127200 · Cited by 17 · see citing works
Human epithelial cells stimulated with Vibrio cholerae produce thymic stromal lymphopoietin and promote dendritic cell-mediated inflammatory Th2 response
Bhowmick, S., Chatterjee, D., & Chaudhuri, K. (2012) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2012.06.030 · Cited by 14 · see citing works
Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry
Platt, R., Sponseller, B. A., Chiang, Y.-W., & Roth, J. A. (2010) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2009.12.010 · Cited by 9 · see citing works
Intercellular Adhesion Molecule-1 (ICAM-1) and Lymphocyte Function-associated Antigen-1 (LFA-1) Contribute to the Elimination of Equine Herpesvirus Type 1 (EHV-1) from the Lungs of Intranasally Infected BALB/c Mice
Maeda, K., Kazushige, K. A. I., Hayashi, T., Hasegawa, K., & Matsumura, T. (2004) · Journal of Comparative Pathology · doi:10.1016/j.jcpa.2003.10.005 · Cited by 2 · see citing works

Flagged first, then by citations

Sorted by citations

The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1
Laval, K., Poelaert, K. C. K., Van Cleemput, J., Zhao, J., Vandekerckhove, A. P., Gryspeerdt, A. C., Garré, B., van der Meulen, K., Baghi, H. B., Dubale, H. N., et al. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.662686 · Cited by 54 · see citing works
Contentious host–microbiota relationship in inflammatory bowel disease – can foes become friends again?
Satokari, R. M. (2014) · Scandinavian Journal of Gastroenterology · doi:10.3109/00365521.2014.966320 · Cited by 39 · see citing works
Equine Herpesvirus-1 Myeloencephalopathy
Pusterla, N., Hussey, G. S., & Goehring, L. S. (2022) · Veterinary Clinics of North America Equine Practice · doi:10.1016/j.cveq.2022.05.006 · Cited by 30 · see citing works
Self-citation
Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells.
Soboll Hussey, G., Ashton, L. V., Quintana, A. M., Van de Walle, G. R., Osterrieder, N., & Lunn, D. P. (2014) · Virology · doi:10.1016/j.virol.2014.05.023 · Cited by 28 · see citing works
Self-citation
Viral replication and innate immunity of feline herpesvirus-1 virulence-associated genes in feline respiratory epithelial cells
Lee, Y., Maes, R. K., Tai, S., & Hussey, G. S. (2019) · Virus Research · doi:10.1016/j.virusres.2019.02.013 · Cited by 28 · see citing works
Abortigenic but not neurotropic equine Herpes Virus 1 modulates the interferon antiviral defense.
Poelaert, K. C. K., Van Cleemput, J., Laval, K., Favoreel, H. W., Soboll Hussey, G., Maes, R. K., & Nauwynck, H. J. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00312 · Cited by 19 · see citing works
Use of a feline respiratory epithelial cell culture system grown at the air–liquid interface to characterize the innate immune response following feline herpesvirus 1 infection
Nelli, R. K., Maes, R. K., Kiupel, M., & Hussey, G. S. (2016) · Virus Research · doi:10.1016/j.virusres.2016.01.006 · Cited by 19 · see citing works
Equid Herpesvirus 1 targets the sensitization and induction steps to inhibit the type I interferon response in equine endothelial cells.
Oladunni, F. S., Sarkar, S., Reedy, S., Balasuriya, U. B. R., Horohov, D. W., & Chambers, T. M. (2019) · Journal of Virology · doi:10.1128/jvi.01342-19 · Cited by 18 · see citing works
Equine herpesvirus type 1 modulates inflammatory host immune response genes in equine endothelial cells
Johnstone, S., Barsova, J., Campos, I., & Frampton, A. R. (2016) · Veterinary Microbiology · doi:10.1016/j.vetmic.2016.06.012 · Cited by 18 · see citing works
Equine PBMC Cytokines Profile after In Vitro α- and γ-EHV Infection: Efficacy of a Parapoxvirus Ovis Based-Immunomodulator Treatment
Hue, E. S., Richard, E. A., Fortier, C. I., Fortier, G. D., Paillot, R., Raue, R., & Pronost, S. L. (2017) · Vaccines · doi:10.3390/vaccines5030028 · Cited by 17 · see citing works
Contribution of the immune response to the pathogenesis of equine herpesvirus-1 (EHV-1): Are there immune correlates that predict increased risk or protection from EHV-1 myeloencephalopathy?
Hussey, G. S., & Giessler, K. S. (2022) · The Veterinary Journal · doi:10.1016/j.tvjl.2022.105827 · Cited by 16 · see citing works
Impact of the host immune response on the development of equine herpesvirus myeloencephalopathy in horses
Giessler, K. S., Goehring, L. S., Jacob, S. I., Davis, A., Esser, M. M., Lee, Y., Zarski, L. M., Weber, P. S. D., & Hussey, G. S. (2024) · Journal of General Virology · doi:10.1099/jgv.0.001987 · Cited by 17 · see citing works
Self-citation
A Point Mutation in a Herpesvirus Co-Determines Neuropathogenicity and Viral Shedding
Franz, M., Goodman, L. B., de Walle, G. R. V., Osterrieder, N., & Greenwood, A. D. (2017) · Viruses · doi:10.3390/v9010006 · Cited by 15 · see citing works
Equine Herpesvirus type 1 modulates cytokine and chemokine profiles of mononuclear cells for efficient dissemination to target organs.
Pavulraj, S., Kamel, M., Stephanowitz, H., Liu, F., Plendl, J., Osterrieder, N., & Azab, W. (2020) · Viruses · doi:10.3390/v12090999 · Cited by 13 · see citing works
The innate immune response of equine bronchial epithelial cells is altered by training
Frellstedt, L., Gosset, P., Kervoaze, G., Hans, A., Desmet, C. J., Pirottin, D., Bureau, F., Lekeux, P. M., & Art, T. (2015) · Veterinary Research · doi:10.1186/s13567-014-0126-3 · Cited by 13 · see citing works
Transcriptomic Profiling of Equine and Viral Genes in Peripheral Blood Mononuclear Cells in Horses during Equine Herpesvirus 1 Infection
Zarski, L. M., Weber, P. S. D., Lee, Y., & Hussey, G. S. (2021) · Pathogens · doi:10.3390/pathogens10010043 · Cited by 12 · see citing works
Equine herpesvirus 1 elicits a strong pro-inflammatory response in the brain of mice
Mesquita, L. P., Costa, R. C., Zanatto, D. A., Bruhn, F. R. P., Mesquita, L. L. R., Lara, M. D. C. C. D. S. H., Villalobos, E. M. C., Massoco, C. D. O., Mori, C. M. C., Mori, Ê., et al. (2021) · Journal of General Virology · doi:10.1099/jgv.0.001556 · Cited by 12 · see citing works
Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge
Kim, S. K., Shakya, A. K., & O’Callaghan, D. J. (2016) · Journal of Virology · doi:10.1128/jvi.00986-16 · Cited by 9 · see citing works
Pathogenesis of Equid Alphaherpesvirus 1 Infection in the Central Nervous System of Mice
Mesquita, L. P., Costa, R. C., Mesquita, L. L. R., Lara, M. D. C. C. D. S. H., Villalobos, E. M. C., Mori, C. M. C., Mori, Ê., Howerth, E. W., & Maiorka, P. C. (2021) · Veterinary Pathology · doi:10.1177/03009858211020670 · Cited by 9 · see citing works
EHV-1 Pathogenesis: Current in vitro Models and Future Perspectives
Kamel, M. S., Pavulraj, S., Osterrieder, N., & Azab, W. (2019) · Frontiers in Veterinary Science · doi:10.3389/fvets.2019.00251 · Cited by 9 · see citing works
Development of Matrix-Embedded Bovine Tracheal Organoids to Study the Innate Immune Response against Bovine Respiratory Disease
Quah, P. S., Tran, B. M., Corbin, V., Chang, J. J. ‐., Wong, C. Y., Diaz-Méndez, A., Hartley, C. A., Zeng, W., Hanssen, E. G., Trifunovic, Z., et al. (2023) · Organoids · doi:10.3390/organoids2020007 · Cited by 8 · see citing works
A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1
Zarski, L. M., VAALA, W. E., Barnett, D. C., Bain, F. T., & Hussey, G. S. (2021) · Frontiers in Veterinary Science · doi:10.3389/fvets.2021.674850 · Cited by 7 · see citing works
Investigation of apoptosis in cultured cells infected with equine herpesvirus 1
Scrochi, M. R., Zanuzzi, C., Fuentealba, N. A., Nishida, F., Bravi, M. E., Pacheco, M. E., Sguazza, G. H., Gimeno, E., Portiansky, E. L., Muglia, C., et al. (2017) · Biotechnic & Histochemistry · doi:10.1080/10520295.2017.1359749 · Cited by 5 · see citing works
Investigation of the Systemic Antibody Response and Antigen Detection Following Intranasal Administration of Two Commercial Equine Herpesvirus-1 Vaccines to Adult Horses
Spann, K., Barnum, S. M., & Pusterla, N. (2023) · Journal of Equine Veterinary Science · doi:10.1016/j.jevs.2023.104229 · Cited by 5 · see citing works
Influence of Age and Immunostimulation on the Level of Toll-Like Receptor Gene (TLR3, 4, and 7) Expression in Foals
Migdał, A., Migdał, Ł., Oczkowicz, M., Okolski, A., & Chełmońska‐Soyta, A. (2020) · Animals · doi:10.3390/ani10111966 · Cited by 4 · see citing works
Equine Herpesvirus Infections: Treatment Progress and Challenges in Horses and Donkeys
Khan, M. Z., Ji, Y., Fan, X., Liu, Y., Liu, W., & Wang, C. (2025) · Veterinary Sciences · doi:10.3390/vetsci12111082 · Cited by 5 · see citing works
Attenuation of the neuropathogenic equine herpesvirus type 1 strain Ab4p in hamsters by a single amino acid mutation (D752N) in viral DNA polymerase ORF30
Nishimura, F., Fukushi, N., Sakai, H., & Fukushi, H. (2024) · Journal of Veterinary Medical Science · doi:10.1292/jvms.24-0338 · Cited by 2 · see citing works
Alfaherpesvírus equino: medidas de controle em casos de surtos da doença
MACHADO, L. J. F., Ferreira, L. C. D. S., Antonucci, A. M., Gerardi, B., Albernaz, R. M., & de Almeida, T. L. (2023) · Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP · doi:10.36440/recmvz.v21.38438 · Cited by 2 · see citing works
Clinical effect of three important cytokines on the pathogenesis of equine herpes virus 9 (EHV-9), equine herpes virus 1 (EHV-1) and Zebra borne EHV-1
Saleh, A. G. (2017) · IOSR Journal of Agriculture and Veterinary Science · doi:10.9790/2380-1003024857 · Cited by 2 · see citing works
Herpesvirus equino 1: evaluación de la apoptosis en cultivo de células heterólogas y homólogas, y en pulmón de ratones BALB/c, como mecanismo patogénico y de evasión de la respuesta inmune del hospedador
Scrochi, M. R. (2016) · doi:10.35537/10915/53833
Avaliação da resposta inflamatória no sistema nervoso central causada pelo herpesvírus equino tipo 1 utilizando um modelo murino de neuroinfecção
Tonietti, P. D. O. (2017) · doi:10.11606/t.10.2017.tde-21022017-151720
Development and Characterization of a Live-attenuated Vaccine to Combat Equine Herpesvirus Type-1 infections
Liu, S. (2015) · doi:10.31390/gradschool_dissertations.3816
Modulation of Inflammatory Cytokine, Chemokine, and Toll-Like Genes and Transcriptome Analysis of Equine Endothelial Cells Following Infection with Equid Herpesvirus-1, and Equine Arteritis Virus.
Dunuwille, S. W. (2019) · UKnowledge (University of Kentucky) · doi:10.13023/etd.2019.388
Characterizing and localizing EHV-1 chronic-persistent infection in the natural host
Samoilowa, S. (2019) · Elektronische Hochschulschriften der LMU München (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.24696
Amélioration des protocoles vaccinaux contre la grippe équine et la rhinopneumonie : apport de l’iPPVO en tant qu’adjuvant dans le modèle équin, nouvelle approche de la mesure des anticorps neutralisants
Carnet, F. (2023) · theses.fr (ABES) · doi:10.70675/e61bea7cz4d28z4efdza802z0e0e8314b0a4
IFNL3 reduces replication of CIRDC-associated viruses in canine airway epithelial cells
Sharma, S., Berríos-Vázquez, G., Baldwin, K., Maes, R., & Hussey, G. S. (2026) · Virus Research · doi:10.1016/j.virusres.2026.199742
Differential Serum Cytokine Profiles in Aborting and Subclinically Infected Pregnant Mares with Equine Herpesvirus Type 1
Deniz, Ö., Ekinci, G., Çitil, M., Onmaz, A. C., Derelli, F. M., Timur, M. C., Altınbay, K., Mete, A., Yeğen, E., van den HOVEN, R., et al. (2026) · Animals · doi:10.3390/ani16162455

Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells.
Soboll Hussey, G., Ashton, L. V., Quintana, A. M., Van de Walle, G. R., Osterrieder, N., & Lunn, D. P. (2014) · Virology · doi:10.1016/j.virol.2014.05.023 · Cited by 28 · see citing works
Self-citation
A Live-Attenuated Equine Influenza Vaccine Stimulates Innate Immunity in Equine Respiratory Epithelial Cell Cultures That Could Provide Protection From Equine Herpesvirus 1
Zarski, L. M., VAALA, W. E., Barnett, D. C., Bain, F. T., & Hussey, G. S. (2021) · Frontiers in Veterinary Science · doi:10.3389/fvets.2021.674850 · Cited by 7 · see citing works
Mucosal T cell activation pathways are upregulated by equine herpesvirus type 1 infection
Holmes, C. M., Babasyan, S., & Wagner, B. (2026) · Veterinary Research · doi:10.1186/s13567-026-01741-x
Immune horses rapidly increase antileukoproteinase and lack type I interferon secretion during mucosal innate immune responses against equine herpesvirus type 1
Holmes, C. M., Babasyan, S., Eady, N. A., Schnabel, C. L., & Wagner, B. (2024) · Microbiology Spectrum · doi:10.1128/spectrum.01092-24 · Cited by 7 · see citing works
Equine herpesvirus-1 suppresses type-I interferon induction in equine endothelial cells.
Sarkar, S., Balasuriya, U. B. R., Horohov, D. W., & Chambers, T. M. (2015) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2015.07.015 · Cited by 22 · see citing works

Authors: Soboll Hussey, G., Ashton, L. V., Quintana, A. M., Lunn, D. P., Goehring, L. S., Annis, K., & Landolt, G.
Affiliations: Colorado State University (US)
North Carolina State University (US)
Ludwig-Maximilians-Universität München (DE)
ORCID: Ashton, Laura V.
Lunn, David P.
Goehring, Lutz S.
Venue: Veterinary Microbiology
Publisher: Elsevier BV
Published: 2014-05
Volume: 170
Issue: 1-2
Pages: 28-38
ISSN: 0378-1135 (ISSN-L)
DOI: 10.1016/j.vetmic.2014.01.018
OpenAlex: W2073077058
Cited by: 37
References: 44
Citations by year: 2026: 2 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 2 · 2019: 5 · 2018: 1 · 2017: 5 · 2016: 4 · 2015: 2 · 2014: 2
FWCI: 3.28
Type: journal article
Language: en
Open access: closed
Locations: Veterinary Microbiology (publishedVersion)
PubMed (publishedVersion)
Funders: Morris Animal Foundation
Institut National de la Recherche Agronomique
National Institute of Food and Agriculture
Topics: Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
Keywords: equine herpesvirus 1, innate immunity, airway epithelial cells, TLR3, TLR9, MHC class I, MHC class II, interferon-gamma, inflammatory cytokines, chemokines, neutrophil chemotaxis, monocyte chemotaxis, immunomodulation, horses, respiratory diseases
MeSH terms: Animals, Cells, Cultured, Epithelial Cells, Herpesvirus 1, Equid, Gene Expression Regulation, Herpesviridae Infections, Horse Diseases, Horses, Immunity, Innate, Cytokines, Chemokines, Respiratory Mucosa, Virus Internalization, Host-Pathogen Interactions

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Innate immune responses of airway epithelial cells to infection with Equine herpesvirus-1

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks