Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia.
Open accessAbstract
Results from Southern hybridization and PCR amplification experiments using a randomly synthesized reverse transcription-PCR product showed that peripheral blood leukocytes from horses showing no clinical signs of disease expressed a putative latency-associated transcript antisense to and overlapping the 3' end of the equid herpesvirus 1 (EHV-1) immediate-early gene (gene 64). A PCR product derived from this transcript has > or =96% identity with the published EHV-1 sequence. In situ hybridization studies of equine bronchial lymph nodes corroborated these findings and are consistent with reactivation data (D. A. Smith, A. Hamblin, and N. Edington, unpublished data), indicating that EHV-1 latency is established predominantly in CD5+/CD8+ leukocytes.
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Circulating T lymphocytes have been defined as a predominant site of EHV-1 latency.
“Finally, circulating T lymphocytes have been defined as a predominant site of EHV-1 latency.”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Chesters et al., 1997
Estimates that 1/50,000 peripheral blood mononuclear cells are latently infected with EHV-1 must be interpreted with caution because the authors failed to demonstrate the presence of latency-associated transcripts in these cells and/or confirm the findings obtained by Chesters and colleagues.
“However, these results must be interpreted with caution as the authors also failed to demonstrate the presence of LAT in these cells and/or confirm the findings obtained by Chesters and colleagues.”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Chesters et al., 1997
Peripheral blood mononuclear cells express a putative latency-associated transcript antisense to and overlapping the 3′ end of the EHV-1 immediate-early gene ORF64.
“Using southern hybridization and PCR techniques, Chesters and colleagues first demonstrated that PBMC express a putative LAT antisense to and overlapping the 3′ end of the EHV-1 immediate-early gene (ORF64) (Chesters et al., 1997).”
From The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1 (2021) · cites this paper as Chesters et al., 1997
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The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1
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- Authors
- Chesters, P. M., Allsop, R., Purewal, A., & Edington, N.
- Affiliations
- Royal Veterinary College (GB)
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1997-05
- Volume
- 71
- Issue
- 5
- Pages
- 3437-3443
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC191489
- OpenAlex
- W2163448246
- Cited by
- 86
- References
- 34
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 9 · 2018: 5 · 2017: 1 · 2016: 2 · 2015: 6 · 2014: 4 · 2013: 6
- FWCI
- 4.04
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
- Keywords
- equine herpesvirus 1, latency-associated transcript, herpesvirus latency, horses, peripheral blood leukocytes, trigeminal ganglion, immediate early genes, lymph nodes, viral reactivation, herpesvirus gene expression
- MeSH terms
- Animals, Herpesvirus 1, Equid, Horses, Leukocytes, RNA, Messenger, Trigeminal Ganglion, Blotting, Southern, Polymerase Chain Reaction, In Situ Hybridization, Virus Latency
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