The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Increased Immune Response Elicited by DNA Vaccination with a Synthetic gp120 Sequence with Optimized Codon Usage
Open accessAbstract
DNA vaccination elicits humoral and cellular immune responses and has been shown to confer protection against several viral, bacterial, and parasitic pathogens. Here we report that optimized codon usage of an injected DNA sequence considerably increases both humoral and cellular immune responses. We recently generated a synthetic human immunodeficiency virus type 1 gp120 sequence in which most wild-type codons were replaced with codons from highly expressed human genes (syngp120). In vitro expression of syngp120 is considerably increased in comparison to that of the respective wild-type sequence. In BALB/c mice, DNA immunization with syngp120 resulted in significantly increased antibody titers and cytotoxic T-lymphocyte reactivity, suggesting a direct correlation between expression levels and the immune response. Moreover, syngp120 is characterized by rev-independent expression and a low risk of recombination with viral sequences. Thus, synthetic genes with optimized codon usage represent a novel strategy to increase the efficacy and safety of DNA vaccination.
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Cites
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Cited by
DNA Vaccines: Immunology, Application, and Optimization
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High Guanine and Cytosine Content Increases mRNA Levels in Mammalian Cells
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Successful Immunization with a Single Injection of Non-integrating Lentiviral Vector
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Metadata
- Authors
- André, S., Seed, B., Eberle, J., Schraut, W., Bültmann, A., & Haas, J.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1998-02
- Volume
- 72
- Issue
- 2
- Pages
- 1497-1503
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2109347706
- Cited by
- 305
- References
- 60
- FWCI
- 3.92
- Type
- journal article
- Language
- en
- Open access
- bronze
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- André, S., Seed, B., Eberle, J., Schraut, W., Bültmann, A., & Haas, J. (1998). Increased Immune Response Elicited by DNA Vaccination with a Synthetic gp120 Sequence with Optimized Codon Usage. Journal of Virology. https://doi.org/10.1128/jvi.72.2.1497-1503.1998
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