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Cited by 136Viral Infections and Immunology ResearchVirology and Viral DiseasesEvolution and Genetic Dynamics

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

Genomic Features of Intertypic Recombinant Sabin Poliovirus Strains Excreted by Primary Vaccinees

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Cuervo, Nancy Stella, Guillot, Sophie, Romanenkova, Natalia, Combiescu, Mariana, Aubert-Combiescu, André, Seghier, Mohamed, Caro, Valérie, Crainic, Radu, Delpeyroux, Francis·2001·Journal of Virology
DOI 10.1128/jvi.75.13.5740-5751.2001PMC PMC114290
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Abstract

The trivalent oral poliomyelitis vaccine (OPV) contains three different poliovirus serotypes. It use therefore creates particularly favorable conditions for mixed infection of gut cells, and indeed intertypic vaccine-derived recombinants (VdRec) have been frequently found in patients with vaccine-associated paralytic poliomyelitis. Nevertheless, there have not been extensive searches for VdRec in healthy vaccinees following immunization with OPV. To determine the incidence of VdRec and their excretion kinetics in primary vaccinees, and to establish the general genomic features of the corresponding recombinant genomes, we characterized poliovirus isolates excreted by vaccinees following primary immunization with OPV. Isolates were collected from 67 children 2 to 60 days following vaccination. Recombinant strains were identified by multiple restriction fragment length polymorphism assays. The localization of junction sites in recombinant genomes was also determined. VdRec excreted by vaccinees were first detected 2 to 4 days after vaccination. The highest rate of recombinants was on day 14. The frequency of VdRec depends strongly on the serotype of the analyzed isolates (2, 53, and 79% of recombinant strains in the last-excreted type 1, 2, and 3 isolates, respectively). Particular associations of genomic segments were preferred in the recombinant genomes, and recombination junctions were found in the genomic region encoding the nonstructural proteins. Recombination junctions generally clustered in particular subgenomic regions that were dependent on the serotype of the isolate and/or on the associations of genomic segments in recombinants. Thus, VdRec are frequently excreted by vaccinees, and the poliovirus replication machinery requirements or selection factors appear to act in vivo to shape the features of the recombinant genomes.

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  1. Kew O. M. Nottay B. K. Evolution of the oral poliovaccine strains in humans occur by both mutation and intramolecular recombination.Modern approaches to vaccines. Chanock R. Lerner R. 1984 357 367 Cold Spring Harbor Press N.Y

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Metadata

Authors
Cuervo, N. S., Guillot, S., Romanenkova, N., Combiescu, M., Aubert-Combiescu, A., Seghier, M., Caro, V., Crainic, R., & Delpeyroux, F.
Affiliations
  • Institut Pasteur (FR)
  • Saint Petersburg Pasteur Institute (RU)
  • Institutul Cantacuzino (RO)
  • Institut Pasteur d'Algérie (DZ)
  • <!--label omitted: 1-->Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, Paris, France1;
ORCID
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
2001-07
Volume
75
Issue
13
Pages
5740-5751
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.75.13.5740-5751.2001
PMC
PMC114290
OpenAlex
W1990942102
Cited by
136
References
52
Citations by year
2025: 4 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 5 · 2015: 4 · 2014: 7 · 2013: 7 · 2012: 4
FWCI
5.88
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Virology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • World Health Organization
  • European Commission
Topics
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
Keywords
trivalent oral polio vaccine, excretion kinetics, recombination junctions, non-structural proteins, poliovirus serotypes, co-infection, vaccine-associated paralytic poliomyelitis
MeSH terms
Animals, Chlorocebus aethiops, Child, Humans, Poliovirus Vaccine, Oral, Recombination, Genetic, Polymorphism, Restriction Fragment Length, RNA, Viral, Vero Cells, Genome, Viral, Poliovirus, Reverse Transcriptase Polymerase Chain Reaction

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