The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Lethality to Ferrets of H5N1 Influenza Viruses Isolated from Humans and Poultry in 2004
Open accessAbstract
The 2004 outbreaks of H5N1 influenza viruses in Vietnam and Thailand were highly lethal to humans and to poultry; therefore, newly emerging avian influenza A viruses pose a continued threat, not only to avian species but also to humans. We studied the pathogenicity of four human and nine avian H5N1/04 influenza viruses in ferrets (an excellent model for influenza studies). All four human isolates were fatal to intranasally inoculated ferrets. The human isolate A/Vietnam/1203/04 (H5N1) was the most pathogenic isolate; the severity of disease was associated with a broad tissue tropism and high virus titers in multiple organs, including the brain. High fever, weight loss, anorexia, extreme lethargy, and diarrhea were observed. Two avian H5N1/04 isolates were as pathogenic as the human viruses, causing lethal systemic infections in ferrets. Seven of nine H5N1/04 viruses isolated from avian species caused mild infections, with virus replication restricted to the upper respiratory tract. All chicken isolates were nonlethal to ferrets. A sequence analysis revealed polybasic amino acids in the hemagglutinin connecting peptides of all H5N1/04 viruses, indicating that multiple molecular differences in other genes are important for a high level of virulence. Interestingly, the human A/Vietnam/1203/04 isolate had a lysine substitution at position 627 of PB2 and had one to eight amino acid changes in all gene products except that of the M1 gene, unlike the A/chicken/Vietnam/C58/04 and A/quail/Vietnam/36/04 viruses. Our results indicate that viruses that are lethal to mammals are circulating among birds in Asia and suggest that pathogenicity in ferrets, and perhaps humans, reflects a complex combination of different residues rather than a single amino acid difference.
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Cites
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Cited by
Fatal outcome of human influenza A (H5N1) is associated with high viral load and hypercytokinemia.
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Avian Influenza Virus (H5N1): a Threat to Human Health
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Self-citationStructure and Receptor Specificity of the Hemagglutinin from an H5N1 Influenza Virus
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Pathogenesis and Transmission of Swine-Origin 2009 A(H1N1) Influenza Virus in Ferrets
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Metadata
- Authors
- Govorkova, E. A., Rehg, J. E., Krauss, S., Yen, H.-L., Guan, Y., Peiris, M., Nguyen, T. D., Hanh, T. H., Puthavathana, P., Long, H. T., Buranathai, C., Lim, W., Webster, R. G., & Hoffmann, E.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2005-02-15
- Volume
- 79
- Issue
- 4
- Pages
- 2191-2198
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2150054867
- Cited by
- 324
- References
- 37
- FWCI
- 27.05
- Type
- journal article
- Language
- en
- Open access
- green
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- Govorkova, E. A., Rehg, J. E., Krauss, S., Yen, H.-L., Guan, Y., Peiris, M., Nguyen, T. D., Hanh, T. H., Puthavathana, P., Long, H. T., Buranathai, C., Lim, W., Webster, R. G., & Hoffmann, E. (2005). Lethality to Ferrets of H5N1 Influenza Viruses Isolated from Humans and Poultry in 2004. Journal of Virology. https://doi.org/10.1128/jvi.79.4.2191-2198.2005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
