The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Species-Specific Variation in the B30.2(SPRY) Domain of TRIM5α Determines the Potency of Human Immunodeficiency Virus Restriction
Open accessAbstract
Retroviruses encounter dominant postentry restrictions in cells of particular species. Human immunodeficiency virus type 1 (HIV-1) is blocked in the cells of Old World monkeys by TRIM5α, a tripartite motif (TRIM) protein composed of RING, B-box 2, coiled-coil, and B30.2(SPRY) domains. Rhesus monkey TRIM5α (TRIM5α rh ) more potently blocks HIV-1 infection than human TRIM5α (TRIM5α hu ). Here, by studying chimeric TRIM5α proteins, we demonstrate that the major determinant of anti-HIV-1 potency is the B30.2(SPRY) domain. Analysis of species-specific variation in TRIM5α has identified three variable regions (v1, v2, and v3) within the B30.2 domain. The TRIM5α proteins of Old World primates exhibit expansion, duplication, and residue variation specifically in the v1 region. Replacement of three amino acids in the N terminus of the TRIM5α hu B30.2 v1 region with the corresponding TRIM5α rh residues resulted in a TRIM5α molecule that restricted HIV-1 nearly as efficiently as wild-type TRIM5α rh . Surprisingly, a single-amino-acid change in this region of TRIM5α hu allowed potent restriction of simian immunodeficiency virus, a phenotype not observed for either wild-type TRIM5α hu or TRIM5α rh . Some of the chimeric TRIM5α proteins that are >98% identical to the human protein yet mediate a strong restriction of HIV-1 infection may have therapeutic utility. These observations implicate the v1 variable region of the B30.2(SPRY) domain in TRIM5α rh antiviral potency.
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Barré-Sinoussi, F., Chermann, J. C., Rey, F., Nugeyre, M. T., Chamaret, S., Gruest, J., Dauguet, C., Axler-Blin, C., Vézinet-Brun, F., Rouzioux, C., et al. (1983) · Science · doi:10.1126/science.6189183 · Cited by 7,038
Gallo, R. C., Salahuddin, S. Z., Popovic, M., Shearer, G. M., Kaplan, M., Haynes, B. F., Palker, T. J., Redfield, R., Oleske, J., Safai, B., et al. (1984) · Science · doi:10.1126/science.6200936 · Cited by 3,641
The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys
Stremlau, M., Owens, C. M., Perron, M. J., Kiessling, M., Autissier, P., & Sodroski, J. (2004) · Nature · doi:10.1038/nature02343 · Cited by 1,816
Self-citationThe tripartite motif family identifies cell compartments
Reymond, A., Meroni, G., Fantozzi, A., Merla, G., Cairo, S., Luzi, L., Riganelli, D., Zanaria, E., Messali, S., Cainarca, S., et al. (2001) · The EMBO Journal · doi:10.1093/emboj/20.9.2140 · Cited by 1,366
Isolation of T-Cell Tropic HTLV-III-Like Retrovirus from Macaques
Daniel, M. D., Letvin, N. L., King, N. W., Kannagi, M., Sehgal, P. K., Hunt, R. D., Kanki, P. J., Essex, M., & Desrosiers, R. C. (1985) · Science · doi:10.1126/science.3159089 · Cited by 920
HIV-1 Gag proteins: diverse functions in the virus life cycle
Freed, E. O. (1998) · Virology · doi:10.1006/viro.1998.9398 · Cited by 717
Cyclophilin A retrotransposition into TRIM5 explains owl monkey resistance to HIV-1
Sayah, D. M., Sokolskaja, E., Berthoux, L., & Luban, J. (2004) · Nature · doi:10.1038/nature02777 · Cited by 662
Induction of AIDS-Like Disease in Macaque Monkeys with T-Cell Tropic Retrovirus STLV-III
Letvin, N. L., Daniel, M. D., Sehgal, P. K., Desrosiers, R. C., Hunt, R. D., Waldron, L. M., MacKey, J. J., Schmidt, D. K., Chalifoux, L. V., & King, N. W. (1985) · Science · doi:10.1126/science.2412295 · Cited by 616
Trim5α protein restricts both HIV-1 and murine leukemia virus
Yap, M. W., Nisole, S., Lynch, C., & Stoye, J. P. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402876101 · Cited by 455
Cyclophilin A modulates the sensitivity of HIV-1 to host restriction factors
Towers, G. J., Hatziioannou, T., Cowan, S., Goff, S. P., Luban, J., & Bieniasz, P. D. (2003) · Nature Medicine · doi:10.1038/nm910 · Cited by 377
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TRIM family proteins: retroviral restriction and antiviral defence
Nisole, S., Stoye, J. P., & Saïb, A. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1248 · Cited by 761
Rules of engagement: molecular insights from host–virus arms races
Daugherty, M. D., & Malik, H. S. (2012) · Annual Review of Genetics · doi:10.1146/annurev-genet-110711-155522 · Cited by 515
Restriction of HIV-1 and other retroviruses by TRIM5
Ganser-Pornillos, B. K., & Pornillos, O. (2019) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0225-2 · Cited by 163
Restriction of an Extinct Retrovirus by the Human TRIM5α Antiviral Protein
Kaiser, S. M., Malik, H. S., & Emerman, M. (2007) · Science · doi:10.1126/science.1140579 · Cited by 145
Two-stepping through time: mammals and viruses
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Metadata
- Authors
- Stremlau, M., Perron, M., Welikala, S., & Sodroski, J.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2005-03
- Volume
- 79
- Issue
- 5
- Pages
- 3139-3145
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2097660625
- Cited by
- 373
- References
- 35
- FWCI
- 11.88
- Type
- journal article
- Language
- en
- Open access
- green
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- Stremlau, M., Perron, M., Welikala, S., & Sodroski, J. (2005). Species-Specific Variation in the B30.2(SPRY) Domain of TRIM5α Determines the Potency of Human Immunodeficiency Virus Restriction. Journal of Virology. https://doi.org/10.1128/jvi.79.5.3139-3145.2005
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