Cited by 386Oral microbiology and periodontitis researchGut microbiota and healthGenomics and Phylogenetic Studies

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Metatranscriptomics of the Human Oral Microbiome during Health and Disease

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Jorth, Peter, Turner, Keith H., Gumus, Pinar, Nizam, Nejat, Buduneli, Nurcan, Whiteley, Marvin·2014·mBio

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Abstract

The human microbiome plays important roles in health, but when disrupted, these same indigenous microbes can cause disease. The composition of the microbiome changes during the transition from health to disease; however, these changes are often not conserved among patients. Since microbiome-associated diseases like periodontitis cause similar patient symptoms despite interpatient variability in microbial community composition, we hypothesized that human-associated microbial communities undergo conserved changes in metabolism during disease. Here, we used patient-matched healthy and diseased samples to compare gene expression of 160,000 genes in healthy and diseased periodontal communities. We show that health- and disease-associated communities exhibit defined differences in metabolism that are conserved between patients. In contrast, the metabolic gene expression of individual species was highly variable between patients. These results demonstrate that despite high interpatient variability in microbial composition, disease-associated communities display conserved metabolic profiles that are generally accomplished by a patient-specific cohort of microbes. IMPORTANCE The human microbiome project has shown that shifts in our microbiota are associated with many diseases, including obesity, Crohn’s disease, diabetes, and periodontitis. While changes in microbial populations are apparent during these diseases, the species associated with each disease can vary from patient to patient. Taking into account this interpatient variability, we hypothesized that specific microbiota-associated diseases would be marked by conserved microbial community behaviors. Here, we use gene expression analyses of patient-matched healthy and diseased human periodontal plaque to show that microbial communities have highly conserved metabolic gene expression profiles, whereas individual species within the community do not. Furthermore, disease-associated communities exhibit conserved changes in metabolic and virulence gene expression.

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Background
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Influential
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Background
Neutrophil Interaction with Emerging Oral Pathogens: A Novel View of the Disease Paradigm
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Method
Elevated Dietary Carbohydrate and Glycemic Intake Associate with an Altered Oral Microbial Ecosystem in Two Large U.S. Cohorts
Monson, K. R., Peters, B. A., Usyk, M., Um, C. Y., Oberstein, P. E., McCullough, M. L., Purdue, M. P., Freedman, N. D., Hayes, R. B., & Ahn, J. (2022) · Cancer Research Communications · doi:10.1158/2767-9764.crc-22-0323 · Cited by 10
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Microbial functional pathways based on metatranscriptomic profiling enable effective saliva-based health assessments for precision wellness
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Background
Quantifying synthetic bacterial community composition with flow cytometry: efficacy in mock communities and challenges in co-cultures
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Application of Metagenomic Analyses in Dentistry as a Novel Strategy Enabling Complex Insight into Microbial Diversity of the Oral Cavity
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Is Fluorescence Technology a Promising Tool for Detecting Infected Dentin in Deep Carious Lesions?
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Authors: Jorth, P., Turner, K. H., Gumus, P., Nizam, N., Buduneli, N., & Whiteley, M.
Affiliations: The University of Texas at Austin (US)
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey
Ege University (TR)
ORCID: Jorth, Peter
Nizam, Nejat
Buduneli, Nurcan
Whiteley, Marvin
Venue: mBio
Publisher: American Society for Microbiology
Published: 2014-05
Volume: 5
Issue: 2
Pages: e01012-14
ISSN: 2150-7511 (ISSN-L)
2161-2129
DOI: 10.1128/mbio.01012-14
PMC: PMC3977359
OpenAlex: W2146975236
Cited by: 386
References: 33
Citations by year: 2026: 18 · 2025: 30 · 2024: 24 · 2023: 13 · 2022: 25 · 2021: 33 · 2020: 45 · 2019: 39 · 2018: 33 · 2017: 40 · 2016: 33 · 2015: 39 · 2014: 14
FWCI: 56.09
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by-nc-sa
creativecommons.org/licenses/by-nc-sa/3.0/ (vor)
journals.asm.org/non-commercial-tdm-license (tdm)
Locations: mBio (publishedVersion, cc-by-nc-sa)
Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
PubMed Central (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders: National Institutes of Health (5f31de021633-02)
National Institute of Dental and Craniofacial Research (F31 DE021633, R01DE023193, 5F31DE021633-02, R01DE020100)
Topics: Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords: oral microbiota, periodontitis, metatranscriptomics, microbial community metabolism, interpatient variability, periodontal plaque, virulence gene expression
MeSH terms: Adult, Female, Humans, Male, Mouth, Periodontitis, Metagenome, Transcriptome, Microbiota, Dysbiosis

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Metatranscriptomics of the Human Oral Microbiome during Health and Disease

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