Cited by 386Oral microbiology and periodontitis researchGut microbiota and healthGenomics and Phylogenetic Studies

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Metatranscriptomics of the Human Oral Microbiome during Health and Disease

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Jorth, Peter, Turner, Keith H., Gumus, Pinar, Nizam, Nejat, Buduneli, Nurcan, Whiteley, Marvin·2014·mBio

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Abstract

The human microbiome plays important roles in health, but when disrupted, these same indigenous microbes can cause disease. The composition of the microbiome changes during the transition from health to disease; however, these changes are often not conserved among patients. Since microbiome-associated diseases like periodontitis cause similar patient symptoms despite interpatient variability in microbial community composition, we hypothesized that human-associated microbial communities undergo conserved changes in metabolism during disease. Here, we used patient-matched healthy and diseased samples to compare gene expression of 160,000 genes in healthy and diseased periodontal communities. We show that health- and disease-associated communities exhibit defined differences in metabolism that are conserved between patients. In contrast, the metabolic gene expression of individual species was highly variable between patients. These results demonstrate that despite high interpatient variability in microbial composition, disease-associated communities display conserved metabolic profiles that are generally accomplished by a patient-specific cohort of microbes. IMPORTANCE The human microbiome project has shown that shifts in our microbiota are associated with many diseases, including obesity, Crohn’s disease, diabetes, and periodontitis. While changes in microbial populations are apparent during these diseases, the species associated with each disease can vary from patient to patient. Taking into account this interpatient variability, we hypothesized that specific microbiota-associated diseases would be marked by conserved microbial community behaviors. Here, we use gene expression analyses of patient-matched healthy and diseased human periodontal plaque to show that microbial communities have highly conserved metabolic gene expression profiles, whereas individual species within the community do not. Furthermore, disease-associated communities exhibit conserved changes in metabolic and virulence gene expression.

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Defining functional signatures of dysbiosis in periodontitis progression
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Background
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BackgroundMethod
Periodontitis and diabetes mellitus co-morbidity: A molecular dialogue
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Background
Maintaining a protective state for human periodontal tissue
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Background
Metatranscriptomic analyses of the oral microbiome
Duran-Pinedo, A. E. (2020) · Periodontology 2000 · doi:10.1111/prd.12350 · Cited by 62
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Background
Oral microbiome in HIV-associated periodontitis
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Self-citation
Development and characterization of an oral microbiome transplant among Australians for the treatment of dental caries and periodontal disease: A study protocol
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Background
Microbial dysbiosis: The root cause of periodontal disease
Kumar, P. (2021) · Journal of Periodontology · doi:10.1002/jper.21-0245 · Cited by 64
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“…have focused on insect endosymbionts, which generally involve only a few bacterial species (Dale and Moran, 2006), whereas more recent studies, mainly in humans, have revealed a complex community of microbes coexisting with their host (Turnbaugh et al., 2007; Grice, 2014; Jorth et al., 2014).”
Background
Metabolic Signaling and Spatial Interactions in the Oral Polymicrobial Community
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“These studies suggest that the overall metabolic potential of the community may be more correlative with disease than the presence of individual species (Jorth et al. 2014).”
Background
Protein relative abundance patterns associated with sucrose-induced dysbiosis are conserved across taxonomically diverse oral microcosm biofilm models of dental caries
Rudney, J. D., Jagtap, P. D., Reilly, C., Chen, R., Markowski, T. W., Higgins, L., Johnson, J. E., & Griffin, T. J. (2015) · Microbiome · doi:10.1186/s40168-015-0136-z · Cited by 54
“They observed that taxonomically diverse diseased sites shared conserved gene expression profiles [21].”
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Deng, Z.-L., Sztajer, H., Jarek, M., Bhuju, S., & Wagner‐Döbler, I. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00124 · Cited by 55
“Jorth et al. (2014) compared microbial communities in healthy and diseased periodontal pockets in the same individual; they suggested that although the species composition in periodontal pockets varies widely, the metabolic networks operating in disease are conserved.”
Background
Managing Oral Health in the Context of Antimicrobial Resistance
Bessa, L. J., Botelho, J., Machado, V., Alves, R. C., & Mendes, J. J. (2022) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph192416448 · Cited by 57
“Despite the interpatient variability in microbial composition between healthy individuals and diseased patients, disease-associated microbial communities display conserved metabolic profiles that are generally accomplished by a patient-specific microbiome [36].”
Background
Utilizing de Bruijn graph of metagenome assembly for metatranscriptome analysis
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Method
Whole metagenomic shotgun sequencing of the subgingival microbiome of diabetics and non-diabetics with different periodontal conditions
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Lafaurie, G. I., Neuta, Y., Ríos, R., Pacheco, M., Pianeta, R., Castillo, D. M., Herrera, D., Reyes, J., Díaz, L., Castillo, Y., et al. (2022) · PLoS ONE · doi:10.1371/journal.pone.0273523 · Cited by 49
“Although a greater alpha diversity of the oral microbiome was associated with periodontal health [30] or gingivitis [28,31] compared with periodontitis, other studies have reported greater diversity in periodontitis [14,32] or have not found differences [33].”
Result
Microbial diagnostics in periodontal diseases
Manoil, D., Parga, A., Bostancı, N., & Belibasakis, G. N. (2024) · Periodontology 2000 · doi:10.1111/prd.12571 · Cited by 51
A Meta-Transcriptomics Survey Reveals Changes in the Microbiota of the Chinese Mitten Crab Eriocheir sinensis Infected with Hepatopancreatic Necrosis Disease
Shen, H., Zang, Y., Song, K., Ma, Y., Dai, T., & Serwadda, A. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00732 · Cited by 48
“The evolutionarily-stable commensal microbes positively and negatively affect host health, and when this balance is disrupted, symbiotic microbes can induce disease (Breznak and Brune, 1994; Douglas, 1998; Fraune and Zimmer, 2008; Jorth et al., 2014).”
Background
Correction: Salivary inflammatory markers and microbiome in normoglycemic lean and obese children compared to obese children with type 2 diabetes
Janem, W. F., Scannapieco, F. A., Sabharwal, A., Tsompana, M., Berman, H. A., Haase, E. M., Miecznikowski, J. C., & Mastrandrea, L. D. (2017) · PLoS ONE · doi:10.1371/journal.pone.0183600 · Cited by 48
Metagenomic and Metatranscriptomic Insight Into Oral Biofilms in Periodontitis and Related Systemic Diseases
Huang, Y., Zhao, X., Cui, L., & Huang, S. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.728585 · Cited by 50
Complement therapeutics in inflammatory diseases: promising drug candidates for C3‐targeted intervention
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“caused by a single or a limited number of pathogens, but rather is the result of a polymicrobial community-induced disruption of the host’s homeostasis leading to destructive inflammation in susceptible individuals (Kumar et al., 2006; Dewhirst et al., 2010; Hajishengallis et al., 2011; Griffen et al., 2012; Hajisheng…”
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“Dietary [5] and xenobiotic treatments [6] have been found to alter significantly gut microbial gene expression profiles, while health-and disease-associated oral microbiota communities displayed defined metabolic differences [7].”
Background
Polymicrobial Interactions of Oral Microbiota: a Historical Review and Current Perspective
Zhang, M., Whiteley, M., & Lewin, G. R. (2022) · mBio · doi:10.1128/mbio.00235-22 · Cited by 50
“nucleatum does not significantly change in relative abundance between healthy and diseased samples, but metatranscriptomics shows that its metabolism is altered (40).”
BackgroundSelf-citation
Effects of altitude on human oral microbes
Fang, L., Liang, T., Zhang, Z., Liu, L., Li, J., Dong, W., Zhang, H., Bai, S., Ma, L., & Kang, L. (2021) · AMB Express · doi:10.1186/s13568-021-01200-0 · Cited by 48
“Disorders of the oral microbiota are associated with various diseases, such as dental caries, periodontitis, peri-implantitis, mucosal diseases, and oral cancers (Jorth et al. 2014; Gao et al. 2018; Wasfi et al. 2018).”
Background
Quorum sensing of Streptococcus mutans is activated by Aggregatibacter actinomycetemcomitans and by the periodontal microbiome
Szafrański, S. P., Deng, Z.-L., Tomasch, J., Jarek, M., Bhuju, S., Rohde, M., Sztajer, H., & Wagner‐Döbler, I. (2017) · BMC Genomics · doi:10.1186/s12864-017-3618-5 · Cited by 44
Metabolic network percolation quantifies biosynthetic capabilities across the human oral microbiome
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“The organic acids were chosen by searching through the literature for those that were found to be relevant for oral health ( Jorth et al., 2014 ; Takahashi, 2015 ).”
BackgroundMethod
Diversity and Biogeography of Human Oral Saliva Microbial Communities Revealed by the Earth Microbiome Project
Wang, J., Feng, J., Zhu, Y., Li, D., Wang, J., & Chi, W. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.931065 · Cited by 46
Population-Genomic Insights into Variation in Prevotella intermedia and Prevotella nigrescens Isolates and Its Association with Periodontal Disease
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“Some studies have shown differences in bacterial composition and gene expression between periodontally healthy and diseased sites by using metatranscriptomic sequencing (19, 20).”
Background
Deciphering the human microbiome using next-generation sequencing data and bioinformatics approaches
Kim, Y., Koh, I., & Rho, M. (2014) · Methods · doi:10.1016/j.ymeth.2014.10.022 · Cited by 44
Association of Coffee and Tea Intake with the Oral Microbiome: Results from a Large Cross-Sectional Study
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“Specifically in regards to the oralmicrobiota, oral disease states (caries, periodontal disease) have been associated with reduced oral microbiome diversity (44, 45), suggesting that tea promotes a healthy oral microbiome.”
Background
Using Bacterial Transcriptomics to Investigate Targets of Host-Bacterial Interactions in Caenorhabditis elegans
Chan, J., Wright, J., Wong, H. T., Ardasheva, A., Brumbaugh, J. T., McLimans, C. J., & Lamendella, R. (2019) · Scientific Reports · doi:10.1038/s41598-019-41452-2 · Cited by 39
The paradox of painless periodontal disease
Gaurilcikaite, E., Renton, T. F., & Grant, A. D. (2016) · Oral Diseases · doi:10.1111/odi.12537 · Cited by 40
Microbiology of Apical Periodontitis
Siqueira, J. F., Jr., & Rôças, I. N. (2019) · Wiley eBooks · doi:10.1002/9781119272014.ch4 · Cited by 39
Microbial Community Composition Impacts Pathogen Iron Availability during Polymicrobial Infection
Stacy, A., Abraham, N., Jorth, P. A., & Whiteley, M. (2016) · PLoS Pathogens · doi:10.1371/journal.ppat.1006084 · Cited by 43
Self-citation
Localized aggressive periodontitis immune response to healthy and diseased subgingival plaque
Shaddox, L. M., Spencer, W. P., Velsko, I. M., Al‐Kassab, H., Huang, H., Calderon, N., Aukhil, I., & Wallet, S. M. (2016) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12560 · Cited by 38
“These findings indicate that high species diversity may be a more important factor in disease development and progression, which is in contrast to chronic periodontal disease, in which there appears to be a decrease in species diversity (Jorth et al., 2014, Socransky and Haffajee, 2005).”
Background
Biofilm ecology associated with dental caries: understanding of microbial interactions in oral communities leads to development of therapeutic strategies targeting cariogenic biofilms
Cai, J.-N., & Kim, D. (2023) · Advances in applied microbiology · doi:10.1016/bs.aambs.2023.02.001 · Cited by 43
A Graph-Centric Approach for Metagenome-Guided Peptide and Protein Identification in Metaproteomics
Tang, H., Li, S., & Ye, Y. (2016) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1005224 · Cited by 37
“Current metatranscriptomic and metaproteomic studies often directly adopt protocols originally developed for the transcriptomic and proteomic studies of model bacterial species; for examples, many metatranscriptomic projects exploited the bacterial RNA-seq protocol [17, 18], while most metaproteomic studies applied th…”
Method
From Mouth to Model: Combining in vivo and in vitro Oral Biofilm Growth
Klug, B., Santigli, E., Westendorf, C., Tangl, S., Wimmer, G., & Grube, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01448 · Cited by 36
Gut region influences the diversity and interactions of bacterial communities in pikas (Ochotona curzoniae and Ochotona daurica)
Li, H., Li, T., Berasategui, A., Rui, J., Zhang, X., Li, C., Xiao, Z., & Li, X. (2017) · FEMS Microbiology Ecology · doi:10.1093/femsec/fix149 · Cited by 36
Microbiome Profiles of Ligature-Induced Periodontitis in Nonhuman Primates across the Life Span
Kirakodu, S. S., Chen, J., González‐Martínez, J., González, O. A., & Ebersole, J. L. (2019) · Infection and Immunity · doi:10.1128/iai.00067-19 · Cited by 36
Behavioral strategies for periodontal health
Suvan, J. E., Sabalić, M., Araújo, M., & Ramseier, C. A. (2022) · Periodontology 2000 · doi:10.1111/prd.12462 · Cited by 50
Illuminating the oral microbiome and its host interactions: recent advancements in omics and bioinformatics technologies in the context of oral microbiome research
Baker, J. L. (2023) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuad051 · Cited by 37
Strand-specific community RNA-seq reveals prevalent and dynamic antisense transcription in human gut microbiota
Bao, G., Wang, M., Doak, T. G., & Ye, Y. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00896 · Cited by 34
“Current analyses of metatranscriptomic datasets focus on the detection of gene expression levels and the study of the relationship between changes of gene expression and changes of environment.”
Background
Widespread, human-associated redondoviruses infect the commensal protozoan Entamoeba gingivalis
Keeler, E. L., Merenstein, C., Reddy, S. G., Taylor, L. J., Güemes, A. G. C., Zankharia, U. S., Collman, R. G., & Bushman, F. D. (2022) · Cell Host & Microbe · doi:10.1016/j.chom.2022.11.002 · Cited by 34
Multi‐Omics Signatures of Periodontitis and Periodontal Therapy on the Oral and Gut Microbiome
Baima, G., Dabdoub, S. M., Thumbigere‐Math, V., Ribaldone, D. G., Caviglia, G. P., Tenori, L., Fantato, L., Vignoli, A., Romandini, M., Ferrocino, I., et al. (2025) · Journal of Periodontal Research · doi:10.1111/jre.70055 · Cited by 34
Mechanisms underlying interactions between two abundant oral commensal bacteria
Perera, D., McLean, A. R., Morillo-López, V., Cloutier-Leblanc, K., Almeida, E., Cabana, K., Welch, J. L. M., & Ramsey, M. M. (2021) · The ISME Journal · doi:10.1038/s41396-021-01141-3 · Cited by 33
“parainfluenzae gene expression in published metatranscriptome datasets [59, 60].”
Background
Re-discovering periodontal butyric acid: New insights on an old metabolite
Cueno, M. E., & Ochiai, K. (2015) · Microbial Pathogenesis · doi:10.1016/j.micpath.2015.10.006 · Cited by 34
Clinical and microbiological parameters of naturally occurring periodontitis in the non-human primate Macaca mulatta
Colombo, A. P. V., Paster, B. J., Grimaldi, G., Lourenço, T. G. B., Têva, A., Campos‐Neto, A., McCluskey, J., Kleanthous, H., Dyke, T. E. V., & Stashenko, P. (2017) · Journal of Oral Microbiology · doi:10.1080/20002297.2017.1403843 · Cited by 33
Studying the Human Microbiota
Walker, A. W. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-31248-4_2 · Cited by 32
A survey on predicting microbe-disease associations: biological data and computational methods
Wen, Z., Yan, C., Duan, G., Li, S., Wu, F., & Wang, J. (2020) · Briefings in Bioinformatics · doi:10.1093/bib/bbaa157 · Cited by 31
Oral Microbiome and Gingival Transcriptome Profiles of Ligature-Induced Periodontitis
Ebersole, J., Kirakodu, S., Chen, J., Nagarajan, R., & Gonzalez, O. (2020) · Journal of dentistry research · doi:10.1177/0022034520906138 · Cited by 26
“While the microbiomes in health and disease have been described in humans (Wade 2013; Jorth et al. 2014; Feres et al. 2016; Kilian et al. 2016), limitations of studies of human disease do not allow definitive interpretation of how early in an active disease process critical microbiome alterations occur, as well as the…”
Background
Obligate anaerobes are abundant in human necrotizing soft tissue infection samples – a metagenomics analysis
Zhao-Fleming, H. H., Wilkinson, J. E., Larumbe‐Zabala, E., Dissanaike, S., & Rumbaugh, K. P. (2019) · Apmis · doi:10.1111/apm.12969 · Cited by 29
“DNA was extracted via ethanol precipitation, following an established protocol (16).”
Method
Distinct Regulatory Role of Carbon Catabolite Protein A (CcpA) in Oral Streptococcal spxB Expression
Redanz, S., Masilamani, R., Cullin, N., Giacaman, R. A., Merritt, J. L., & Kreth, J. (2018) · Journal of Bacteriology · doi:10.1128/jb.00619-17 · Cited by 27
“oralis in two out of three patients with generalized aggressive periodontitis (44).”
Background
Nonulosonic acids contribute to the pathogenicity of the oral bacterium Tannerella forsythia
Bloch, S., Tomek, M. B., Friedrich, V., Messner, P., & Schäffer, C. (2019) · Interface Focus · doi:10.1098/rsfs.2018.0064 · Cited by 28
Characterization of Bacterial Community Dynamics of the Human Mouth Throughout Decomposition via Metagenomic, Metatranscriptomic, and Culturing Techniques
Ashe, E. C., Comeau, A., Zejdlik, K. J., & O’Connell, S. P. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.689493 · Cited by 30
Consistent and reproducible long-term in vitro growth of health and disease-associated oral subgingival biofilms
Velsko, I. M., & Shaddox, L. M. (2018) · BMC Microbiology · doi:10.1186/s12866-018-1212-x · Cited by 30
“biofilm plaque from healthy and periodontitis-affected sites suggest that community activity is more uniform across disease sites than is microbial profile [6, 7, 55], and further investigation of protein expression and metabolic output in healthy and disease-site derived biofilms is warranted [56].”
Background
Comparative Metatranscriptomics of Periodontitis Supports a Common Polymicrobial Shift in Metabolic Function and Identifies Novel Putative Disease-Associated ncRNAs
Ram-Mohan, N., & Meyer, M. M. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00482 · Cited by 27
Omics and interspecies interaction
Shokeen, B., Dinis, M., Haghighi, F., Tran, N. C., & Lux, R. (2020) · Periodontology 2000 · doi:10.1111/prd.12354 · Cited by 28
Porphyromonas spp., Fusobacterium spp., and Bacteroides spp. dominate microbiota in the course of macropod progressive periodontal disease
Yip, S., Dehcheshmeh, M. M., McLelland, D. J., Boardman, W. S. J., Saputra, S., Ebrahimie, E., Weyrich, L. S., Bird, P. S., & Trott, D. J. (2021) · Scientific Reports · doi:10.1038/s41598-021-97057-1 · Cited by 32
Can the microbiome influence host evolutionary trajectories?Preprint
Henry, L. P., Bruijning, M., Forsberg, S. K. G., & Ayroles, J. F. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/700237 · Cited by 27
World Workshop on Oral Medicine VII: Targeting the oral microbiome Part 2: Current knowledge on malignant and potentially malignant oral disorders
Robledo‐Sierra, J., Amy, D. P. B., Varoni, E. M., Bavarian, R., Simonsen, J. L., Paster, B. J., Wade, W. G., Kerr, A. R., Peterson, D. E., & Lau, E. F. (2019) · Oral Diseases · doi:10.1111/odi.13107 · Cited by 26
“Likewise, a study using metatranscriptomics reported that although there was highly variable metabolic gene expression of the individual species, the metabolism of the entire microbial community associated with periodontal disease was similar among periodontitis patients (Jorth et al., 2014).”
Background
Advances, practice, and clinical perspectives in high‐throughput sequencing
Park, S., Saito-Adachi, M., Komiyama, Y., & Nakai, K. (2015) · Oral Diseases · doi:10.1111/odi.12403 · Cited by 26
“Human oral microorganisms are increasingly important for studying oral pathogens (Jorth et al, 2014; Xu and Gunsolley, 2014; Duran-Pinedo and Frias-Lopez, 2015).”
Background
Could Periodontal Disease through Periopathogen Fusobacterium nucleatum Be an Aggravating Factor for Gastric Cancer?
Șurlin, P., Nicolae, F. M., Şurlin, V., Pătrașcu, Ș., Ungureanu, B. S., Didilescu, A. C., & Gheonea, D. I. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9123885 · Cited by 25
“Fusobacterium nucleatum upregulates the synthesis of butyrate, which is a cytotoxic fatty acid involved in periodontal disease, especially in aggressive periodontitis [52,53].”
Background
metaModules identifies key functional subnetworks in microbiome-related disease
May, A., Brandt, B. W., El-Kebir, M., Klau, G. W., Zaura, E., Crielaard, W., Heringa, J., & Abeln, S. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btv526 · Cited by 24
“nucleatum species was proposed to be responsible for butanoate production (Jorth et al., 2014). Our analysis suggests otherwise; a wider range of organisms seems responsible for the production of butanoate. Note that our reference metagenome database included genes from all species in KEGG, while Jorth et al. (2014) u…”
Background
Proteomic analysis of Fusobacterium nucleatum growth in biofilm versus planktonic state
Llama‐Palacios, A., Potupa, O., Sánchez, M. C., Figuero, E., Herrera, D., & Sanz, M. (2020) · Molecular Oral Microbiology · doi:10.1111/omi.12303 · Cited by 25
New Technologies for Studying Biofilms
Franklin, M. J., Chang, C. B., Akiyama, T., & Bothner, B. B. (2015) · ASM Press eBooks · doi:10.1128/9781555817466.ch1 · Cited by 24
Microbiome based precision medicine through integrated multiomics and machine learning
Kumar, A., Xu, C., & Dakal, T. C. (2025) · Microbiological Research · doi:10.1016/j.micres.2025.128384 · Cited by 24
A quantitative framework reveals traditional laboratory growth is a highly accurate model of human oral infection
Lewin, G. R., Stocke, K. S., Lamont, R. J., & Whiteley, M. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2116637119 · Cited by 23
Self-citation
Colorectal Tumour Mucosa Microbiome Is Enriched in Oral Pathogens and Defines Three Subtypes That Correlate with Markers of Tumour Progression
Zwinsová, B., Петров, В. А., Hrivňáková, M., Smatana, S., Micenková, L., Kazdová, N., Popovici, V. C., Hrstka, R., Šefr, R., Bencsiková, B., et al. (2021) · Cancers · doi:10.3390/cancers13194799 · Cited by 23
Oral microbiota–host interaction: the chief culprit of alveolar bone resorption
Xu, J., Yu, L., Ye, S., Ye, Z., Yang, L., & Xu, X. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1254516 · Cited by 22
Microbiota in Human Periodontal Abscess Revealed by 16S rDNA Sequencing
Chen, J., Wu, X., Zhu, D., Xu, M., Yu, Y., Yu, L., & Zhang, W. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01723 · Cited by 22
Grade C molar-incisor pattern periodontitis subgingival microbial profile before and after treatment
Velsko, I. M., Harrison, P., Chalmers, N. I., Barb, J. J., Huang, H., Aukhil, I., & Shaddox, L. M. (2020) · Journal of Oral Microbiology · doi:10.1080/20002297.2020.1814674 · Cited by 22
“[46] reported slightly different results in grade C periodontitis sites.”
Result
An infection of Enterobacter ludwigii affects development and causes age-dependent neurodegeneration in Drosophila melanogaster
Priyadarsini, S., Moumita, S., Sahu, S., Jayabalan, R., & Mishra, M. (2019) · Invertebrate Neuroscience · doi:10.1007/s10158-019-0233-y · Cited by 22
“The gut microbiome population varies from person to person, region to region, depending on the diet (Jorth et al. 2014).”
Background
Exploring the Role of IL-17A in Oral Dysbiosis-Associated Periodontitis and Its Correlation with Systemic Inflammatory Disease
Irie, K., Azuma, T., Tomofuji, T., & Yamamoto, T. (2023) · Dentistry Journal · doi:10.3390/dj11080194 · Cited by 24
“Interestingly, dysbiotic communities were less diverse, but more similar among each other than healthy periodontal pocket communities [52].”
Background
The Oral Microbiome in Health and Disease
Olsen, I. (2015) · Springer eBooks · doi:10.1007/978-3-319-25091-5_10 · Cited by 21
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Oral microbiota in xerostomia patients - A preliminary study
Weng, C.-T., Huang, S., Yang, H.-W., Kao, C., Wei, C.-C., & Huang, Y. (2021) · Journal of Dental Sciences · doi:10.1016/j.jds.2021.08.007 · Cited by 21
“The microbial population, different from normal constitution, are apparent in several pathologic conditions.(7) Thus, we hypothesized that a change in the amount of saliva will alter the composition of oral microbiota.”
Background
Specifically targeted antimicrobial peptides: A new and promising avenue in selective antimicrobial therapy
Medhi, B., Sarma, P., Mahendiratta, S., & Prakash, A. (2018) · Indian Journal of Pharmacology · doi:10.4103/ijp.ijp_218_18 · Cited by 21
“Data from human microbiome project have shown that alteration in the normal microbiota is associated with many diseases, including obesity, periodontitis, inflammatory bowel disease, irritable bowel syndrome, diabetes, cancer, rheumatoid arthritis, Alzheimer’s disease, and Parkinsonism’s disease.[2,5] Alteration of th…”
Background
Metatranscriptome and Resistome of the Endodontic Microbiome
Pinheiro, E. T., Karygianni, L., Candeiro, G. T. D. M., Vilela, B. G., Dantas, L. O., Pereira, A. C. C., Gomes, B. P. F. A., Attin, T., Thurnheer, T., & Russo, G. (2024) · Journal of Endodontics · doi:10.1016/j.joen.2024.03.015 · Cited by 20
Metatranscriptomic Analysis Reveals an Imbalance of Hepatopancreatic Flora of Chinese Mitten Crab Eriocheir sinensis with Hepatopancreatic Necrosis Disease
Shen, Z., Kumar, D., Liu, X., Yan, B., Fang, P., Gu, Y., Li, M., Xie, M., Yuan, R., Feng, Y., et al. (2021) · Biology · doi:10.3390/biology10060462 · Cited by 19
“Host disease can be induced by symbiotic microbes after this balance is disrupted [43,45].”
Background
A prospective interventional trial on the effect of periodontal treatment on Fusobacterium nucleatum abundance in patients with colorectal tumours
Yoshihara, T., Kioi, M., Baba, J., Usuda, H., Kessoku, T., Iwaki, M., Takatsu, T., Misawa, N., Ashikari, K., Matsuura, T., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-03083-4 · Cited by 21

Authors: Jorth, P., Turner, K. H., Gumus, P., Nizam, N., Buduneli, N., & Whiteley, M.
Affiliations: The University of Texas at Austin (US)
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey
Ege University (TR)
ORCID: Jorth, Peter
Nizam, Nejat
Buduneli, Nurcan
Whiteley, Marvin
Venue: mBio
Publisher: American Society for Microbiology
Published: 2014-05
Volume: 5
Issue: 2
Pages: e01012-14
ISSN: 2150-7511 (ISSN-L)
2161-2129
DOI: 10.1128/mbio.01012-14
PMC: PMC3977359
OpenAlex: W2146975236
Cited by: 386
References: 33
Citations by year: 2026: 18 · 2025: 30 · 2024: 24 · 2023: 13 · 2022: 25 · 2021: 33 · 2020: 45 · 2019: 39 · 2018: 33 · 2017: 40 · 2016: 33 · 2015: 39 · 2014: 14
FWCI: 56.09
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by-nc-sa
creativecommons.org/licenses/by-nc-sa/3.0/ (vor)
journals.asm.org/non-commercial-tdm-license (tdm)
Locations: mBio (publishedVersion, cc-by-nc-sa)
Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
PubMed Central (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders: National Institutes of Health (5f31de021633-02)
National Institute of Dental and Craniofacial Research (F31 DE021633, R01DE023193, 5F31DE021633-02, R01DE020100)
Topics: Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords: oral microbiota, periodontitis, metatranscriptomics, microbial community metabolism, interpatient variability, periodontal plaque, virulence gene expression
MeSH terms: Adult, Female, Humans, Male, Mouth, Periodontitis, Metagenome, Transcriptome, Microbiota, Dysbiosis

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Metatranscriptomics of the Human Oral Microbiome during Health and Disease

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