The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Selective Anchoring of DNA Methyltransferases 3A and 3B to Nucleosomes Containing Methylated DNA
Open accessAbstract
Proper DNA methylation patterns are essential for mammalian development and differentiation. DNA methyltransferases (DNMTs) primarily establish and maintain global DNA methylation patterns; however, the molecular mechanisms for the generation and inheritance of methylation patterns are still poorly understood. We used sucrose density gradients of nucleosomes prepared by partial and maximum micrococcal nuclease digestion, coupled with Western blot analysis to probe for the interactions between DNMTs and native nucleosomes. This method allows for analysis of the in vivo interactions between the chromatin modification enzymes and their actual nucleosomal substrates in the native state. We show that little free DNA methyltransferase 3A and 3B (DNMT3A/3B) exist in the nucleus and that almost all of the cellular contents of DNMT3A/3B, but not DNMT1, are strongly anchored to a subset of nucleosomes. This binding of DNMT3A/3B does not require the presence of other well-known chromatin-modifying enzymes or proteins, such as proliferating cell nuclear antigen, heterochromatin protein 1, methyl-CpG binding protein 2, Enhancer of Zeste homolog 2, histone deacetylase 1, and UHRF1, but it does require an intact nucleosomal structure. We also show that nucleosomes containing methylated SINE and LINE elements and CpG islands are the main sites of DNMT3A/3B binding. These data suggest that inheritance of DNA methylation requires cues from the chromatin component in addition to hemimethylation.
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Targeted mutation of the DNA methyltransferase gene results in embryonic lethality
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Epigenetics in human disease and prospects for epigenetic therapy
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Self-citationGenomic DNA methylation: the mark and its mediators.
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“(61), who also found DNMT3A to be enriched on six different methylated CGIs and DNMT3B occupying a subset of these methylated CGIs bound by DNMT3A.”
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Cited by
A decade of exploring the cancer epigenome—biological and translational implications
Baylin, S. B., & Jones, P. A. (2011) · Nature Reviews Cancer · doi:10.1038/nrc3130 · Cited by 2,871
Self-citationGenomic profiling of DNA methyltransferases reveals a role for DNMT3B in genic methylation
Baubec, T., Colombo, D. F., Wirbelauer, C., Schmidt, J., Burger, L., Krebs, A. R., Akalin, A., & Schübeler, D. (2015) · Nature · doi:10.1038/nature14176 · Cited by 705
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Sharma, S., De Carvalho, D. D., Jeong, S., Jones, P. A., & Liang, G. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1001286 · Cited by 119
Self-citationYou, J. S., Kelly, T. K., De Carvalho, D. D., Taberlay, P. C., Liang, G., & Jones, P. A. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1111309108 · Cited by 115
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Metadata
- Authors
- Jeong, S., Liang, G., Sharma, S., Lin, J. C., Choi, S. H., Han, H., Yoo, C. B., Egger, G., Yang, A. S., & Jones, P. A.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2009-10-01
- Volume
- 29
- Issue
- 19
- Pages
- 5366-5376
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2135173446
- Cited by
- 192
- References
- 6
- FWCI
- 8.52
- Type
- journal article
- Language
- en
- Open access
- green
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- Jeong, S., Liang, G., Sharma, S., Lin, J. C., Choi, S. H., Han, H., Yoo, C. B., Egger, G., Yang, A. S., & Jones, P. A. (2009). Selective Anchoring of DNA Methyltransferases 3A and 3B to Nucleosomes Containing Methylated DNA. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.00484-09
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