The DOI matches its Crossref record; 62 of the 64 references it cites were found in a registry or an index, and 2 are not checked yet.
Expression of cyr61, a growth factor-inducible immediate-early gene.
Open accessAbstract
A set of immediate-early genes that are rapidly activated by serum or purified platelet-derived growth factor in mouse 3T3 fibroblasts has been previously identified. Among these genes, several are related to known or putative transcription factors and growth factors, supporting the notion that some of these genes encode regulatory molecules important to cell growth. We show here that a member of this set of genes, cyr61 (originally identified by its cDNA 3CH61), encodes a 379-amino-acid polypeptide rich in cysteine residues. cyr61 can be induced through protein kinase C-dependent and -independent pathways. Unlike many immediate-early genes that are transiently expressed, the cyr61 mRNA is accumulated from the G0/G1 transition through mid-G1. This expression pattern is due to persistent transcription, while the mRNA is rapidly turned over during the G0/G1 transition and in mid-G1 at the same rate. In logarithmically growing cells, the cyr61 mRNA level is constant throughout the cell cycle. Cyr61 contains an N-terminal secretory signal sequence; however, it is not detected in the culture medium by immunoprecipitation. Cyr61 is synthesized maximally at 1 to 2 h after serum stimulation and has a short half-life within the cell.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Citations
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,460
Kozak, M. (1986) · Cell · doi:10.1016/0092-8674(86)90762-2 · Cited by 4,741
Shaw, G. D., & Kamen, R. (1986) · Cell · doi:10.1016/0092-8674(86)90341-7 · Cited by 3,946
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene
Greenberg, M. E., & Ziff, E. B. (1984) · Nature · doi:10.1038/311433a0 · Cited by 2,992
Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor
Kelly, K., Cochran, B., Stiles, C. D., & Leder, P. (1983) · Cell · doi:10.1016/0092-8674(83)90092-2 · Cited by 2,227
G 1 Events and Regulation of Cell Proliferation
Pardee, A. B. (1989) · Science · doi:10.1126/science.2683075 · Cited by 2,182
Early Signals in the Mitogenic Response
Rozengurt, E. (1986) · Science · doi:10.1126/science.3018928 · Cited by 1,444
Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
Montminy, M., Sevarino, K. A., Wagner, J. A., Mandel, G., & Goodman, R. H. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.18.6682 · Cited by 1,385
Sukhatme, V. P., Cao, X., Chang, L. C., Tsai‐Morris, C., Stamenkovich, D., Ferreira, P. C. P., Cohén, D., Edwards, S. A., Shows, T. B., Curran, T., et al. (1988) · Cell · doi:10.1016/0092-8674(88)90485-0 · Cited by 1,249
Flagged first, then by citations
Cited by
Sorted by citations
The Insulin-Like Growth Factor-Binding Protein (IGFBP) Superfamily
Hwa, V., Oh, Y., & Rosenfeld, R. G. (1999) · Endocrine Reviews · doi:10.1210/edrv.20.6.0382 · Cited by 1,076
Jun, J.-I., & Lau, L. F. (2010) · Nature Cell Biology · doi:10.1038/ncb2070 · Cited by 998
Self-citationConnective tissue growth factor: a mediator of TGF-β action on fibroblasts
Grotendorst, G. R. (1997) · Cytokine & Growth Factor Reviews · doi:10.1016/s1359-6101(97)00010-5 · Cited by 754
Frazier, K. S., Williams, S. J., Kothapalli, D., Klapper, H., & Grotendorst, G. R. (1996) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12363389 · Cited by 750
Igarashi, A., Okochi, H., Bradham, D. M., & Grotendorst, G. R. (1993) · Molecular Biology of the Cell · doi:10.1091/mbc.4.6.637 · Cited by 699
Metadata
- Authors
- O'Brien, T. P., Yang, G. P., Sanders, L., & Lau, L. F.
- Affiliations
- University of Illinois Chicago (US)
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1990-07
- Volume
- 10
- Issue
- 7
- Pages
- 3569-3577
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC360792
- OpenAlex
- W1793987231
- Cited by
- 310
- References
- 58
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 8 · 2017: 8 · 2016: 9 · 2015: 11 · 2014: 6 · 2013: 4 · 2012: 2
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- Funders
- American Cancer Society
- March of Dimes Foundation
- National Institutes of Health
- National Cancer Institute (R01CA046565, R01CA052220)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connective Tissue Growth Factor Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- CYR61, immediate early genes, platelet-derived growth factor, serum stimulation, 3T3 fibroblasts, protein kinase C, cell cycle regulation, mRNA turnover, secretory signal sequence, gene expression regulation, cell growth
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Cell Division, Cell Line, Cells, Cultured, Culture Media, DNA, Genes, Growth Substances, Humans, Molecular Sequence Data, Platelet-Derived Growth Factor, Proteins, RNA, Messenger, Transcription, Genetic, Protein Biosynthesis, Restriction Mapping, Gene Expression, Immediate-Early Proteins, Intercellular Signaling Peptides and Proteins, Mice, Cysteine-Rich Protein 61
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
