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Cited by 310Connective Tissue Growth Factor ResearchSignaling Pathways in DiseaseProtein Kinase Regulation and GTPase Signaling

The DOI matches its Crossref record; 62 of the 64 references it cites were found in a registry or an index, and 2 are not checked yet.

Expression of cyr61, a growth factor-inducible immediate-early gene.

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O'Brien, T P, Yang, G P, Sanders, L, Lau, L F·1990·Molecular and Cellular Biology
DOI 10.1128/mcb.10.7.3569PMC PMC360792
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Abstract

A set of immediate-early genes that are rapidly activated by serum or purified platelet-derived growth factor in mouse 3T3 fibroblasts has been previously identified. Among these genes, several are related to known or putative transcription factors and growth factors, supporting the notion that some of these genes encode regulatory molecules important to cell growth. We show here that a member of this set of genes, cyr61 (originally identified by its cDNA 3CH61), encodes a 379-amino-acid polypeptide rich in cysteine residues. cyr61 can be induced through protein kinase C-dependent and -independent pathways. Unlike many immediate-early genes that are transiently expressed, the cyr61 mRNA is accumulated from the G0/G1 transition through mid-G1. This expression pattern is due to persistent transcription, while the mRNA is rapidly turned over during the G0/G1 transition and in mid-G1 at the same rate. In logarithmically growing cells, the cyr61 mRNA level is constant throughout the cell cycle. Cyr61 contains an N-terminal secretory signal sequence; however, it is not detected in the culture medium by immunoprecipitation. Cyr61 is synthesized maximally at 1 to 2 h after serum stimulation and has a short half-life within the cell.

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Metadata

Authors
O'Brien, T. P., Yang, G. P., Sanders, L., & Lau, L. F.
Affiliations
University of Illinois Chicago (US)
ORCID
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1990-07
Volume
10
Issue
7
Pages
3569-3577
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.10.7.3569
PMC
PMC360792
OpenAlex
W1793987231
Cited by
310
References
58
Citations by year
2026: 2 · 2025: 5 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 8 · 2017: 8 · 2016: 9 · 2015: 11 · 2014: 6 · 2013: 4 · 2012: 2
FWCI
2.47
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
Funders
  • American Cancer Society
  • March of Dimes Foundation
  • National Institutes of Health
  • National Cancer Institute (R01CA046565, R01CA052220)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connective Tissue Growth Factor Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
CYR61, immediate early genes, platelet-derived growth factor, serum stimulation, 3T3 fibroblasts, protein kinase C, cell cycle regulation, mRNA turnover, secretory signal sequence, gene expression regulation, cell growth
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Cell Division, Cell Line, Cells, Cultured, Culture Media, DNA, Genes, Growth Substances, Humans, Molecular Sequence Data, Platelet-Derived Growth Factor, Proteins, RNA, Messenger, Transcription, Genetic, Protein Biosynthesis, Restriction Mapping, Gene Expression, Immediate-Early Proteins, Intercellular Signaling Peptides and Proteins, Mice, Cysteine-Rich Protein 61

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