Cited by 310Connective Tissue Growth Factor ResearchSignaling Pathways in DiseaseProtein Kinase Regulation and GTPase Signaling

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Expression of cyr61, a growth factor-inducible immediate-early gene.

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O'Brien, T P, Yang, G P, Sanders, L, Lau, L F·1990·Molecular and Cellular Biology

DOI 10.1128/mcb.10.7.3569PMC PMC360792

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Abstract

A set of immediate-early genes that are rapidly activated by serum or purified platelet-derived growth factor in mouse 3T3 fibroblasts has been previously identified. Among these genes, several are related to known or putative transcription factors and growth factors, supporting the notion that some of these genes encode regulatory molecules important to cell growth. We show here that a member of this set of genes, cyr61 (originally identified by its cDNA 3CH61), encodes a 379-amino-acid polypeptide rich in cysteine residues. cyr61 can be induced through protein kinase C-dependent and -independent pathways. Unlike many immediate-early genes that are transiently expressed, the cyr61 mRNA is accumulated from the G0/G1 transition through mid-G1. This expression pattern is due to persistent transcription, while the mRNA is rapidly turned over during the G0/G1 transition and in mid-G1 at the same rate. In logarithmically growing cells, the cyr61 mRNA level is constant throughout the cell cycle. Cyr61 contains an N-terminal secretory signal sequence; however, it is not detected in the culture medium by immunoprecipitation. Cyr61 is synthesized maximally at 1 to 2 h after serum stimulation and has a short half-life within the cell.

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Authors: O'Brien, T. P., Yang, G. P., Sanders, L., & Lau, L. F.
Affiliations: University of Illinois Chicago (US)
ORCID: Yang, G P
Lau, L F
Venue: Molecular and Cellular Biology
Publisher: Informa UK Limited
Published: 1990-07
Volume: 10
Issue: 7
Pages: 3569-3577
ISSN: 0270-7306 (ISSN-L)
1098-5549
DOI: 10.1128/mcb.10.7.3569
PMC: PMC360792
OpenAlex: W1793987231
Cited by: 310
References: 58
Citations by year: 2026: 2 · 2025: 5 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 8 · 2017: 8 · 2016: 9 · 2015: 11 · 2014: 6 · 2013: 4 · 2012: 2
FWCI: 2.47
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Molecular and Cellular Biology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
Funders: American Cancer Society
March of Dimes Foundation
National Institutes of Health
National Cancer Institute (R01CA046565, R01CA052220)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connective Tissue Growth Factor Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords: CYR61, immediate early genes, platelet-derived growth factor, serum stimulation, 3T3 fibroblasts, protein kinase C, cell cycle regulation, mRNA turnover, secretory signal sequence, gene expression regulation, cell growth
MeSH terms: Amino Acid Sequence, Animals, Base Sequence, Cell Division, Cell Line, Cells, Cultured, Culture Media, DNA, Genes, Growth Substances, Humans, Molecular Sequence Data, Platelet-Derived Growth Factor, Proteins, RNA, Messenger, Transcription, Genetic, Protein Biosynthesis, Restriction Mapping, Gene Expression, Immediate-Early Proteins, Intercellular Signaling Peptides and Proteins, Mice, Cysteine-Rich Protein 61

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Expression of cyr61, a growth factor-inducible immediate-early gene.

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