The DOI matches its Crossref record; 72 of the 73 references it cites were found in a registry or an index.
PU.1 (Spi-1) and C/EBPα Regulate Expression of the Granulocyte-Macrophage Colony-Stimulating Factor Receptor α Gene
Open accessAbstract
Growth factor receptors play an important role in hematopoiesis. In order to further understand the mechanisms directing the expression of these key regulators of hematopoiesis, we initiated a study investigating the transcription factors activating the expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor alpha gene. Here, we demonstrate that the human GM-CSF receptor alpha promoter directs reporter gene activity in a tissue-specific fashion in myelomonocytic cells, which correlates with its expression pattern as analyzed by reverse transcription PCR. The GM-CSF receptor alpha promoter contains an important functional site between positions -53 and -41 as identified by deletion analysis of reporter constructs. We show that the myeloid and B cell transcription factor PU.1 binds specifically to this site. Furthermore, we demonstrate that a CCAAT site located upstream of the PU.1 site between positions -70 and -54 is involved in positive-negative regulation of the GM-CSF receptor alpha promoter activity. C/EBP alpha is the major CCAAT/enhancer-binding protein (C/EBP) form binding to this site in nuclear extracts of U937 cells. Point mutations of either the PU.1 site or the C/EBP site that abolish the binding of the respective factors result in a significant decrease of GM-CSF receptor alpha promoter activity in myelomonocytic cells only. Furthermore, we demonstrate that in myeloid and B cell extracts, PU.1 forms a novel, specific, more slowly migrating complex (PU-SF) when binding the GM-CSF receptor alpha promoter PU.1 site. This is the first demonstration of a specific interaction with PU.1 on a myeloid PU.1 binding site. The novel complex is distinct from that described previously as binding to B cell enhancer sites and can be formed by addition of PU.1 to extracts from certain nonmyeloid cell types which do not express PU.1, including T cells and epithelial cells, but not from erythroid cells. Furthermore, we demonstrate that the PU-SF complex binds to PU.1 sites found on a number of myeloid promoters, and its formation requires an intact PU.1 site adjacent to a single-stranded region. Expression of PU.1 in nonmyeloid cells can activate the GM-CSF receptor alpha promoter. Deletion of the amino-terminal region of PU.1 results in a failure to form the PU-SF complex and in a concomitant loss of transactivation, suggesting that formation of the PU-SF complex is of functional importance for the activity of the GM-CSF receptor alpha promoter. Finally, we demonstrate that C/EBP alpha can also active the GM-CSF receptor alpha promoter in nonmyeloid cells. These results suggest that PU.1 and C/EBP alpha direct the cell-type-specific expression of GM-CSF receptor alpha, further establish the role of PU.1 as a key regulator of hematopoiesis, and point to C/EBP alpha as an additional important factor in this process.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“PU.1 imparts its key functions by regulating vast number of myeloid gene promoters that include GM-CSF receptor a, M-CSF receptor (M-CSFR), scavenger receptor CD163, and CD11b, etc (6,7).”
From FBW7 Inhibits Myeloid Differentiation in Acute Myeloid Leukemia via GSK3-Dependent Ubiquitination of PU.1 (2021) · cites this paper as [6]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In cotransfection experiments, RUNX1, PU.1, and CEBPA play essential roles in activation of the hematopoietic differentiation genes MCSFR and GMCSFR.7,8,31”
From RUNX1 regulates corepressor interactions of PU.1 (2011) · cites this paper as [31]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The block in the development of these lineages occurs at very early stages, because the mutant animals completely lack lymphoid and myeloid progenitor cells, rendering it difficult to determine the precise role of PU.1 during stage- and lineage-specific gene expression.”
From AKT Induces Transcriptional Activity of PU.1 through Phosphorylation-mediated Modifications within Its Transactivation Domain (2001) · cites this paper as [67]
Experimental — accuracy not yet measuredIn the citing paper
“In myeloid lineages, the putative PU.1 target genes include G-SCF receptor [23], GM-CSF receptor [24] and M-CSF receptor [25].”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [24]
In the citing paper
“C/EBP-α expression is prominent in immature myeloid cells [24, 101].”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [24]
In the citing paper
“Each factor has been shown to synergize on various promoters, including M-CSF receptor promoter and neutrophil elastase (NE) promoter [24, 63].”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [24]
In the citing paper
“C/EBP-␣ minimally activates several myeloid cell-specific promoters, including those for cytokine receptors (e.g., GM-CSF, G-CSF, and M-CSF receptors) (26,65,78,79) and granule proteins (e.g., NE) (50).”
From The t(8;21) Fusion Product, AML-1–ETO, Associates with C/EBP-α, Inhibits C/EBP-α-Dependent Transcription, and Blocks Granulocytic Differentiation (1998) · cites this paper as [26]
In the citing paper
“Moreover, C/EBP-␣ cooperates with other myeloid transcription factors, including AML-1B and PU.1, to synergistically upregulate the expression of several myeloid cellspecific promoters (26,50,78).”
From The t(8;21) Fusion Product, AML-1–ETO, Associates with C/EBP-α, Inhibits C/EBP-α-Dependent Transcription, and Blocks Granulocytic Differentiation (1998) · cites this paper as [26]
In the citing paper
“Each factor has been shown to synergize on various promoters, including M-CSF receptor promoter and neutrophil elastase promoter.15,35”
From Granulocyte inducer C/EBPα inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions (2002) · cites this paper as [15]
In the citing paper
“Targeted disruption of PU.1 in mice severely impairs the development of lymphoid (B cells) and myeloid (macrophages and neutrophils) lineages.”
From AKT Induces Transcriptional Activity of PU.1 through Phosphorylation-mediated Modifications within Its Transactivation Domain (2001) · cites this paper as [67]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Dignam, J. D., Lebovitz, R. M., & Roeder, R. G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1475 · Cited by 11,262
A new method for sequencing DNA.
Maxam, A. M., & Gilbert, W. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.2.560 · Cited by 7,929
Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.
Cao, Z., Umek, R. M., & McKnight, S. L. (1991) · Genes & Development · doi:10.1101/gad.5.9.1538 · Cited by 1,663
Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages
Scott, E. W., Simon, M. C., Anastasi, J., & Singh, H. (1994) · Science · doi:10.1126/science.8079170 · Cited by 1,532
EUKARYOTIC TRANSCRIPTIONAL REGULATORY PROTEINS
Johnson, P. F., & McKnight, S. L. (1989) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.58.070189.004055 · Cited by 1,210
Luciferase reporter gene vectors for analysis of promoters and enhancers.
Nordeen, S. K. (1988) · PubMed · Cited by 994
The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene
Klemsz, M. J., McKercher, S. R., Celada, A., Van Beveren, C., & Maki, R. A. (1990) · Cell · doi:10.1016/0092-8674(90)90219-5 · Cited by 954
Expression cloning of a receptor for human granulocyte‐macrophage colony‐stimulating factor.
Gearing, D. P., King, J. A., Gough, N. M., & Nicola, N. A. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb08541.x · Cited by 683
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes
Staudt, L. M., Singh, H., Sen, R., Wirth, T. C., Sharp, P. A., & Baltimore, D. (1986) · Nature · doi:10.1038/323640a0 · Cited by 658
Flagged first, then by citations
1 of the 73 references couldn’t be matched to a known paper.
Research works printed but not linked: 13
- Eisenbeis C. F., 1993, Mol. Cell. Biol., 13, 6452
- Emanuel P. D., 1991, Exp. Hematol., 19, 1017
- 10.1182/blood.V82.7.1960.1960 · DOI
- Moulton K. S., 1994, Mol. Cell. Biol., 14, 4408
- Nordeen S. K., 1988, BioTechniques, 6, 454
- 10.1128/mcb.14.8.5558-5568.1994 · DOI · Paper page
- Pahl H. L., 1991, Exp. Hematol., 19, 1038
- Perez C., 1994, Mol. Cell. Biol., 14, 5023
- Pongubala J. M., 1992, Mol. Cell. Biol., 12, 368
- Ray D., 1992, Mol. Cell. Biol., 12, 4297
- Schuetze S., 1992, Mol. Cell. Biol., 12, 2967
- Schuetze S., 1993, Mol. Cell. Biol., 13, 5670
- Zhang D. E., 1994, Mol. Cell. Biol., 14, 8085
Cited by
Sorted by citations
Items 101–200 of 286
Hikami, K., Kawasaki, A., Ito, I., Koga, M., Ito, S., Hayashi, T., Matsumoto, I., Tsutsumi, A., Kusaoi, M., Takasaki, Y., et al. (2011) · Arthritis & Rheumatism · doi:10.1002/art.30188 · Cited by 58
PU.1 controls the expression of long noncoding RNA HOTAIRM1 during granulocytic differentiation
Wei, S., Zhao, M., Wang, X., Li, Y., & Wang, K. (2016) · Journal of Hematology & Oncology · doi:10.1186/s13045-016-0274-1 · Cited by 58
“ceptor (GM-CSFR) [14–16], CD11b [17], CD18 [18], and myeloperoxidase [19].”
O’Reilly, D., Quinn, C. M., El‐Shanawany, T., Gordon, S., & Greaves, D. R. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212150200 · Cited by 56
Slámová, L., Starková, J., Froňková, E., Zaliova, M. K., Reznickova, L. R., van Delft, F. W., Vodickova, E., Volejníková, J., Zemanová, Z., Polgárová, K., et al. (2013) · Leukemia · doi:10.1038/leu.2013.354 · Cited by 57
The role of transcription factors in the guidance of granulopoiesis.
Fiedler, K., & Brunner, C. (2012) · PubMed Central · Cited by 56
Human Proteinase-3 Expression Is Regulated by PU.1 in Conjunction with a Cytidine-rich Element
Sturrock, A., Franklin, K. F., & Hoidal, J. R. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.50.32392 · Cited by 56
Lineage plasticity and commitment in T‐cell development
Rothenberg, E. V., & Dionne, C. J. (2002) · Immunological Reviews · doi:10.1034/j.1600-065x.2002.18709.x · Cited by 56
GATA2 regulates dendritic cell differentiation
Onodera, K., Fujiwara, T., Onishi, Y., Itoh‐Nakadai, A., Okitsu, Y., Fukuhara, N., Ishizawa, K., Shimizu, R., Yamamoto, M. P., & Harigae, H. (2016) · Blood · doi:10.1182/blood-2016-02-698118 · Cited by 53
Rieske, P., & Pongubala, J. R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m007482200 · Cited by 54
Gangenahalli, G. U., Gupta, P., Saluja, D., Verma, Y. K., Kishore, V., Chandra, R., Sharma, R. K., & Ravindranath, T. (2005) · Stem Cells and Development · doi:10.1089/scd.2005.14.140 · Cited by 53
“These promoters include the G-CSF (63), GM-CSF (64), and the M-CSF/c-fms (65) receptors; the high-affinity Fc (cid:4) I/CD64 (66) and the low-affinity Fc (cid:4) IIIA/CD16 receptors (67); the adhesion molecules CD11b and the CD18; proteinase-3, the scavenger receptor A gene and the tyrosine kinase c-fes/c-fos, whereas…”
InfluentialWang, W., Wang, X., Ward, A. C., Touw, I. P., & Friedman, A. (2001) · Leukemia · doi:10.1038/sj.leu.2402094 · Cited by 55
Transcription factor networks in dendritic cell development
Satpathy, A. T., MURPHY, K. M. M., & Wumesh, K. C. (2011) · Seminars in Immunology · doi:10.1016/j.smim.2011.08.009 · Cited by 57
“1 controls the early expression of Flt3, GM-CSFR, and M-CSFR on progenitors, although only the absence of Flt3 was confirmed on DC precursors [78;79].”
BackgroundMcIvor, Z., Hein, S., Fiegler, H., Schroeder, T., Stocking, C., Just, U., & Cross, M. (2003) · Experimental Hematology · doi:10.1016/s0301-472x(02)01017-2 · Cited by 58
Runx1/AML1 in leukemia: disrupted association with diverse protein partners
Perry, C., Eldor, A., & Soreq, H. (2002) · Leukemia Research · doi:10.1016/s0145-2126(01)00128-x · Cited by 51
Lutz, P. G., Houzel-Charavel, A., Moog‐Lutz, C., & Cayre, Y. E. (2001) · Blood · doi:10.1182/blood.v97.8.2449 · Cited by 50
Epigenetic control of hematopoiesis: the PU.1 chromatin connection
van Riel, B., & Rosenbauer, F. (2014) · Biological Chemistry · doi:10.1515/hsz-2014-0195 · Cited by 48
Transcriptional mechanisms regulating myeloid-specific genes
Skalnik, D. G. (2002) · Gene · doi:10.1016/s0378-1119(02)00387-6 · Cited by 48
Fukuoka, T., Kitami, Y., Okura, T., & Hiwada, K. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.36.25576 · Cited by 48
The ETS Family of Transcriptional Regulators
Ghysdael, J., & Boureux, A. (1997) · Birkhäuser Basel eBooks · doi:10.1007/978-3-0348-8889-9_2 · Cited by 48
Decreased Neutrophil Apoptosis in Quiescent ANCA-Associated Systemic Vasculitis
Abdgawad, M., Pettersson, Å., Gunnarsson, L., Bengtsson, A. A., Geborek, P., Nilsson, L., Segelmark, M., & Hellmark, T. (2012) · PLoS ONE · doi:10.1371/journal.pone.0032439 · Cited by 47
Role of Transcription Factors in Differentiation and Reprogramming of Hematopoietic Cells
Nakajima, H. (2011) · The Keio Journal of Medicine · doi:10.2302/kjm.60.47 · Cited by 50
Rao, S., Matsumura, A., Yoon, J. W., & Simon, M. C. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.16.11115 · Cited by 46
PU.1-c-Jun interaction is crucial for PU.1 function in myeloid development
Zhao, X., Bartholdy, B., Yamamoto, Y., Evans, E. K., Alberich-Jordà, M., Staber, P. B., Benoukraf, T., Zhang, P., Zhang, J., Trinh, B. Q., et al. (2022) · Communications Biology · doi:10.1038/s42003-022-03888-7 · Cited by 46
Transcriptional Regulation of Mouse μ Opioid Receptor Gene by PU.1
Hwang, C. K., Kim, C. S., Choi, H. S., McKercher, S. R., & Loh, H. H. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m400755200 · Cited by 46
Mutant CEBPA directly drives the expression of the targetable tumor-promoting factor CD73 in AML
Jakobsen, J. S., Laursen, L. G., Schuster, M. B., Pundhir, S., Schoof, E. M., Ge, Y. C., D’Altri, T., Vitting‐Seerup, K., Rapin, N., Gentil, C., et al. (2019) · Science Advances · doi:10.1126/sciadv.aaw4304 · Cited by 49
Myeloid ELF1-like Factor Is a Potent Activator of Interleukin-8 Expression in Hematopoietic Cells
Hedvat, C. V., Yao, J., Sokolic, R. A., & Nimer, S. D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m307524200 · Cited by 45
Transcriptional Regulation of Myelopoiesis
Friedman, A. D. (2002) · International Journal of Hematology · doi:10.1007/bf02982108 · Cited by 44
van Dijk, T. B., Caldenhoven, E., Raaijmakers, J. A. M., Lammers, J.-W. J., Koenderman, L., & de Groot, R. P. (1998) · Blood · doi:10.1182/blood.v91.6.2126 · Cited by 43
Tsiftsoglou, A. S., Bonovolias, I. D., & Tsiftsoglou, S. A. (2009) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2009.03.001 · Cited by 43
“…the expression of several factors modulating the expression of receptors, cytokines and enzymes that regulate the developmental program of hematopoiesis (D. E. Zhang et al., 1994; Nuchprayoon et al., 1994; Frank et al., 1995; Hohaus et al., 1995; D. E. Zhang et al., 1996; Uchida et al., 1997).”
BackgroundOkumura, A. J., Peterson, L. F., Okumura, F., Boyapati, A., & Zhang, D. (2008) · Blood · doi:10.1182/blood-2007-11-124735 · Cited by 42
Transcriptional Repression of the Neurofibromatosis-1 Tumor Suppressor by the t(8;21) Fusion Protein
Yang, G., Khalaf, W. F., van de Locht, L. T., Jansen, J. H., Gao, M., Thompson, M. A., van der Reijden, B. A., Gutmann, D. H., Delwel, R., Clapp, D. W., et al. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.14.5869-5879.2005 · Cited by 40
Cytomegalovirus IE2 Protein Stimulates Interleukin 1β Gene Transcription via Tethering to Spi-1/PU.1
Wara-aswapati, N., Yang, Z., Waterman, W. R., Koyama, Y., Tetradis, S., Choy, B. K., Webb, A. C., & Auron, P. E. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.10.6803 · Cited by 41
“Many of these involve various members of the ETS transcription factor family, including Spi-1 (1, 23, 30, 51, 58).”
BackgroundPark, S. D., Saunders, A. S., Reidy, M. A., Bender, D. E., Clifton, S. C., & Morris, K. T. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2022.932608 · Cited by 44
Transcriptional regulation of hemopoiesis
Barreda, D. R., & Belosevic, M. (2001) · Developmental & Comparative Immunology · doi:10.1016/s0145-305x(01)00035-0 · Cited by 40
Ito, T., Nishiyama, C., Nishiyama, M., Matsuda, H., Maeda, K., Akizawa, Y., Tsuboi, R., Okumura, K., & Ogawa, H. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.1.376 · Cited by 39
Kumar, A., Rani, L., Mhaske, S. T., Pote, S. T., Behera, S., Mishra, G. C., & Wani, M. R. (2020) · The Journal of Immunology · doi:10.4049/jimmunol.1801629 · Cited by 40
Mechanisms of myeloid leukemogenesis: Current perspectives and therapeutic objectives
Bouligny, I. M., Maher, K. R., & Grant, S. (2022) · Blood Reviews · doi:10.1016/j.blre.2022.100996 · Cited by 43
“These mutations disrupt the activation of transcription of the granulocyte colony-stimulating factor (G-CSF) receptor and the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor, resulting in the differentiation block [119,120].”
BackgroundChoe, K. S., Ujhelly, O., Wontakal, S. N., & Skoultchi, A. I. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.077727 · Cited by 38
Hauses, M., Tönjes, R. R., & Grez, M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.48.31844 · Cited by 35
Yamada, T., Abe, M., Higashi, T., Yamamoto, H., Kihara‐Negishi, F., Sakurai, T., Shirai, T., & Oikawa, T. (2001) · Blood · doi:10.1182/blood.v97.8.2300 · Cited by 38
Rosenbauer, F., Wagner, K., Zhang, P., Knobeloch, K., Iwama, A., & Tenen, D. G. (2004) · Blood · doi:10.1182/blood-2003-08-2688 · Cited by 35
Greulich, F., Hemmer, M. C., Rollins, D. A., Rogatsky, I., & Uhlenhaut, N. H. (2016) · Steroids · doi:10.1016/j.steroids.2016.05.003 · Cited by 35
“Conversely, loss of c/EBPα increases the proliferation and self-renewing capabilities of hematopoietic stem cells, and blocks the M-CSF-dependent differentiation towards the myeloid lineage [39, 40].”
BackgroundCroker, B. A., Mielke, L. A., Wormald, S., Metcalf, D., Kiu, H., Alexander, W. S., Hilton, D. J., & Roberts, A. W. (2008) · Experimental Hematology · doi:10.1016/j.exphem.2008.02.008 · Cited by 34
Lee, E. S., Sarma, D., Zhou, H., & Henderson, A. J. (2002) · Virology · doi:10.1006/viro.2002.1500 · Cited by 34
“C/EBP has also been demonstrated to interact with components of the basal transcriptional machinery such as TBP and TFIIB (Nerlov and Ziff, 1995) and other transcription factors, including NF- B and CREB (Hohaus et al., 1995; LeClair et al., 1992; Ross et al., 2001; Stein et al., 1993; Westendorf et al., 1998; Zhang e…”
BackgroundCombinatorial Determinants of Tissue-Specific Transcription in B Cells and Macrophages
Nikolajczyk, B. S., Cortes, M., Feinman, R., & Sen, R. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.7.3527 · Cited by 34
Ma, Y., Su, Q., & Tempst, P. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.15.8727 · Cited by 34
Androgen signaling is a confounding factor for β-catenin-mediated prostate tumorigenesis
Lee, S. H., Luong, R. H., Johnson, D. T., Cunha, G. R., Rivina, L., Gonzalgo, M. L., & Sun, Z. (2015) · Oncogene · doi:10.1038/onc.2015.117 · Cited by 33
Frecha, C. A., Toscano, M. G., Costa, C., Sáez‐Lara, M. J., Cosset, F., Verhoeyen, E., & Martı́n, F. (2008) · Gene Therapy · doi:10.1038/gt.2008.20 · Cited by 32
ELA2 is regulated by hematopoietic transcription factors, but not repressed by AML1-ETO
Lausen, J., Liu, S., Fliegauf, M., Lübbert, M., & Werner, M. H. (2005) · Oncogene · doi:10.1038/sj.onc.1209181 · Cited by 32
“Transcriptional synergy between AML1 and C/EBPa is well known (Hohaus et al., 1995; Oelgeschlager et al., 1996; Zhang et al., 1996; Westendorf et al., 1998).”
BackgroundKitami, Y., Fukuoka, T., Hiwada, K., & Inagami, T. (1999) · Circulation Research · doi:10.1161/01.res.84.1.64 · Cited by 33
Deregulated transcription factors in leukemia
Shima, Y., & Kitabayashi, I. (2011) · International Journal of Hematology · doi:10.1007/s12185-011-0905-9 · Cited by 33
Transcriptional Regulation in Neutrophils: Teaching Old Cells New Tricks
McDonald, P. P. (2004) · Advances in immunology · doi:10.1016/s0065-2776(04)82001-7 · Cited by 32
van Dijk, T. B., Baltus, B., Raaijmakers, J. A. M., Lammers, J. J., Koenderman, L., & de Groot, R. P. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.5.2674 · Cited by 35
Friedman, A. D. (2002) · Journal of Cellular Biochemistry · doi:10.1002/jcb.10271 · Cited by 32
“C/EBPa expression is highest in immature myeloid cells, whereas C/EBPb, C/EBPb, and C/EBPe expression increases during granulocyte or monocyte maturation [Scott et al., 1992; Hohaus et al., 1995].”
BackgroundGombart, A. F., Grewal, J., & Koeffler, H. P. (2007) · Journal of Leukocyte Biology · doi:10.1189/jlb.0806516 · Cited by 30
Identification of three genes up-regulated in PU.1 rescued monocytic precursor cells
Henkel, G. W. (2002) · International Immunology · doi:10.1093/intimm/dxf040 · Cited by 31
Mishra, M., Thacker, G., Sharma, A., Singh, A. K., Upadhyay, V., Sanyal, S., Verma, S. P., Tripathi, A. K., Bhatt, M. L. B., & Trivedi, A. K. (2021) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-20-0268 · Cited by 30
“1 imparts its key functions by regulating vast number of myeloid gene promoters that include GM-CSF receptor a, M-CSF receptor (M-CSFR), scavenger receptor CD163, and CD11b, etc (6, 7).”
BackgroundLentiviral PU.1 overexpression restores differentiation in myeloid leukemic blasts
Durual, S., Rideau, A., Ruault-Jungblut, S., Cossali, D., Beris, P., Piguet, V, & Matthes, T. (2007) · Leukemia · doi:10.1038/sj.leu.2404645 · Cited by 30
Integration of cytokine and transcription factor signals in hematopoietic stem cell commitment
Sarrazin, S., & Sieweke, M. H. (2011) · Seminars in Immunology · doi:10.1016/j.smim.2011.08.011 · Cited by 34
van Dijk, T. B., Baltus, B., Raaijmakers, J. A. M., Lammers, J. J., Koenderman, L., & de Groot, R. P. (1999) · PubMed · Cited by 29
FIP1L1-PDGFRα Imposes Eosinophil Lineage Commitment on Hematopoietic Stem/Progenitor Cells
Fukushima, K., Matsumura, I., Ezoe, S., Tokunaga, M., Yasumi, M., Satoh, Y., Shibayama, H., Tanaka, H., Iwama, A., & Kanakura, Y. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m807489200 · Cited by 27
Serum Amyloid A Proteins and Their Impact on Metastasis and Immune Biology in Cancer
Lee, J., & Beatty, G. L. (2021) · Cancers · doi:10.3390/cancers13133179 · Cited by 28
CCAAT-enhancer binding proteinα is expressed in activated microglial cells after brain injury
Walton, M., Saura, J., Young, D., MacGibbon, G., Hansen, W., Lawlor, P., Sirimanne, E., Gluckman, P., & Dragunow, M. (1998) · Molecular Brain Research · doi:10.1016/s0169-328x(98)00169-7 · Cited by 28
Mikhail, F. M., Coignet, L., Hatem, N., Mourad, Z., Farawela, H. M., Elkaffash, D. M., Farahat, N. M. G., & Nucifora, G. (2003) · Genes Chromosomes and Cancer · doi:10.1002/gcc.10302 · Cited by 28
“GM-CSF, M-CSF receptor, myeloperoxidase, neutrophil elastase, granzyme B, and subunits of the T-cell antigen receptor (Redondo et al., 1992; Suzow et al., 1993; Wang et al., 1993; Nuchprayoon et al., 1994; Zhang et al., 1994; Hohaus et al., 1995; Wargnier et al., 1995; Uchida et al., 1997).”
BackgroundThe transcription factor PU.1 does not regulate lineage commitment but has lineage-specific effects
McKercher, S. R., Henkel, G. W., & Maki, R. A. (1999) · Journal of Leukocyte Biology · doi:10.1002/jlb.66.5.727 · Cited by 28
Sonn, I., Honda‐Ozaki, F., Yoshimatsu, S., Morimoto, S., Watanabe, H., & Okano, H. (2022) · Inflammation and Regeneration · doi:10.1186/s41232-022-00201-1 · Cited by 30
“1 is reportedly crucial for the differentiation of both cell types [21, 62].”
BackgroundKatzenback, B. A., Katakura, F., & Belosevic, M. (2015) · Developmental & Comparative Immunology · doi:10.1016/j.dci.2015.10.024 · Cited by 28
Palani, S., & Sarkar, C. A. (2009) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000518 · Cited by 28
Hashimoto, S., Nishizumi, H., Hayashi, R., Tsuboi, A., Nagawa, F., Takemori, T., & Sakano, H. (1999) · International Immunology · doi:10.1093/intimm/11.9.1423 · Cited by 27
Hirouchi, T., Takabatake, T., Yoshida, K., Nitta, Y., Nakamura, M. M., Tanaka, S., Ichinohe, K., Oghiso, Y., & Tanaka, K. J. (2008) · Experimental Hematology · doi:10.1016/j.exphem.2008.01.015 · Cited by 27
Li, X., Vradii, D., Gutiérrez, S., Lian, J. B., van Wijnen, A. J., Stein, J. L., Stein, G. S., & Javed, A. (2005) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20548 · Cited by 27
“The human M-CSF receptor c-FMS promoter fragment ( 416 to þ71 bp) fused to the luciferase gene was a kind gift of D.G. Tenen, Harvard Institutes of Medicine [Hohaus et al., 1995].”
BackgroundMethodInfluentialvan Dijk, T. B., Baltus, B., Caldenhoven, E., Handa, H., Raaijmakers, J. A. M., Lammers, J. J., Koenderman, L., & de Groot, R. P. (1998) · Blood · doi:10.1182/blood.v92.10.3636 · Cited by 24
Humbert, M., Federzoni, E. A., Britschgi, A., Schläfli, A. M., Valk, P. J. M., Kaufmann, T., Haferlach, T., Behre, G., Simon, H., Torbett, B. E., et al. (2013) · Journal of Leukocyte Biology · doi:10.1189/jlb.1112608 · Cited by 26
Modeling the Hematopoietic Landscape
Brown, G., & Ceredig, R. (2019) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2019.00104 · Cited by 27
Molecular and Cellular Aspects of Macrophage Aging
Sebastián, C., Lloberas, J., & Celada, A. (2009) · Springer eBooks · doi:10.1007/978-1-4020-9063-9_46 · Cited by 24
Dominant-interfering C/EBPα stimulates primitive erythropoiesis in zebrafish
Liu, T. X., Rhodes, J., Deng, M., Hsu, K., Radomska, H. S., Kanki, J. P., Tenen, D. G., & Look, A. T. (2007) · Experimental Hematology · doi:10.1016/j.exphem.2006.10.008 · Cited by 24
“CEBPA is strongly expressed in granulocyte/monocyte progenitors (GMPs) of human and rodent bone marrow and is specifically upregulated during granulocytic differentiation [1-4].”
BackgroundKoyama, N., Hoelzer, D. F., & Ottmann, O. G. (2004) · European Journal of Immunology · doi:10.1002/eji.200324420 · Cited by 22
The Granulocyte Colony-Stimulating Factor Receptor and Its Role in Disorders of Granulopoiesis
Avalos, B. R. (1998) · Leukemia & lymphoma/Leukemia and lymphoma · doi:10.3109/10428199809092682 · Cited by 22
Taylor, S. J., Stauber, J., Bohorquez, O., Tatsumi, G., Kumari, R., Chakraborty, J., Bartholdy, B. A., Schwenger, E., Sundaravel, S., Farahat, A. A., et al. (2024) · Nature Genetics · doi:10.1038/s41588-024-01911-7 · Cited by 22
Uchiumi, F., Sakakibara, G., Sato, J., & Tanuma, S.-I. (2008) · Genes to Cells · doi:10.1111/j.1365-2443.2008.01240.x · Cited by 21
“PU.1-binding sequences are found in promoter regions of proteins, including macrophage colony-stimulating factor (M-CSF) receptor (Zhang et al. 1994), and granulocyte-macrophage colonystimulating factor (GM-CSF) receptor α (Hohaus et al. 1995).”
BackgroundHematopoietic Stem Cell Molecular Targets and Factors Essential for Hematopoiesis
Raghav, P. K., & Gangenahalli, G. U. (2018) · Journal of Stem Cell Research & Therapy · doi:10.4172/2157-7633.1000441 · Cited by 22
“1 also interacts synergistically or antagonistically with c-Jun, C/EBPδ, GATA-1, GATA-2, C/EBPα, c-Myb, CBP, AML-1B, AML-1/ETO, NF-EMS/Pip/IRF-4, and ICSBP/IRF-8, which governs most of the genes [37-47].”
BackgroundTranscriptional and epigenetic regulation of the GM-CSF promoter by RUNX1
Taberlay, P. C., James, S. R., Qadi, A. A., West, A. C., Ray, S., Bert, A. G., Cockerill, P. N., & Holloway, A. F. (2010) · Leukemia Research · doi:10.1016/j.leukres.2010.03.029 · Cited by 22
AML1B transcriptional repressor function is impaired by the Flt3‐internal tandem duplication
Takahashi, S., Harigae, H., Kameoka, J., Sasaki, T., & Kaku, M. (2005) · British Journal of Haematology · doi:10.1111/j.1365-2141.2005.05621.x · Cited by 21
“These include the genes encoding IL-3 (Uchida et al, 1997), granulocyte–macrophage colony-stimulating factor (GM-CSF) (Hohaus et al, 1995), CSF 1 receptor (Zhang et al, 1994a), protein kinase C beta (Hug et al, 2004), and p21 (Lutterbach et al, 2000).”
BackgroundTerminal Differentiation of Dendritic Cells
Seillet, C., & Belz, G. T. (2013) · Advances in immunology · doi:10.1016/b978-0-12-417028-5.00007-7 · Cited by 25
Popernack, P. M., Truong, L., Kamphuis, M. M. J., & Henderson, A. J. (2001) · Stem Cells and Development · doi:10.1089/152581601753193841 · Cited by 19
A minimal c-fes cassette directs myeloid-specific expression in transgenic mice
Heydemann, A., Warming, S., Clendenin, C., Sigrist, K. S., Hjorth, J. P., & Simon, M. C. (2000) · Blood · doi:10.1182/blood.v96.9.3040 · Cited by 20
Faust, N., Bonifer, C., & Sippel, A. E. (1999) · DNA and Cell Biology · doi:10.1089/104454999315042 · Cited by 20
“…example, been found in the promoter regions of the genes for macrophage colony-stimulating factor receptor (M-CSFR) or granulocyte colony-stimulating factor receptor (G-CSFR) (Zhang et al. , 1996a; Hohaus et al. , 1995) as well as the 2 6.1 kb chicken lysozyme enhancer (Goethe and Phi Van, 1994).”
InfluentialRooney, J. W., & Calame, K. L. (2001) · Genes & Development · doi:10.1101/gad.937201 · Cited by 20
RetractedC/EBPβ regulates human immunodeficiency virus 1 gene expression through its association with cdk9
Mameli, G., Deshmane, S. L., Ghafouri, M., Cui, J., Simbiri, K. O., Khalili, K., Mukerjee, R., Dolei, A., Amini, S., & Sawaya, B. E. (2007) · Journal of General Virology · doi:10.1099/vir.0.82487-0 · Cited by 19
“…to interact with components of the basal transcriptional machinery such as the TATA-binding protein and transcription factor IIB (Nerlov & Ziff, 1995) and other transcription factors, including Nf-kB and the cAMP response element-binding protein (Hohaus et al., 1995; Westendorf et al., 1998).”
BackgroundMiliti, S., Riccioni, R., Parolini, I., Sposi, N. M., Samoggia, P., Pelosi, E., Testa, U., & Peschle, C. (2000) · British Journal of Haematology · doi:10.1046/j.1365-2141.2000.02348.x · Cited by 19
PU.1 regulates glutathione peroxidase expression in neutrophils
Throm, S. L., & Klemsz, M. J. (2003) · Journal of Leukocyte Biology · doi:10.1189/jlb.0203061 · Cited by 19
Sun, Z., Yergeau, D. A., Wong, I. C., Tuypens, T., Tavernier, J. H., Paul, C. C., Baumann, M. A., Auron, P. E., Tenen, D. G., & Ackerman, S. J. (1996) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-85232-9_18 · Cited by 19
RUNX1-RUNX1 Homodimerization Modulates RUNX1 Activity and Function
Li, D., Sinha, K. K., Abdul‐Hay, M., Rinaldi, C. R., Saunthararajah, Y., & Nucifora, G. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m700074200 · Cited by 18
Dendritic Cell Development: A Choose-Your-Own-Adventure Story
Moore, A. J., & Anderson, M. K. (2013) · Advances in Hematology · doi:10.1155/2013/949513 · Cited by 18
Transcriptional Regulation of c-fms Gene Expression
Xie, Y., Chen, C., & Hume, D. A. (2001) · Cell Biochemistry and Biophysics · doi:10.1385/cbb:34:1:001 · Cited by 19
Hong, S., Lee, M. Y., & Cheong, J. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4727 · Cited by 18
PU.1 regulates the expression of the human neutrophil elastase gene
Srikanth, S., & Rado, T. A. (1998) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(98)00039-6 · Cited by 18
Exploring the Interplay between Bone Marrow Stem Cells and Obesity
Moscatelli, F., Monda, A., Messina, G., Picciocchi, E., Monda, M., di Padova, M., Monda, V., Mezzogiorno, A., Dipace, A., Limone, P., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25052715 · Cited by 17
Sive, J. I., Basilico, S., Hannah, R., Kinston, S., Calero‐Nieto, F. J., & Göttgens, B. (2015) · Leukemia · doi:10.1038/leu.2015.172 · Cited by 17
Diabetes Inhibits Gr-1+ Myeloid Cell Maturation via Cebpa Deregulation
Wicks, K., Torbica, T., Umehara, T., Amin, S., Bobola, N., & Mace, K. A. (2015) · Diabetes · doi:10.2337/db14-1895 · Cited by 17
“In addition, CEBPA activates CSf2ra, which encodes GM-CSF receptor a (GM-CSFRa) (31) and is downregulated by 0.”
Background
Metadata
- Authors
- Hohaus, S., Petrovick, M. S., Voso, M. T., Sun, Z., Zhang, D.-E., & Tenen, D. G.
- Affiliations
- Harvard University (US)
- Corresponding author
- Tenen, Daniel G.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1995-10-01
- Volume
- 15
- Issue
- 10
- Pages
- 5830-5845
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC230835
- OpenAlex
- W2161743101
- Cited by
- 284
- References
- 73
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 4 · 2023: 5 · 2022: 8 · 2021: 3 · 2020: 6 · 2019: 6 · 2018: 7 · 2017: 5 · 2016: 5 · 2015: 8 · 2014: 5 · 2013: 7 · 2012: 6
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Cineca Institutional Research Information System (Tor Vergata University) (submittedVersion)
- Funders
- American Lung Association
- National Cancer Institute (CA 41456, R01CA041456)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK048660, DK48660)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- PU.1, SPI-1, C/EBPα, GM-CSF receptor alpha, transcription factor binding sites, myelomonocytic cells, B cells, promoter regulation, transcriptional activation, hematopoiesis, single-stranded DNA binding, U937 cells
- MeSH terms
- Amino Acid Sequence, B-Lymphocytes, Base Sequence, Cell Line, DNA, DNA-Binding Proteins, DNA, Single-Stranded, Hematopoiesis, Humans, Methylation, Molecular Sequence Data, Monocytes, Mutation, Nuclear Proteins, Promoter Regions, Genetic, T-Lymphocytes, Transcription Factors, Transcriptional Activation, Retroviridae Proteins, Oncogenic, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor, Genes, Reporter, CCAAT-Enhancer-Binding Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
