Stat3 Activation Is Required for Cellular Transformation by v- src
Open accessAbstract
Stat3 activation has been associated with cytokine-induced proliferation, anti-apoptosis, and transformation. Constitutively activated Stat3 has been found in many human tumors as well as v-abl- and v-src-transformed cell lines. Because of these correlations, we examined directly the relationship of activated Stat3 to cellular transformation and found that wild-type Stat3 enhances the transforming potential of v-src while three dominant negative Stat3 mutants inhibit v-src transformation. Stat3 wild-type or mutant proteins did not affect v-ras transformation. We conclude that Stat3 has a necessary role in v-src transformation.
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Activation of STAT3 signaling is a critical component of malignant transformation.
“Further study revealed that STAT3 activation and specific gene regulation is required for Src-mediated transformation of NIH-3T3 cells, leading to the conclusion that activation of STAT3 signaling is a critical component of malignant transformation.42,43”
From Critical analysis of the potential for targeting STAT3 in human malignancy (2013) · cites this paper as [42]
The Discussion (page not available in our copy) reports that activated Stat3 has oncogenic potential and that inhibiting Stat3 reduces v-src transformation in NIH 3T3 cells. The paper concludes that Stat3 activation is necessary for v-src transformation, but does not establish that it is required for malignant transformation generally; it reports no effect on v-ras transformation. (LLM, conf=0.88)
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Cites
Liver Failure and Defective Hepatocyte Regeneration in Interleukin-6-Deficient Mice
Cressman, D. E., Greenbaum, L. E., DeAngelis, R. A., Ciliberto, G., Furth, E. E., Poli, V., & Taub, R. (1996) · Science · doi:10.1126/science.274.5291.1379 · Cited by 1,536 · see citing works
Activation and Association of Stat3 with Src in v-Src-Transformed Cell Lines
Cao, X., Tay, A., Guy, G. R., & Tan, Y. H. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.4.1595 · Cited by 359 · see citing works
Flagged first, then by citations
Research works printed but not linked: 6
- [1]1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1994. Current protocols in molecular biology. John Wiley and Sons, New York, N.Y.
- [4]4. Bromberg, J. F., C. M. Horvath, Z. Wen, R. D. Schreiber, and J. E. Darnell, Jr. 1996. Transcriptionally active Stat1 is required for the antiproliferative effects of both interferon alpha and interferon gamma. Proc. Natl. Acad. Sci. USA 93: 7673-7678.
- [22]22. Minami, M., M. Inoue, S. Wei, K. Takeda, M. Matsumoto, T. Kishimoto, and S. Akira. 1996. Stat3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA 93: 3963-3966.
- [30]30. Takeda, K., K. Noguchi, W. Shi, T. Tanaka, M. Matsumoto, N. Yoshida, T. Kishimoto, and S. Akira. 1997. Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality. Proc. Natl. Acad. Sci. USA 94: 3801-3804.
- [29]29. Suematsu, S., T. Matsusaka, T. Matsuda, S. Ohno, J. Miyazaki, K. Yamamura, T. Hirano, and T. Kishimoto. 1992. Generation of plasmacytomas with the chromosomal translocation t(12;15) in interleukin 6 transgenic mice. Proc. Natl. Acad. Sci. USA 89: 232-235.
- [36]36. Zhang, Q., I. Nowak, E. C. Vonderheid, A. H. Rook, M. E. Kadin, P. C. Nowell, L. M. Shaw, and M. A. Wasik. 1996. Activation of Jak/STAT proteins involved in signal transduction pathway mediated by receptor for interleukin 2 in malignant T lymphocytes derived from cutaneous anaplastic large T-cell lymphoma and Sezary syndrome. Proc. Natl. Acad. Sci. USA 93: 9148-9153.
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Sorted by citations
Bowman, T., Garcia, R., Turkson, J., & Jove, R. (2000) · Oncogene · doi:10.1038/sj.onc.1203527 · Cited by 1,743 · see citing works
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Bromberg, J., & Darnell, J. E. (2000) · Oncogene · doi:10.1038/sj.onc.1203476 · Cited by 1,201 · see citing works
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Pathria, P., Louis, T. L., & Varner, J. A. (2019) · Trends in Immunology · doi:10.1016/j.it.2019.02.003 · Cited by 933 · see citing works
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Pollen, A. A., Nowakowski, T. J., Chen, J., Retallack, H., Sandoval-Espinosa, C., Nicholas, C. R., Shuga, J., Liu, S. J., Oldham, M. C., Diaz, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.004 · Cited by 908 · see citing works
Epling-Burnette, P. K., Liu, J. H., Catlett-Falcone, R., Turkson, J., Oshiro, M., Kothapalli, R., Li, Y., Wang, J.-M., Yang-Yen, H.-F., Karras, J., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci9940 · Cited by 633 · see citing works
Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis
Bowman, T., Broome, M. A., Sinibaldi, D., Wharton, W., Pledger, W. J., Sedivy, J. M., Irby, R., Yeatman, T., Courtneidge, S. A., & Jove, R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.131568898 · Cited by 488 · see citing works
Activation of STAT3 by the Hepatitis C Virus Core Protein Leads to Cellular Transformation
Yoshida, T., Hanada, T., Tokuhisa, T., Kosai, K.-I., Sata, M., Kohara, M., & Yoshimura, A. (2002) · The Journal of Experimental Medicine · doi:10.1084/jem.20012127 · Cited by 236 · see citing works
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Geiger, J. L., Grandis, J. R., & Bauman, J. E. (2016) · Oral Oncology · doi:10.1016/j.oraloncology.2015.11.022 · Cited by 193 · see citing works
Campbell, C. L., Jiang, Z., Savarese, D. M. F., & Savarese, T. M. (2001) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)63940-5 · Cited by 151 · see citing works
Dasatinib sensitizes KRAS mutant colorectal tumors to cetuximab
Dunn, E. F., Iida, M., Myers, R. A., Campbell, D. A., Hintz, K. A., Armstrong, E. A., Li, C., & Wheeler, D. L. (2011) · Oncogene · doi:10.1038/onc.2010.430 · Cited by 133 · see citing works
“…targets including the EGFR, STATS, PLCγ, PKCs, FAK, RAS, RAF and mucin 1 (MUC1) (Biscardi et al., 2000; Bivona et al., 2003; Blake et al., 1999; Bromberg et al., 1998; Chiu et al., 2002; Denning et al., 1996; Fabian et al., 1993; Joseloff et al., 2002; Kijima et al., 2002; Kronfeld et al.,…”
BackgroundCourapied, S., Sellier, H., de Carné Trécesson, S., Vigneron, A., Bernard, A.-C., Gamelin, E., Barré, B., & Coqueret, O. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.092304 · Cited by 76 · see citing works
Griffiths, G. J., Koh, M. Y., Brunton, V. G., Cawthorne, C., Reeves, N. A., Greaves, M., Tilby, M. J., Pearson, D. G., Ottley, C. J., Workman, P., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m408550200 · Cited by 60 · see citing works
An androgen-IL-6-Stat3 autocrine loop re-routes EGF signal in prostate cancer cells.
Aaronson, D. S., Muller, M., Neves, S. R., Chung, W.-C., Jayaram, G., Iyengar, R., & Ram, P. T. (2007) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2007.02.006 · Cited by 47 · see citing works
Critical analysis of the potential for targeting STAT3 in human malignancy
Grandis, J., & Peyser (2013) · OncoTargets and Therapy · doi:10.2147/ott.s47903 · Cited by 24 · see citing works
“Further study revealed that STAT3 activation and specific gene regulation is required for Src-mediated transformation of NIH-3T3 cells, leading to the conclusion that activation of STAT3 signaling is a critical component of malignant transformation.(42,43) Additional studies generated similar findings in diverse syste…”
- Authors
- Bromberg, J. F., Horvath, C. M., Besser, D., Lathem, W. W., & Darnell, J. E.
- Affiliations
- Laboratory of Molecular Cell Biology
- Rockefeller University (US)
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1998-05
- Volume
- 18
- Issue
- 5
- Pages
- 2553-2558
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC110635
- OpenAlex
- W2163046152
- Cited by
- 614
- References
- 2
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 5 · 2021: 5 · 2020: 16 · 2019: 8 · 2018: 9 · 2017: 13 · 2016: 9 · 2015: 16 · 2014: 25 · 2013: 28 · 2012: 24
- FWCI
- 20.39
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- National Institutes of Health (5k08ca067950-02)
- National Cancer Institute (K08 CA67950)
- National Institute of Allergy and Infectious Diseases (R37AI034420, AI 34420, R01AI032489, AI32489)
- Topics
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Keywords
- STAT3, v-Src, cell transformation, oncogenic transformation, v-ras, cytokine signaling, apoptosis inhibition
- MeSH terms
- Animals, Cell Survival, Cell Transformation, Neoplastic, DNA-Binding Proteins, Stem Cells, Trans-Activators, Oncogene Protein pp60(v-src), Gene Expression Regulation, Neoplastic, 3T3 Cells, STAT3 Transcription Factor, Mice
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