CoChat
Sign Up
Cited by 290Fungal and yeast genetics researchMitochondrial Function and PathologyBacterial Genetics and Biotechnology

The DOI matches its Crossref record; 49 of the 50 references it cites were found in a registry or an index.

A Transcriptional Switch in the Expression of Yeast Tricarboxylic Acid Cycle Genes in Response to a Reduction or Loss of Respiratory Function

Open access
Liu, Zhengchang, Butow, Ronald A.·1999·Molecular and Cellular Biology
DOI 10.1128/mcb.19.10.6720PMC PMC84662
Open this paper in CoChat — free account

Abstract

The Hap2,3,4,5p transcription complex is required for expression of many mitochondrial proteins that function in electron transport and the tricarboxylic acid (TCA) cycle. We show that as the cells' respiratory function is reduced or eliminated, the expression of four TCA cycle genes, CIT1, ACO1, IDH1, and IDH2, switches from HAP control to control by three genes, RTG1, RTG2, and RTG3. The expression of four additional TCA cycle genes downstream of IDH1 and IDH2 is independent of the RTG genes. We have previously shown that the RTG genes control the retrograde pathway, defined as a change in the expression of a subset of nuclear genes, e.g., the glyoxylate cycle CIT2 gene, in response to changes in the functional state of mitochondria. We show that the cis-acting sequence controlling RTG-dependent expression of CIT1 includes an R box element, GTCAC, located 70 bp upstream of the Hap2,3,4,5p binding site in the CIT1 upstream activation sequence. The R box is a binding site for Rtg1p-Rtg3p, a heterodimeric, basic helix-loop-helix/leucine zipper transcription factor complex. We propose that in cells with compromised mitochondrial function, the RTG genes take control of the expression of genes leading to the synthesis of alpha-ketoglutarate to ensure that sufficient glutamate is available for biosynthetic processes and that increased flux of the glyoxylate cycle, via elevated CIT2 expression, provides a supply of metabolites entering the TCA cycle sufficient to support anabolic pathways. Glutamate is a potent repressor of RTG-dependent expression of genes encoding both mitochondrial and nonmitochondrial proteins, suggesting that it is a specific feedback regulator of the RTG system.

Overview

Integrity signals

Registry

Crossref · 10.1128/mcb.19.10.6720

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

1 of the 50 references couldn’t be matched to a known paper.

Research works printed but not linked: 6

  1. Chelstowska, A., Z. Liu, Y. Jia, D. Amberg, and R. A. Butow. Yeast, in press.
  2. Kim K. S., 1986, Mol. Cell. Biol., 6, 1936
  3. Liao X., 1991, Mol. Cell. Biol., 11, 38
  4. Lowry C. V., 1988, Mol. Cell. Biol., 8, 4651
  5. Pfeifer K., 1987, Cell, 49, 291
  6. Repetto B., 1989, Mol. Cell. Biol., 9, 2695

Cited by

Sorted by citations

See all 290

Related

See all

Metadata

Authors
Liu, Z., & Butow, R. A.
Affiliations
The University of Texas Southwestern Medical Center (US)
Corresponding author
Butow, Ronald A.
ORCID
Liu, Zhengchang
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1999-10-01
Volume
19
Issue
10
Pages
6720-6728
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.19.10.6720
PMC
PMC84662
OpenAlex
W2102284511
Cited by
290
References
50
Citations by year
2026: 5 · 2025: 4 · 2024: 14 · 2023: 12 · 2022: 7 · 2021: 9 · 2020: 10 · 2019: 7 · 2018: 8 · 2017: 10 · 2016: 7 · 2015: 7 · 2014: 14 · 2013: 17 · 2012: 13
FWCI
4.60
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Welch Foundation
  • National Institutes of Health
  • National Institute of General Medical Sciences (R37GM022525, GM22525)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
transcriptional switch, retrograde signaling, mitochondrial dysfunction, respiratory deficiency, glyoxylate cycle, alpha-ketoglutarate, IDH1, IDH2, ACO1, Saccharomyces cerevisiae
MeSH terms
Aconitate Hydratase, Binding Sites, Citrate (si)-Synthase, Citric Acid Cycle, DNA-Binding Proteins, Fungal Proteins, Genes, Fungal, Genes, Switch, Isocitrate Dehydrogenase, Models, Genetic, Oxygen Consumption, Phenotype, Protein Binding, Saccharomyces cerevisiae, Transcription Factors, Gene Expression Regulation, Fungal, Gene Deletion, Genes, Reporter, Glutamic Acid, Saccharomyces cerevisiae Proteins, Intracellular Signaling Peptides and Proteins, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review