Transcriptional Coregulation by the Cell Integrity Mitogen-Activated Protein Kinase Slt2 and the Cell Cycle Regulator Swi4
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In Saccharomyces cerevisiae, the heterodimeric transcription factor SBF (for SCB binding factor) is composed of Swi4 and Swi6 and activates gene expression at the G(1)/S-phase transition of the mitotic cell cycle. Cell cycle commitment is associated not only with major alterations in gene expression but also with highly polarized cell growth; the mitogen-activated protein kinase (MAPK) Slt2 is required to maintain cell wall integrity during periods of polarized growth and cell wall stress. We describe experiments aimed at defining the regulatory pathway involving the cell cycle transcription factor SBF and Slt2-MAPK. Gene expression assays and chromatin immunoprecipitation experiments revealed Slt2-dependent recruitment of SBF to the promoters of the G(1) cyclins PCL1 and PCL2 after activation of the Slt2-MAPK pathway. We performed DNA microarray analysis and identified other genes whose expression was reduced in both SLT2 and SWI4 deletion strains. Genes that are sensitive to both Slt2 and Swi4 appear to be uniquely regulated and reveal a role for Swi4, the DNA-binding component of SBF, which is independent of the regulatory subunit Swi6. Some of the Swi4- and Slt2-dependent genes do not require Swi6 for either their expression or for Swi4 localization to their promoters. Consistent with these results, we found a direct interaction between Swi4 and Slt2. Our results establish a new Slt2-dependent mode of Swi4 regulation and suggest roles for Swi4 beyond its prominent role in controlling cell cycle transcription.
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In S. cerevisiae, activation of the Pkc1-mediated CWI pathway leads to increased synthesis of β-glucan and enhanced expression of Rlm1 and Swi4 targets including Fks2.
“In S. cerevisiae, activation of the Pkc1-mediated CWI pathway leads to an increased synthesis of -glucan and enhanced expression of Rlm1 and Swi4 targets including Fks2 (49,50).”
From The Rho1 GTPase-activating protein CgBem2 is required for survival of azole stress in Candida glabrata (2011) · cites this paper as [49]
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- Authors
- Baetz, K., Moffat, J., Haynes, J., Chang, M., & Andrews, B.
- Affiliations
- University of Toronto (CA)
- Corresponding author
- Andrews, Brenda
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2001-10
- Volume
- 21
- Issue
- 19
- Pages
- 6515-6528
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC99798
- OpenAlex
- W2154051597
- Cited by
- 125
- References
- 60
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 7 · 2012: 9
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion, other-oa)
- Funders
- Canadian Institutes of Health Research
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Swi4, SBF transcription factor, Swi6, G1/S transition, cell wall integrity, polarized growth, cyclins, transcriptional co-regulation, Saccharomyces cerevisiae
- MeSH terms
- Mating Factor, DNA-Binding Proteins, Fungal Proteins, Models, Genetic, Mutation, Peptides, Promoter Regions, Genetic, RNA, Fungal, Saccharomyces cerevisiae, Transcription Factors, Transcriptional Activation, Gene Expression Regulation, Fungal, G1 Phase, Cyclins, Heat-Shock Response, Oligonucleotide Array Sequence Analysis, Mitogen-Activated Protein Kinases, Saccharomyces cerevisiae Proteins
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