In vivo HP1 targeting causes large-scale chromatin condensation and enhanced histone lysine methylation
Open accessAbstract
Changes in chromatin structure are a key aspect in the epigenetic regulation of gene expression. We have used a lac operator array system to visualize by light microscopy the effect of heterochromatin protein 1 (HP1) alpha (HP1alpha) and HP1beta on large-scale chromatin structure in living mammalian cells. The structure of HP1, containing a chromodomain, a chromoshadow domain, and a hinge domain, allows it to bind to a variety of proteins. In vivo targeting of an enhanced green fluorescent protein-tagged HP1-lac repressor fusion to a lac operator-containing, gene-amplified chromosome region causes local condensation of the higher-order chromatin structure, recruitment of the histone methyltransferase SETDB1, and enhanced trimethylation of histone H3 lysine 9. Polycomb group proteins of both the HPC/HPH and the EED/EZH2 complexes, which are involved in the heritable repression of gene activity, are not recruited to the amplified chromosome region by HP1alpha and HP1beta in vivo targeting. HP1alpha targeting causes the recruitment of endogenous HP1beta to the chromatin region and vice versa, indicating a direct interaction between the two HP1 homologous proteins. Our findings indicate that HP1alpha and HP1beta targeting is sufficient to induce heterochromatin formation.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
HP1 tethering to lacO induces chromatin compaction and silencing.
“HP1 tethering to lacO has been previously shown to induce chromatin compaction and silencing”
From Activation of DNA Damage Response Signaling by Condensed Chromatin (2014) · cites this paper as Verschure et al., 2005
Tethering HP1α, HP1β, HP1γ, or SUV3-9 to lacO arrays induces local chromatin compaction and H3K9me3 enrichment, with reduced nuclease accessibility.
“tethering of these repressive chromatin proteins led to more compact arrays”
From Activation of DNA Damage Response Signaling by Condensed Chromatin (2014) · cites this paper as Verschure et al., 2005
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Jenuwein, T., & Allis, C. D. (2001) · Science · doi:10.1126/science.1063127 · Cited by 9,756 · see citing works
The language of covalent histone modifications
Strahl, B. D., & Allis, C. D. (2000) · Nature · doi:10.1038/47412 · Cited by 8,679 · see citing works
Marr, D., & Hildreth, E. (1980) · Proceedings of the Royal Society of London. Series B. Biological Sciences · doi:10.1098/rspb.1980.0020 · Cited by 6,510 · see citing works
Chen, X., Xiu, M., Xing, J., Yu, S., Min, D., & Guo, F. (2017) · Cellular Physiology and Biochemistry · doi:10.1159/000479439 · Cited by 5,759 · see citing works
Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing
Cao, R., Wang, L., Wang, H., Xia, L., Erdjument-Bromage, H., Tempst, P., Jones, R. S., & Zhang, Y. (2002) · Science · doi:10.1126/science.1076997 · Cited by 3,775 · see citing works
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain
Bannister, A. J., Zegerman, P., Partridge, J. F., Miska, E. A., Thomas, J. O., Allshire, R. C., & Kouzarides, T. (2001) · Nature · doi:10.1038/35065138 · Cited by 2,903 · see citing works
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
Lachner, M., O'Carroll, D., Rea, S., Mechtler, K., & Jenuwein, T. (2001) · Nature · doi:10.1038/35065132 · Cited by 2,868 · see citing works
Role of Histone H3 Lysine 9 Methylation in Epigenetic Control of Heterochromatin Assembly
Nakayama, J.-I., Rice, J. C., Strahl, B. D., Allis, C. D., & Grewal, S. I. S. (2001) · Science · doi:10.1126/science.1060118 · Cited by 1,739 · see citing works
Schultz, D. C., Ayyanathan, K., Negorev, D., Maul, G. G., & Rauscher, F. J. (2002) · Genes & Development · doi:10.1101/gad.973302 · Cited by 1,313 · see citing works
Tachibana, M., Sugimoto, K., Nozaki, M., Ueda, J., Ohta, T., Ohki, M., Fukuda, M., Takeda, N., Niida, H., Kato, H., et al. (2002) · Genes & Development · doi:10.1101/gad.989402 · Cited by 1,261 · see citing works
Partitioning and Plasticity of Repressive Histone Methylation States in Mammalian Chromatin
Peters, A. H. F. M., Kubicek, S., Mechtler, K., O’Sullivan, R. J., Derijck, A. A. H. A., Perez-Burgos, L., Kohlmaier, A., Opravil, S., Tachibana, M., Shinkai, Y., et al. (2003) · Molecular Cell · doi:10.1016/s1097-2765(03)00477-5 · Cited by 1,148 · see citing works
Heterochromatin and Epigenetic Control of Gene Expression
Grewal, S. I. S., & Moazed, D. (2003) · Science · doi:10.1126/science.1086887 · Cited by 1,065 · see citing works
Fischle, W., Wang, Y., Jacobs, S. A., Kim, Y., Allis, C. D., & Khorasanizadeh, S. (2003) · Genes & Development · doi:10.1101/gad.1110503 · Cited by 993 · see citing works
Establishment and Maintenance of a Heterochromatin Domain
Hall, I. M., Shankaranarayana, G. D., Noma, K.-I., Ayoub, N., Cohen, A., & Grewal, S. I. S. (2002) · Science · doi:10.1126/science.1076466 · Cited by 895 · see citing works
Structure of HP1 Chromodomain Bound to a Lysine 9-Methylated Histone H3 Tail
Jacobs, S. A., & Khorasanizadeh, S. (2002) · Science · doi:10.1126/science.1069473 · Cited by 834 · see citing works
The many faces of histone lysine methylation
Lachner, M., & Jenuwein, T. (2002) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(02)00335-6 · Cited by 814 · see citing works
A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G 0 Cells
Ogawa, H., Ishiguro, K., Gaubatz, S., Livingston, D. M., & Nakatani, Y. (2002) · Science · doi:10.1126/science.1069861 · Cited by 769 · see citing works
Transitions in Distinct Histone H3 Methylation Patterns at the Heterochromatin Domain Boundaries
Noma, K.-I., Allis, C. D., & Grewal, S. I. S. (2001) · Science · doi:10.1126/science.1064150 · Cited by 761 · see citing works
Robinett, C. C., Straight, A. F., Li, G., Willhelm, C., Sudlow, G., Murray, A. W., & Belmont, A. S. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.135.6.1685 · Cited by 720 · see citing works
Self-citationFrom Silencing to Gene Expression
Janicki, S. M., Tsukamoto, T., Salghetti, S. E., Tansey, W. P., Sachidanandam, R., Prasanth, K. V., Ried, T., Shav-Tal, Y., Bertrand, E., Singer, R. H., et al. (2004) · Cell · doi:10.1016/s0092-8674(04)00171-0 · Cited by 704 · see citing works
Maison, C., Bailly, D., Peters, A. H. F. M., Quivy, J., Roche, D. M. J., Taddei, A., Lachner, M., Jenuwein, T., & Almouzni, G. (2002) · Nature Genetics · doi:10.1038/ng843 · Cited by 651 · see citing works
HP1 and the dynamics of heterochromatin maintenance
Maison, C., & Almouzni, G. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1355 · Cited by 623 · see citing works
Chambeyron, S., & Bickmore, W. A. (2004) · Genes & Development · doi:10.1101/gad.292104 · Cited by 618 · see citing works
Eissenberg, J. C., James, T. C., Foster-Hartnett, D. M., Hartnett, T., Ngan, V, & Elgin, S. C. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.24.9923 · Cited by 588 · see citing works
Chromatin Higher Order Folding--Wrapping up Transcription
Horn, P. J., & Peterson, C. L. (2002) · Science · doi:10.1126/science.1074200 · Cited by 430 · see citing works
Heterochromatin Formation in Mammalian Cells
Nielsen, A. L., Oulad‐Abdelghani, M., Ortiz, J. A., Remboutsika, E., Chambon, P., & Losson, R. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00218-0 · Cited by 384 · see citing works
Ayyanathan, K., Lechner, M. S., Bell, P., Maul, G. G., Schultz, D., Yamada, Y., Tanaka, K., Torigoe, K., & Rauscher, F. J. (2003) · Genes & Development · doi:10.1101/gad.1102803 · Cited by 356 · see citing works
Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain
Tumbar, T., Sudlow, G., & Belmont, A. S. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.145.7.1341 · Cited by 351 · see citing works
Self-citationWang, H., An, W., Cao, R., Li, X., Erdjument‐Bromage, H., Chatton, B., Tempst, P., Roeder, R. G., & Zhang, Y. (2003) · Molecular Cell · doi:10.1016/j.molcel.2003.08.007 · Cited by 345 · see citing works
Coordinated methyl and RNA binding is required for heterochromatin localization of mammalian HP1α
Muchardt, C., Guillemé, M., Seeler, J.-S., Trouche, D., Dejean, A., & Yaniv, M. (2002) · EMBO Reports · doi:10.1093/embo-reports/kvf194 · Cited by 324 · see citing works
Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells
Minc, E., Allory, Y., Worman, H. J., Courvalin, J., & Buendia, B. (1999) · Chromosoma · doi:10.1007/s004120050372 · Cited by 322 · see citing works
Polycomb complexes and silencing mechanisms
Lund, A. H., & van Lohuizen, M. (2004) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2004.03.010 · Cited by 316 · see citing works
Visualization of gene activity in living cells
Tsukamoto, T., Hashiguchi, N., Janicki, S. M., Tumbar, T., Belmont, A. S., & Spector, D. L. (2000) · Nature Cell Biology · doi:10.1038/35046510 · Cited by 305 · see citing works
Self-citationA sequence motif found in aDrosophilaheterochromatin protein is conserved in animals and plants
Singh, P. B., Miller, J., Pearce, J., Kothary, R., Burton, R. D., Paro, R., James, T. C., & Gaunt, S. J. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.4.789 · Cited by 293 · see citing works
Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals
Cowell, I. G., Aucott, R. L., Mahadevaiah, S. K., Huskisson, N. S., Bongiorni, S., Prantera, G., Fanti, L., Pimpinelli, S., Wu, R., Gilbert, D. M., et al. (2002) · Chromosoma · doi:10.1007/s00412-002-0182-8 · Cited by 262 · see citing works
The chromo shadow domain, a second chromo domain in heterochromatin-binding protein 1, HP1
Assland, R., & stewart, F. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.16.3168 · Cited by 245 · see citing works
WREGGETT, K. A., Hill, F., James, P. S., Hutchings, A., Butcher, G. W., & Singh, P. B. (2008) · Cytogenetics and Cell Genetics · doi:10.1159/000133676 · Cited by 197 · see citing works
RING1 Is Associated with the Polycomb Group Protein Complex and Acts as a Transcriptional Repressor
Satijn, D. P. E., Gunster, M. J., van der Vlag, J., Hamer, K. M., Schul, W., Alkema, M. J., Saurin, A. J., Freemont, P. S., DRIEL, R. V., & Otte, A. P. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.7.4105 · Cited by 189 · see citing works
Müller, W. G., Walker, D. A., Hager, G. L., & McNally, J. G. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.200011069 · Cited by 186 · see citing works
Visualizing chromosome dynamics with GFP
Belmont, A. S. (2001) · Trends in Cell Biology · doi:10.1016/s0962-8924(01)02000-1 · Cited by 169 · see citing works
Self-citationEffects of tethering HP1 to euchromatic regions of the Drosophila genome
Li, Y., Danzer, J. R., Alvarez, P., Belmont, A. S., & Wallrath, L. L. (2003) · Development · doi:10.1242/dev.00405 · Cited by 129 · see citing works
Self-citationA genetic analysis of chromosome territory looping: diverse roles for distal regulatory elements
Ragoczy, T., Telling, A., Sawado, T., Groudine, M. T., & Kosak, S. T. (2003) · Chromosome Research · doi:10.1023/a:1024939130361 · Cited by 123 · see citing works
Festenstein, R., Sharghi-Namini, S., Fox, M., Roderick, K., Tolaini, M., Norton, T., Saveliev, A., Kioussis, D., & Singh, P. B. (1999) · Nature Genetics · doi:10.1038/70579 · Cited by 123 · see citing works
Mechanisms of HP1-mediated gene silencing inDrosophila
Danzer, J. R., & Wallrath, L. L. (2004) · Development · doi:10.1242/dev.01223 · Cited by 108 · see citing works
Generation and Characterization of Methyl-Lysine Histone Antibodies
Perez-Burgos, L., Peters, A. H. F. M., Opravil, S., Kauer, M., Mechtler, K., & Jenuwein, T. (2003) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(03)76016-9 · Cited by 103 · see citing works
Alteration of Large-Scale Chromatin Structure by Estrogen Receptor
Nye, A. C., Rajendran, R. R., Stenoien, D. L., et al. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.10.3437-3449.2002 · Cited by 97 · see citing works
Sewalt, R. G. A. B., Lachner, M., Vargas, M., Hamer, K. M., den Blaauwen, J. L., Hendrix, T., Melcher, M., Schweizer, D., Jenuwein, T., & Otte, A. P. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.15.5539-5553.2002 · Cited by 91 · see citing works
Horsley, D., Hutchings, A., Butcher, G. W., & Singh, P. B. (2008) · Cytogenetics and Cell Genetics · doi:10.1159/000134363 · Cited by 90 · see citing works
Genome Function and Nuclear Architecture: From Gene Expression to NanosciencePreprint
O’Brien, T. P., Bult, C. J., Cremer, C. G., et al. (2003) · Genome Research · doi:10.1101/gr.946403 · Cited by 76 · see citing works
HP1: Facts, open questions, and speculation
Singh, P. B., & Georgatos, S. D. (2002) · Journal of Structural Biology · doi:10.1016/s1047-8477(02)00536-1 · Cited by 74 · see citing works
van der Vlag, J., den Blaauwen, J. L., Sewalt, R. G. A. B., DRIEL, R. V., & Otte, A. P. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.1.697 · Cited by 74 · see citing works
Andersson, G., M�hr, R., Bj�rkroth, B., et al. (1982) · Chromosoma · doi:10.1007/bf00333508 · Cited by 66 · see citing works
Generic Features of Tertiary Chromatin Structure as Detected in Natural Chromosomes
Müller, W. G., Rieder, D., Kreth, G., et al. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.21.9359-9370.2004 · Cited by 53 · see citing works
Verschure, P. J., van der Kraan, I., Enserink, J. M., Moné, M. J., Manders, E. M. M., & DRIEL, R. V. (2002) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215540205001003 · Cited by 43 · see citing works
Self-citationA Panel of Monoclonal Antibodies against Human Polycomb Group Proteins
Hamer, K. M., Sewalt, R. G. A. B., den Blaauwen, J. L., Hendrix, T., Satijn, D. P. E., & Otte, A. P. (2002) · Hybridoma and Hybridomics · doi:10.1089/153685902760213859 · Cited by 32 · see citing works
Activation domain-mediated enhancement of activator binding to chromatin in mammalian cells.
Bunker, C. A., & Kingston, R. E. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.20.10820 · Cited by 25 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Heterochromatin: Guardian of the Genome
Janssen, A., Colmenares, S. U., & Karpen, G. H. (2018) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100617-062653 · Cited by 500 · see citing works
Dynamics and Memory of Heterochromatin in Living Cells
Hathaway, N. A., Bell, O., Hodges, C., Miller, E. L., Neel, D. S., & Crabtree, G. R. (2012) · Cell · doi:10.1016/j.cell.2012.03.052 · Cited by 446 · see citing works
Activation of DNA Damage Response Signaling by Condensed Chromatin
Burgess, R. C., Burman, B., Kruhlak, M. J., & Misteli, T. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.10.060 · Cited by 198 · see citing works
Osterwald, S., Deeg, K. I., Chung, I., Parisotto, D., Wörz, S., Rohr, K., Erfle, H., & Rippe, K. (2015) · Journal of Cell Science · doi:10.1242/jcs.148296 · Cited by 92 · see citing works
Conserved Higher-Order Chromatin Regulates NMDA Receptor Gene Expression and Cognition
Bharadwaj, R., Peter, C. J., Jiang, Y., Roussos, P., Vogel-Ciernia, A., Shen, E. Y., Mitchell, A. C., Mao, W., Whittle, C., Dincer, A., et al. (2014) · Neuron · doi:10.1016/j.neuron.2014.10.032 · Cited by 84 · see citing works
- Authors
- Verschure, P. J., van der Kraan, I., de Leeuw, W., van der Vlag, J., Carpenter, A. E., Belmont, A. S., & van Driel, R.
- Affiliations
- University of Amsterdam (NL)
- Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, University of Amsterdam
- Center for Mathematics and Computer Science, CWI
- Nephrology Research Lab, Nijmegen Center for Molecular Life Sciences, Division of Nephrology, University Medical Center
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Verschure, Pernette J.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2005-06-01
- Volume
- 25
- Issue
- 11
- Pages
- 4552-4564
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC1140641
- OpenAlex
- W2154071588
- Cited by
- 183
- References
- 56
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 5 · 2021: 11 · 2020: 10 · 2019: 9 · 2018: 6 · 2017: 5 · 2016: 7 · 2015: 7 · 2014: 10 · 2013: 8 · 2012: 14
- FWCI
- 6.02
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular and Cellular Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Centrum Wiskunde & Informatica (CWI), the national research institute for mathematics and computer science in the Netherlands (submittedVersion)
- Funders
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- National Institute of General Medical Sciences (R01GM042516)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- HP1, HP1α, HP1β, chromatin condensation, histone H3 lysine 9 trimethylation, SETDB1, Polycomb group proteins, heterochromatin formation, epigenetic silencing, chromatin structure
- MeSH terms
- Chromobox Protein Homolog 5, Cells, Cultured, Chromosomal Proteins, Non-Histone, Chromosomes, Human, Heterochromatin, Histones, Humans, Lysine, Methylation, Operator Regions, Genetic, Histone-Lysine N-Methyltransferase, Protein Methyltransferases, Recombinant Fusion Proteins, Repressor Proteins, Gene Silencing, Green Fluorescent Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
