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Cited by 140Retinal Diseases and TreatmentsRetinal and Macular SurgeryOcular Diseases and Behçet’s Syndrome

Expression of cell adhesion molecules and vascular endothelial growth factor in experimental choroidal neovascularisation in the rat

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Shen, W Y, Yu, M J T, Barry, C J, Constable, I J, Rakoczy, P E·1998·British Journal of Ophthalmology
DOI 10.1136/bjo.82.9.1063PMC PMC1722761
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Abstract

AIMS: To investigate the longevity and reproducibility of choroidal neovascularisation (CNV) induced by krypton laser photocoagulation in the rat. The presence of cell adhesion molecules (CAMs) and vascular endothelial growth factor (VEGF) during the development of CNV was also studied. METHODS: 67 pigmented rats underwent retinal photocoagulation by krypton laser. The eyes were examined by either single or serial fluorescein angiography at 3 days, 1, 2-3, 4-5, 7-8, and 12 weeks post photocoagulation. The expression of CAMs (ICAM-1, E-selectin, and CD44) and VEGF post photocoagulation was studied by immunohistochemistry. RESULTS: CNV related fluorescein leakage appeared in 46.4% of 766 laser spots delivered to the 58 eyes that were tested at 2-3 weeks post treatment. The ratio of hyperfluorescent laser sites did not change significantly at 8 weeks post laser. The number of leaky spots was independent of the total number of lesions delivered to each eye (at 2-3 weeks post laser 10-15 spots/eye: 44% and 25-30 spots/eye: 49%; t = 0.7673; p = 0.3903). Nine eyes were followed by serial angiography between 2 and 12 weeks. The laser spots with fluorescein leakage at 2 weeks (51.5%) remained leaky at 12 weeks (51.5%). Histopathologically, macrophage accumulation peaked at 5 days and CNV was firstly observed at 1 week post photocoagulation. ICAM-1, E-selectin, CD44, and VEGF were maximally induced at 3-5 days post laser photocoagulation, and were localised to RPE, choroidal vascular endothelial, and inflammatory cells. VEGF was also detected in intravascular leucocytes at the sites of laser lesions. CONCLUSIONS: These studies demonstrated that krypton laser photocoagulation can be successfully used to produce lesions similar to those of human CNV. The response induced remained present for an extended period of time (12 weeks), thus offering a potential model to screen candidate CNV inhibitory agents. In addition, it is proposed that the expression of ICAM-1, E-selectin, CD44, and VEGF before new vessel formation might be linked to the initiation of CNV.

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  • Associated inflammation or upregulation of cytokines or molecules other than VEGF in CNV lesions may alter the lesion response to anti-VEGFs.

    “Alternatively, associated inflammation or upregulation of cytokines/molecules other than VEGF in the CNV lesions (for example, HGF, FGF, PDGF, E-Selectin and ICAM-1) [41][42][43][44] may alter the lesion response to anti-VEGFs.”

    From Defining response to anti-VEGF therapies in neovascular AMD (2015) · cites this paper as [41]

    The Discussion (page unavailable) reports inflammatory-cell infiltration and upregulation of ICAM-1 and E-selectin in laser-induced rat CNV lesions. It does not examine anti-VEGF treatment or show that these changes alter lesion response to it. (LLM, conf=0.86)

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Research works printed but not linked: 5

  1. [5]5. Seregard S, Algvere PV, Berglin L. Immunohistochemical characterisation of surgically removed subretinal fibrovas- cular membranes. Graefes Arch Clin Exp Ophthalmol 1994; 232:325-9.
  2. [9]9. Michaelson IC. The model of development of vascular sys- tem of the retina, with some observation on its significance for certain retinal disease. Trans Ophthalmol Soc UK 1948; 68:137-80.
  3. [28]28. Foss AJE, Alexandes RA, JeVeries LW, et al. Immunohisto- chemical techniques: the eVect of melanin bleaching. Br J Biomed Sci 1995;52:22-5.
  4. [30]30. Sunderkotter C, Steinbrink K, Goebeler M, et al. Macro- phages and angiogenesis. J Leukoc Biol 1994;55:410-22. 1070
  5. [31]31. Shen, Yu, Barry, et al on August 16, 2023 by guest. Protected by copyright. http://bjo.bmj.com/ Br J Ophthalmol: first published as 10.1136/bjo.82.9.1063 on 1 September 1998. Downloaded from

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Authors
Shen, W. Y., Yu, M. J. T., Barry, C. J., Constable, I. J., & Rakoczy, P. E.
Affiliations
Lions Eye Institute (AU)
Corresponding author
Rakoczy, P E
ORCID
Venue
British Journal of Ophthalmology
Publisher
BMJ
Published
1998-09-01
Volume
82
Issue
9
Pages
1063-1071
ISSN
0007-1161 (ISSN-L)
DOI
10.1136/bjo.82.9.1063
PMC
PMC1722761
OpenAlex
W2158058021
Cited by
140
References
48
Citations by year
2025: 3 · 2024: 2 · 2023: 3 · 2022: 6 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 5 · 2013: 5 · 2012: 6
FWCI
7.78
Type
journal article
Language
en
Open access
Locations
  • British Journal of Ophthalmology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • UWA Profiles and Research Repository (UWA) (submittedVersion)
  • HighWire Press Open Archive (submittedVersion)
Topics
  • Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Retinal and Macular Surgery
  • Health Sciences › Medicine › Ophthalmology › Ocular Diseases and Behçet’s Syndrome
Keywords
choroidal neovascularization, krypton laser photocoagulation, adhesion molecules, vascular endothelial growth factor, ICAM-1, E-selectin, CD44, retinal pigment epithelium, macrophage accumulation, fluorescein angiography, rat model
MeSH terms
Animals, Choroid, Disease Models, Animal, Fluorescein Angiography, Fundus Oculi, Immunoenzyme Techniques, Lymphokines, Neovascularization, Pathologic, Cell Adhesion Molecules, Endothelial Growth Factors, Laser Coagulation, Hyaluronan Receptors, E-Selectin, Vascular Endothelial Growth Factors, Vascular Endothelial Growth Factor A, Rats

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