Acute haemolysis induced by high dose ascorbic acid in glucose-6-phosphate dehydrogenase deficiency.
Open accessAbstract
We investigated the effects of different neuropeptides on human dendritic cells (DC) maturation. Immature DC, derived from monocytes cultured for 6 days with IL-4 plus GM-CSF, have been exposed to somatostatin, substance P, or vasoactive intestinal peptide (VIP). Among these neuropeptides, only VIP induces the production of bioactive IL-12 and the neoexpression of CD83 on a fraction of the DC population, with an effect significant at 100 and 10 nM, respectively. These effects of VIP are dose-dependent, unaffected by polymixin B, and partly prevented by a VIP receptor antagonist. Although the effects of VIP alone remain modest, it synergizes with TNF-α to induce DC maturation. In the presence of a suboptimal concentration of TNF-α, which has no detectable effect on DC by itself, VIP induces the production of high levels of bioactive IL-12, the neoexpression of CD83 on almost all the DC population (with an effect significant at 10 and 0.1 nM, respectively), and the up-regulation of various adhesion and costimulatory molecule expression. Moreover, DC exposed to VIP plus a suboptimal concentration of TNF-α are as potent as mature DC obtained by treatment with an optimal concentration of TNF-α in stimulating allogenic T cell proliferation. Our data suggest that, in inflammatory sites, VIP may cooperate with proinflammatory mediators, such as TNF-α, to induce DC maturation.
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High-dose intravenous ascorbate caused haemolytic anaemia in patients lacking G6PD.
“Patients with glucose 6-phosphate dehydrogenase (G6PD) deficiency showed haemolytic anaemia following high-dose intravenous ascorbate therapy56–59.”
From Targeting cancer vulnerabilities with high-dose vitamin C (2019) · cites this paper as [56]
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- Authors
- Rees, D. C., Kelsey, H., & Richards, J. D.
- Affiliations
- University College Hospital (GB)
- ORCID
- Rees, D C
- Venue
- BMJ
- Publisher
- BMJ
- Published
- 1993-03-27
- Volume
- 306
- Issue
- 6881
- Pages
- 841-842
- ISSN
- 0007-1447 (ISSN-L)
- 0959-8138
- 1468-5833
- OpenAlex
- W2107149161
- Cited by
- 191
- References
- 12
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 7 · 2023: 2 · 2022: 11 · 2021: 11 · 2020: 71 · 2019: 7 · 2018: 12 · 2017: 4 · 2016: 7 · 2015: 12 · 2014: 5 · 2013: 3 · 2012: 4
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.bmj.com/company/legal-information/terms-conditions/legal-information/tdm-licencepolicy (tdm)
- Locations
- BMJ (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal Health and Biochemistry
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Methemoglobinemia and Tumor Lysis Syndrome
- Health Sciences › Medicine › Hematology › Blood groups and transfusion
- Keywords
- acute hemolysis, glucose-6-phosphate dehydrogenase deficiency, vitamin C, vitamin C toxicity
- MeSH terms
- Acute Disease, Adult, Ascorbic Acid, Glucosephosphate Dehydrogenase Deficiency, Hemolysis, Humans, Male
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