Cited by 384Gut microbiota and healthInflammatory Bowel DiseaseMicroscopic Colitis

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Gut-associated bacterial microbiota in paediatric patients with inflammatory bowel disease

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Conte, M P, Schippa, S, Zamboni, I, Penta, M, Chiarini, F, Seganti, L, Osborn, J, Falconieri, P, Borrelli, O, Cucchiara, S·2006·Gut

DOI 10.1136/gut.2005.078824PMC PMC1856471

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Abstract

Background: Clinical and experimental observations in animal models indicate that intestinal commensal bacteria are involved in the initiation and amplification of inflammatory bowel disease (IBD). No paediatric reports are available on intestinal endogenous microflora in IBD. Aims: To investigate and characterise the predominant composition of the mucosa-associated intestinal microflora in colonoscopic biopsy specimens of paediatric patients with newly diagnosed IBD. Methods: Mucosa-associated bacteria were quantified and isolated from biopsy specimens of the ileum, caecum and rectum obtained at colonoscopy in 12 patients with Crohn’s disease, 7 with ulcerative colitis, 6 with indeterminate colitis, 10 with lymphonodular hyperplasia of the distal ileum and in 7 controls. Isolation and characterisation were carried out by conventional culture techniques for aerobic and facultative-anaerobic microorganisms, and molecular analysis (16S rRNA-based amplification and real-time polymerase chain reaction assays) for the detection of anaerobic bacterial groups or species. Results: A higher number of mucosa-associated aerobic and facultative-anaerobic bacteria were found in biopsy specimens of children with IBD than in controls. An overall decrease in some bacterial species or groups belonging to the normal anaerobic intestinal flora was suggested by molecular approaches; in particular, occurrence of Bacteroides vulgatus was low in Crohn’s disease, ulcerative colitis and indeterminate colitis specimens. Conclusion: This is the first paediatric report investigating the intestinal mucosa-associated microflora in patients of the IBD spectrum. These results, although limited by the sample size, allow a better understanding of changes in mucosa-associated bacterial flora in these patients, showing either a predominance of some potentially harmful bacterial groups or a decrease in beneficial bacterial species. These data underline the central role of mucosa-adherent bacteria in IBD.

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Authors: Conte, M. P., Schippa, S., Zamboni, I., Penta, M., Chiarini, F., Seganti, L., Osborn, J., Falconieri, P., Borrelli, O., & Cucchiara, S.
Affiliations: Policlinico Umberto I (IT)
Corresponding author: Cucchiara, S
ORCID: Conte, M P
Schippa, S
Borrelli, O
Cucchiara, S
Venue: Gut
Publisher: BMJ
Published: 2006-04-28
Volume: 55
Issue: 12
Pages: 1760-1767
ISSN: 0017-5749 (ISSN-L)
1468-3288
DOI: 10.1136/gut.2005.078824
PMC: PMC1856471
OpenAlex: W2152822836
Cited by: 383
References: 51
Citations by year: 2026: 4 · 2025: 7 · 2024: 8 · 2023: 15 · 2022: 14 · 2021: 23 · 2020: 13 · 2019: 23 · 2018: 12 · 2017: 17 · 2016: 19 · 2015: 25 · 2014: 27 · 2013: 23 · 2012: 20
FWCI: 9.51
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Gut (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
Funders: Ministero dell’Istruzione, dell’Università e della Ricerca
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
Health Sciences › Medicine › Gastroenterology › Microscopic Colitis
Keywords: inflammatory bowel disease, pediatric patients, gut microbiota, mucosa-associated bacteria, Crohn's disease, ulcerative colitis, indeterminate colitis, Bacteroides vulgatus, anaerobic bacteria, colonoscopic biopsy, 16S rRNA
MeSH terms: Adolescent, Bacteria, Aerobic, Bacteria, Anaerobic, Bacteroides, Cecum, Child, Child, Preschool, Colitis, Colitis, Ulcerative, Crohn Disease, DNA, Bacterial, Humans, Hyperplasia, Ileum, Intestinal Mucosa, Rectum, Inflammatory Bowel Diseases, Polymerase Chain Reaction

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Gut-associated bacterial microbiota in paediatric patients with inflammatory bowel disease

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