The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Photocontrollable Fluorescent Proteins for Superresolution Imaging
Open accessAbstract
Superresolution fluorescence microscopy permits the study of biological processes at scales small enough to visualize fine subcellular structures that are unresolvable by traditional diffraction-limited light microscopy. Many superresolution techniques, including those applicable to live cell imaging, utilize genetically encoded photocontrollable fluorescent proteins. The fluorescence of these proteins can be controlled by light of specific wavelengths. In this review, we discuss the biochemical and photophysical properties of photocontrollable fluorescent proteins that are relevant to their use in superresolution microscopy. We then describe the recently developed photoactivatable, photoswitchable, and reversibly photoswitchable fluorescent proteins, and we detail their particular usefulness in single-molecule localization–based and nonlinear ensemble–based superresolution techniques. Finally, we discuss recent applications of photocontrollable proteins in superresolution imaging, as well as how these applications help to clarify properties of intracellular structures and processes that are relevant to cell and developmental biology, neuroscience, cancer biology and biomedicine.
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Cites
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
Grotjohann, T., Testa, I., Leutenegger, M., Bock, H., Urban, N. T., Lavoie-Cardinal, F., Willig, K. I., Eggeling, C., Jakobs, S., & Hell, S. W. (2011) · Nature · doi:10.1038/nature10497 · Cited by 501
Nanoscopy of living brain slices with low light levels
Testa, I., Urban, N. T., Jakobs, S., Eggeling, C., Willig, K. I., & Hell, S. W. (2012) · Neuron · doi:10.1016/j.neuron.2012.07.028 · Cited by 131
Cited by
Single-molecule localization microscopy
Lelek, M., Gyparaki, M. T., Beliu, G., Schueder, F., Griffié, J., Manley, S., Jungmann, R., Sauer, M., Lakadamyali, M., & Zimmer, C. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-021-00038-x · Cited by 954
A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging
Specht, E. A., Braselmann, E., & Palmer, A. E. (2017) · Annual Review of Physiology · doi:10.1146/annurev-physiol-022516-034055 · Cited by 444
Stimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
Switchable Fluorophores for Single-Molecule Localization Microscopy
Li, H., & Vaughan, J. C. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00767 · Cited by 319
Barriers in the brain: resolving dendritic spine morphology and compartmentalization
Adrian, M., Kusters, R., Wierenga, C. J., Storm, C., Hoogenraad, C. C., & Kapitein, L. C. (2014) · Frontiers in Neuroanatomy · doi:10.3389/fnana.2014.00142 · Cited by 62
“Novel probes are currently being developed that should allow two-color RESOLFT of dendritic spines (Lavoie-Cardinal et al., 2014; Shcherbakova et al., 2014).”
Metadata
- Authors
- Shcherbakova, D. M., Sengupta, P., Lippincott-Schwartz, J., & Verkhusha, V. V.
- Venue
- Annual Review of Biophysics
- Publisher
- Annual Reviews
- Published
- 2014-05-06
- Volume
- 43
- Issue
- 1
- Pages
- 303-329
- ISSN
- 1936-122X (ISSN-L)
- 1936-1238
- OpenAlex
- W2145157070
- Cited by
- 228
- References
- 4
- FWCI
- 3.40
- Type
- journal article
- Language
- en
- Open access
- bronze
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