The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Design and Selection of Novel Cys2His2 Zinc Finger Proteins
Abstract
▪ Abstract Cys 2 His 2 zinc finger proteins offer a stable and versatile framework for the design of proteins that recognize desired target sites on double-stranded DNA. Individual fingers from these proteins have a simple ββα structure that folds around a central zinc ion, and tandem sets of fingers can contact neighboring subsites of 3–4 base pairs along the major groove of the DNA. Although there is no simple, general code for zinc finger–DNA recognition, selection strategies have been developed that allow these proteins to be targeted to almost any desired site on double-stranded DNA. The affinity and specificity of these new proteins can also be improved by linking more fingers together or by designing proteins that bind as dimers and thus recognize an extended site. These new proteins can then be modified by adding other domains—for activation or repression of transcription, for DNA cleavage, or for other activities. Such designer transcription factors and other new proteins will have important applications in biomedical research and in gene therapy.
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Citations
Cites
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Comparative Genomics of the Eukaryotes
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Crystal Structure of a Five-Finger GLI-DNA Complex: New Perspectives on Zinc Fingers
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Self-citationHöller, M., Westin, G., Jiricny, J., & Schaffner, W. (1988) · Genes & Development · doi:10.1101/gad.2.9.1127 · Cited by 305
In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence
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Structure-Based Design of Transcription Factors
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Cited by
TALENs: A widely applicable technology for targeted genome editing
Joung, J. K., & Sander, J. D. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3486 · Cited by 1,650
“83, 84 Engineered zinc fingers can be joined together into more extended arrays capable of recognizing longer DNA sequences.”
PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice
Baudat, F., Buard, J., Grey, C., Fledel-Alon, A., Ober, C., Przeworski, M., Coop, G., & de Massy, B. (2010) · Science · doi:10.1126/science.1183439 · Cited by 1,026
An improved zinc-finger nuclease architecture for highly specific genome editing
Miller, J. C., Holmes, M. C., Wang, J., Guschin, D. Y., Lee, Y.-L., Rupniewski, I., Beausejour, C. M., Waite, A. J., Wang, N. S., Kim, K. A., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1319 · Cited by 1,051
Self-citationKnockout Rats via Embryo Microinjection of Zinc-Finger Nucleases
Geurts, A. M., Cost, G. J., Freyvert, Y., Zeitler, B., Miller, J. C., Choi, V. M., Jenkins, S. S., Wood, A., Cui, X., Meng, X., et al. (2009) · Science · doi:10.1126/science.1172447 · Cited by 934
Targeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases
Bibikova, M., Golic, M., Golic, K. G., & Carroll, D. (2002) · Genetics · doi:10.1093/genetics/161.3.1169 · Cited by 860
Metadata
- Authors
- Pabo, C. O., Peisach, E., & Grant, R. A.
- Venue
- Annual Review of Biochemistry
- Publisher
- Annual Reviews
- Published
- 2001-06
- Volume
- 70
- Issue
- 1
- Pages
- 313-340
- ISSN
- 0066-4154 (ISSN-L)
- 1545-4509
- OpenAlex
- W2132256310
- Cited by
- 715
- References
- 8
- FWCI
- 1.67
- Type
- journal article
- Language
- en
- Open access
- closed
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