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Cited by 166Muscle metabolism and nutritionMuscle Physiology and DisordersCardiovascular and exercise physiology

The DOI matches its Crossref record; 41 of the 42 references it cites were found in a registry or an index.

Anabolic signaling and protein synthesis in human skeletal muscle after dynamic shortening or lengthening exercise

Cuthbertson, Daniel J., Babraj, John, Smith, Kenneth, Wilkes, Emilie, Fedele, Mark J., Esser, Karyn, Rennie, Michael·2006·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00415.2005
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Abstract

We hypothesized a differential activation of the anabolic signaling proteins protein kinase B (PKB) and p70 S6 kinase (p70S6K) and subsequent differential stimulation of human muscle protein synthesis (MPS) after dynamic shortening or lengthening exercise. Eight healthy men [25 ± 5 yr, BMI 26 ± 3 kg/m−2 (means ± SD)] were studied before and after 12 min of repeated stepping up to knee height, and down again, while carrying 25% of their body weight, i.e., shortening exercise with the “up” leg and lengthening exercise with contralateral “down” leg. Quadriceps biopsies were taken before and 3, 6, and 24 h after exercise. After exercise, over 2 h before the biopsies, the subjects ingested 500 ml of water containing 45 g of essential amino acids and 135 g of sucrose. Rates of muscle protein synthesis were determined via incorporation over time of [1-13C]leucine (≤6 h after exercise) or [1-13C]valine (21–24 h after exercise) and phosphorylation of signaling proteins by Western analysis. PKB and p70S6K phosphorylation increased ∼3-fold after 3 h and remained elevated at 6 and 24 h. After exercise, rates of myofibrillar and sarcoplasmic protein synthesis were unchanged over the period including exercise and 3 h of recovery but had increased significantly at 6 (∼3.0- and 2.4-fold, respectively) and 24 h (∼3.2- and 2.0-fold, respectively), independently of the mode of exercise. Short-term dynamic exercise in either shortening or lengthening mode increases MPS at least as much as resistance exercise and is associated with long-term activation of PKB and p70S6K.

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Metadata

Authors
Cuthbertson, D. J., Babraj, J., Smith, K., Wilkes, E., Fedele, M. J., Esser, K., & Rennie, M.
Affiliations
  • Ninewells Hospital (GB)
  • University of Dundee (GB)
  • University of Nottingham (GB)
  • University of Illinois Urbana-Champaign (US)
  • University of Kentucky (US)
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2006-04
Volume
290
Issue
4
Pages
E731-E738
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00415.2005
OpenAlex
W2135780085
Cited by
166
References
42
Citations by year
2026: 3 · 2025: 3 · 2024: 5 · 2023: 8 · 2022: 6 · 2021: 7 · 2020: 6 · 2019: 4 · 2018: 7 · 2017: 12 · 2016: 10 · 2015: 13 · 2014: 11 · 2013: 9 · 2012: 7
FWCI
3.66
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Wellcome Trust
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR 45617, R01AR049869, AR49869, R01AR045617)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
Keywords
muscle protein synthesis, anabolic signaling, Akt, p70 S6 kinase, dynamic exercise, quadriceps, myofibrillar protein synthesis, leucine incorporation, post-exercise recovery, resistance training
MeSH terms
Adult, Biopsy, Collagen, Humans, Leucine, Male, Muscle Proteins, Myofibrils, Phosphorylation, Random Allocation, Sarcoplasmic Reticulum, Valine, Blotting, Western, Signal Transduction, Exercise, Ribosomal Protein S6 Kinases, 70-kDa, Proto-Oncogene Proteins c-akt, Quadriceps Muscle

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