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Cited by 54Muscle metabolism and nutritionCardiovascular and exercise physiologySports Performance and Training

Impact of protein coingestion on muscle protein synthesis during continuous endurance type exercise

Beelen, Milou, Zorenc, Antoine, Pennings, Bart, Senden, Joan M., Kuipers, Harm, van Loon, Luc J. C.·2011·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00446.2010
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Abstract

This study investigates the impact of protein coingestion with carbohydrate on muscle protein synthesis during endurance type exercise. Twelve healthy male cyclists were studied during 2 h of fasted rest followed by 2 h of continuous cycling at 55% W max. During exercise, subjects received either 1.0 g·kg−1·h−1 carbohydrate (CHO) or 0.8 g·kg−1·h−1 carbohydrate with 0.2 g·kg−1·h−1 protein hydrolysate (CHO+PRO). Continuous intravenous infusions with l-[ ring-13 C 6]phenylalanine and l-[ ring-2 H 2]tyrosine were applied, and blood and muscle biopsies were collected to assess whole body protein turnover and muscle protein synthesis rates at rest and during exercise conditions. Protein coingestion stimulated whole body protein synthesis and oxidation rates during exercise by 22 ± 3 and 70 ± 17%, respectively ( P < 0.01). Whole body protein breakdown rates did not differ between experiments. As a consequence, whole body net protein balance was slightly negative in CHO and positive in the CHO+PRO treatment (−4.9 ± 0.3 vs. 8.0 ± 0.3 μmol Phe·kg−1·h−1, respectively, P < 0.01). Mixed muscle protein fractional synthetic rates (FSR) were higher during exercise compared with resting conditions (0.058 ± 0.006 vs. 0.035 ± 0.006%/h in CHO and 0.070 ± 0.011 vs. 0.038 ± 0.005%/h in the CHO+PRO treatment, respectively, P < 0.05). FSR during exercise did not differ between experiments ( P = 0.46). We conclude that muscle protein synthesis is stimulated during continuous endurance type exercise activities when carbohydrate with or without protein is ingested. Protein coingestion does not further increase muscle protein synthesis rates during continuous endurance type exercise.

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  • Adding protein to carbohydrate consumption throughout a prolonged bout of endurance exercise does not exert any further impact on rates of MPS.

    “but the added protein does not exert any further impact on rates of MPS.”

    From International Society of Sports Nutrition Position Stand: protein and exercise (2017) · cites this paper as [14]

  • Adding protein to carbohydrate consumption throughout a prolonged bout of endurance exercise promotes a higher whole body net protein balance.

    “Beelen and colleagues [14] determined that adding protein to carbohydrate consumption throughout a prolonged bout of endurance exercise promotes a higher whole body net protein balance”

    From International Society of Sports Nutrition Position Stand: protein and exercise (2017) · cites this paper as [14]

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Authors
Beelen, M., Zorenc, A., Pennings, B., Senden, J. M., Kuipers, H., & van Loon, L. J. C.
Affiliations
  • Maastricht University Medical Centre (NL)
  • Department of Human Movement Sciences, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre+, Maastricht, the Netherlands
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2011-06
Volume
300
Issue
6
Pages
E945-E954
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00446.2010
OpenAlex
W2068099348
Cited by
54
References
51
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 5 · 2015: 4 · 2014: 5 · 2013: 10 · 2012: 5
FWCI
3.21
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion)
  • Research Bank (Australian Catholic University) (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
Keywords
muscle protein synthesis, endurance exercise, carbohydrate intake, protein hydrolysates, whole-body protein turnover, net protein balance, fractional synthesis rate, protein oxidation
MeSH terms
Amino Acids, Beverages, Bicycling, Biopsy, Diet, Dietary Carbohydrates, Dietary Proteins, Humans, Male, Muscle Proteins, Phenylalanine, Physical Endurance, Tyrosine, Cyclic AMP-Dependent Protein Kinases, Muscle, Skeletal, Lactic Acid, Young Adult, TOR Serine-Threonine Kinases

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