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Cited by 162Muscle metabolism and nutritionNutrition and Health in AgingMuscle Physiology and Disorders

The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.

Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise

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Bukhari, Syed S. I., Phillips, Bethan E., Wilkinson, Daniel J., Limb, Marie C., Rankin, Debbie, Mitchell, William K., Kobayashi, Hisamine, Greenhaff, Paul L., Smith, Kenneth, Atherton, Philip J.·2015·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00481.2014
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Abstract

Dysregulated anabolic responses to nutrition/exercise may contribute to sarcopenia; however, these characteristics are poorly defined in female populations. We determined the effects of two feeding regimes in older women (66 ± 2.5 yr; n = 8/group): bolus whey protein (WP-20 g) or novel low-dose leucine-enriched essential amino acids (EAA) [LEAA; 3 g (40% leucine)]. Using [ 13 C 6 ]phenylalanine infusions, we quantified muscle (MPS) and albumin (APS) protein synthesis at baseline and in response to both feeding (FED) and feeding plus exercise (FED-EX; 6 × 8 knee extensions at 75% 1-repetition maximum). We also quantified plasma insulin/AA concentrations, whole leg (LBF)/muscle microvascular blood flow (MBF), and muscle anabolic signaling by phosphoimmunoblotting. Plasma insulinemia and EAA/aemia were markedly greater after WP than LEAA ( P < 0.001). Neither LEAA nor WP modified LBF in response to FED or FED-EX, whereas MBF increased to a similar extent in both groups only after FED-EX ( P < 0.05). In response to FED, both WP and LEAA equally stimulated MPS 0–2 h ( P < 0.05), abating thereafter (0–4 h, P > 0.05). In contrast, after FED-EX, MPS increased at 0–2 h and remained elevated at 0–4 h ( P < 0.05) with both WP and LEAA. No anabolic signals quantifiably increased after FED, but p70 S6K1 Thr 389 increased after FED-EX (2 h, P < 0.05). APS increased similarly after WP and LEAA. Older women remain subtly responsive to nutrition ± exercise. Intriguingly though, bolus WP offers no trophic advantage over LEAA.

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Crossref · 10.1152/ajpendo.00481.2014

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  1. Häkkinen K, 2001, Acta Physiol Scand, 171, 51

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Metadata

Authors
Bukhari, S. S. I., Phillips, B. E., Wilkinson, D. J., Limb, M. C., Rankin, D., Mitchell, W. K., Kobayashi, H., Greenhaff, P. L., Smith, K., & Atherton, P. J.
Affiliations
  • University of Nottingham (GB)
  • Ajinomoto (Japan) (JP)
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2015-06-15
Volume
308
Issue
12
Pages
E1056-E1065
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00481.2014
OpenAlex
W2141893506
Cited by
162
References
63
Citations by year
2026: 11 · 2025: 17 · 2024: 12 · 2023: 11 · 2022: 19 · 2021: 10 · 2020: 24 · 2019: 11 · 2018: 16 · 2017: 12 · 2016: 15 · 2015: 4
FWCI
8.34
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
  • Repository@Nottingham (University of Nottingham) (submittedVersion)
Funders
Medical Research Council (MR/K00414X/1)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Physiology (medical) › Nutrition and Health in Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
whey protein, muscle protein synthesis, sarcopenia, older women, physical activity, muscle anabolism, insulin, anabolic signaling, essential amino acids
MeSH terms
Whey Proteins, Aged, Amino Acids, Essential, Diet, Dietary Proteins, Female, Humans, Insulin, Leucine, Middle Aged, Milk Proteins, Muscle Proteins, Rest, Exercise, Muscle, Skeletal, Dietary Supplements

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