Cited by 53Muscle Physiology and DisordersFatty Acid Research and HealthNutrition and Health in Aging

Eicosapentaenoic Acid Attenuates Arthritis-Induced Muscle Wasting Acting on Atrogin-1 and on Myogenic Regulatory Factors

Castillero, Estíbaliz, Martín, Ana Isabel, López-Menduiña, María, Villanúa, Maria Angeles, López-Calderón, Asunción·2009·American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

Abstract

Eicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid that has anti-inflammatory and anticachectic actions. The aim of this work was to elucidate whether EPA administration is able to prevent an arthritis-induced decrease in body weight and muscle wasting in rats. Arthritis was induced by intradermal injection of Freund's adjuvant; 3 days later, nine rats received 1 g/kg EPA or coconut oil daily. All rats were killed 15 days after adjuvant injection. EPA administration decreased the external signs of arthritis and paw volume as well as liver TNF-alpha mRNA. EPA did not modify arthritis-induced decrease in food intake or body weight gain. However, EPA treatment prevented arthritis-induced increase in muscle TNF-alpha and atrogin-1, whereas it attenuated the decrease in gastrocnemius weight and the increase in MuRF1 mRNA. Arthritis not only decreased myogenic regulatory factors but also increased PCNA, MyoD, and myogenin mRNA in the gastrocnemius. Western blot analysis showed that changes in protein content followed the pattern seen with mRNA. In the control rats, EPA administration increased PCNA and MyoD mRNA and protein. In arthritic rats, EPA did not modify the stimulatory effect of arthritis on these myogenic regulatory factors. The results suggest that in experimental arthritis, in addition to its anti-inflammatory effect, EPA treatment attenuates muscle wasting by decreasing atrogin-1 and MuRF1 gene expression and increasing the transcription factors that regulate myogenesis.

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Crossref · 10.1152/ajpregu.00388.2009

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  • Oral administration of eicosapentaenoic acid attenuates arthritis-induced muscle wasting in Wistar rats by decreasing MAFbx and MuRF1 and increasing PCNA, MyoD, and myogenin.

    “Oral administration of eicosapentaenoic acid attenuates arthritis-induced muscle wasting in Wistar rats by decreasing the expression of MAFbx and MuRF1 and increasing the expression of proliferating cell nuclear antigen, MyoD, and myogenin [75].”

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Authors
Castillero, E., Martín, A. I., López-Menduiña, M., Villanúa, M. A., & López-Calderón, A.
Affiliations
  • Universidad Complutense de Madrid (ES)
  • Department of Physiology, Faculty of Medicine, Complutense University of Madrid, Madrid, Spain
Venue
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
Published
2009-11
Volume
297
Issue
5
Pages
R1322-R1331
ISSN
  • 0363-6119 (ISSN-L)
  • 1522-1490
OpenAlex
W2137737198
Cited by
53
References
53
Citations by year
2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 3 · 2017: 5 · 2016: 5 · 2015: 1 · 2014: 3 · 2013: 3 · 2012: 2
FWCI
1.61
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
Keywords
eicosapentaenoic acid, omega-3 polyunsaturated fatty acids, arthritis, muscle atrophy, atrogin-1, MuRF1, myogenic regulatory factors, MyoD, myogenin, PCNA, TNF-α, gastrocnemius, complete Freund's adjuvant-induced arthritis, cachexia, myogenesis
MeSH terms
Tripartite Motif Proteins, Animals, Arthritis, Experimental, Arthritis, Rheumatoid, Cell Differentiation, Disease Models, Animal, Male, Muscle Proteins, Tumor Necrosis Factor-alpha, Eicosapentaenoic Acid, Rats, Wistar, MyoD Protein, Myogenic Regulatory Factors, Myogenin, Muscle, Skeletal, Proliferating Cell Nuclear Antigen, Wasting Syndrome, Ubiquitin-Protein Ligases, SKP Cullin F-Box Protein Ligases, Cell Proliferation, Rats

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