Nuclear factor-κB-like activity increases in murine cerebral cortex after sleep deprivation
Abstract
Several well-defined sleep regulatory substances, e.g., interleukin-1β, activate the heterodimeric transcription factor nuclear factor-κB (NF-κB). Several substances that inhibit sleep, e.g., interleukin-4, inhibit NF-κB activation. NF-κB activation promotes production of several additional substances thought to be involved in sleep regulation, e.g., nitric oxide. We investigated, therefore, whether there are diurnal rhythms of NF-κB activation in brain and changes in the activation after sleep deprivation. Mice were kept on a 12:12-h light-dark cycle. In one experiment, groups of mice were killed every 3 h across the 24-h cycle. In another experiment, mice were killed at 1500 after 6 h of sleep deprivation, and a group of control mice were killed at the same time. Nuclear proteins were extracted from each brain tissue sample, and NF-κB-like activity was determined with an electrophoretic mobility shift assay. In cerebral cortex, but not other areas of brain, there was a diurnal rhythm in NF-κB-like activation; highest levels were found during the light period. NF-κB-like activation was higher in cerebral cortex after sleep deprivation compared with values obtained from control mice. The results are consistent with the hypothesis that sleep regulation involves multiple gene events, some of which include enhanced production of sleep regulatory substances, the actions of which involve NF-κB activation.
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Sleep deprivation raises NF-κB in the murine cerebral cortex.
“Consistent with this observation, sleep deprivation increases murine cerebral cortex NF-κB (44).”
From Oxalic acid and diacylglycerol 36:3 are cross-species markers of sleep debt (2015) · cites this paper as [44]
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- Authors
- Chen, Z., Gardi, J., Kushikata, T., Fang, J., & Krueger, J. M.
- Affiliations
- Washington State University (US)
- Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164
- ORCID
- Kushikata, Tetsuya
- Venue
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Publisher
- American Physiological Society
- Published
- 1999-06-01
- Volume
- 276
- Issue
- 6
- Pages
- R1812-R1818
- ISSN
- 0363-6119 (ISSN-L)
- 1522-1490
- OpenAlex
- W2103918039
- Cited by
- 78
- References
- 45
- Citations by year
- 2026: 1 · 2024: 6 · 2023: 4 · 2022: 5 · 2021: 4 · 2020: 2 · 2019: 2 · 2016: 4 · 2015: 3 · 2014: 1 · 2012: 3
- FWCI
- 2.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS-31453, NS-25378, R01NS031453, R01NS025378)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-36520, R01HD036520)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Keywords
- nuclear factor-kappaB, sleep deprivation, cerebral cortex, diurnal cycle, sleep regulation, IL-1β, IL-4, nitric oxide, transcription factor activation, light-dark cycle
- MeSH terms
- Animals, Cerebral Cortex, Circadian Rhythm, Electrophoresis, Light, Male, Mice, Inbred Strains, Reference Values, Sleep Deprivation, NF-kappa B, Mice
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