The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Reversal of charge selectivity in cation or anion-selective epithelial lines by expression of different claudins
Abstract
Tight junctions (TJ) regulate paracellular ionic charge selectivity and conductance across epithelial tissues and cell lines. These properties vary among epithelia, and recent evidence implicates the claudins, a family of TJ transmembrane proteins, as important determinants of both characteristics. To test the hypothesis that each claudin contributes a characteristic charge discrimination to the TJ, we expressed claudins-2, -4, -11, and -15 in both cation-selective Madin-Darby canine kidney (MDCK) II cells and in anion-selective LLC-PK 1 cells and examined changes in transepithelial electrical resistance (TER) and paracellular charge selectivity. Regulated expression and localization were verified by immunoblot analysis and immunofluorescence microscopy, respectively. Expression of claudin-4 increased TER in both cell lines, whereas effects of the others on TER were variable. Claudin-4 and -11 decreased paracellular permeability for Na+in MDCK II cells, whereas neither claudin-2 nor -15 had an effect. Conversely, in LLC-PK 1 cells, claudin-2 and -15 increased the permeability for Na+, whereas claudin-4 and -11 were without effect. We conclude that the contribution of each claudin is most easily detectable when it reverses the direction of monolayer charge selectivity. These results are consistent with a model in which exogenous claudins add new charge-selective pores, leading to a physiological phenotype that combines endogenous and exogenous contributions. Additionally, it is possible to rationalize the direction of charge selectivity conferred by the individual claudins on the basis of electrostatic effects of the charged amino acids in their first extracellular loops.
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González-Mariscal, L., Betanzos, A., Nava, P., & Jaramillo, B. E. (2003) · Progress in Biophysics and Molecular Biology · doi:10.1016/s0079-6107(02)00037-8 · Cited by 1,160
Furuse, M., Sasaki, H., Fujimoto, K., & Tsukita, S. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.2.391 · Cited by 965
Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells
Amasheh, S., Meiri, N., Gitter, A. H., Schöneberg, T., Mankertz, J., Schulzke, J. D., & Fromm, M. (2002) · Journal of Cell Science · doi:10.1242/jcs.00165 · Cited by 812
Manner of Interaction of Heterogeneous Claudin Species within and between Tight Junction Strands
Furuse, M., Sasaki, H., & Tsukita, S. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.4.891 · Cited by 746
Furuse, M., Furuse, K., Sasaki, H., & Tsukita, S. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.2.263 · Cited by 719
CNS Myelin and Sertoli Cell Tight Junction Strands Are Absent in Osp/Claudin-11 Null Mice
Gow, A., Southwood, C. M., Li, J. S., Pariali, M., Riordan, G. P., Brodie, S. E., Danias, J., Bronstein, J. M., Kachar, B., & Lazzarini, R. A. (1999) · Cell · doi:10.1016/s0092-8674(00)81553-6 · Cited by 699
Powell, D. W. (1981) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1981.241.4.g275 · Cited by 622
Van Itallie, C., Rahner, C., & Anderson, J. M. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12464 · Cited by 601
Self-citationClaude, P. (1978) · The Journal of Membrane Biology · doi:10.1007/bf01870332 · Cited by 565
Rahner, C., Mitic, L. L., & Anderson, J. M. (2001) · Gastroenterology · doi:10.1053/gast.2001.21736 · Cited by 546
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Cited by
Claudins and the Modulation of Tight Junction Permeability
Günzel, D., & Yu, A. S. L. (2013) · Physiological Reviews · doi:10.1152/physrev.00019.2012 · Cited by 1,456
Physiology and function of the tight junction
Anderson, J. M., & Van Itallie, C. M. (2009) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a002584 · Cited by 1,059
Self-citationTight Junction Pore and Leak Pathways: A Dynamic Duo
Shen, L., Weber, C. R., Raleigh, D. R., Yu, D., & Turner, J. R. (2011) · Annual Review of Physiology · doi:10.1146/annurev-physiol-012110-142150 · Cited by 872
Structure and function of claudins
Krause, G., Winkler, L., Mueller, S. L., Haseloff, R. F., Piontek, J., & Blasig, I. E. (2008) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2007.10.018 · Cited by 829
Cytokine regulation of tight junctions
Capaldo, C. T., & Nusrat, A. (2009) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2008.08.027 · Cited by 718
Metadata
- Authors
- Van Itallie, C. M., Fanning, A. S., & Anderson, J. M.
- Venue
- American Journal of Physiology-Renal Physiology
- Publisher
- American Physiological Society
- Published
- 2003-12
- Volume
- 285
- Issue
- 6
- Pages
- F1078-F1084
- ISSN
- 1522-1466 (ISSN-L)
- 1931-857X
- OpenAlex
- W2132226772
- Cited by
- 371
- References
- 24
- FWCI
- 10.49
- Type
- journal article
- Language
- en
- Open access
- closed
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- Van Itallie, C. M., Fanning, A. S., & Anderson, J. M. (2003). Reversal of charge selectivity in cation or anion-selective epithelial lines by expression of different claudins. American Journal of Physiology-Renal Physiology. https://doi.org/10.1152/ajprenal.00116.2003
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