CoChat
Sign Up
Cited by 288Cardiovascular and exercise physiologyMuscle metabolism and nutritionAdvanced MRI Techniques and Applications

The DOI matches its Crossref record; 27 of the 28 references it cites were found in a registry or an index.

Relationships between in vivo and in vitro measurements of metabolism in young and old human calf muscles

McCully, K. K., Fielding, R. A., Evans, W. J., Leigh, J. S., Posner, J. D.·1993·Journal of Applied Physiology
DOI 10.1152/jappl.1993.75.2.813
Open this paper in CoChat — free account

Abstract

This study compared in vivo measurements of muscle metabolism in humans with magnetic resonance spectroscopy (MRS) and in vitro analysis of biopsies. Healthy subjects [4 young males, 28.2 +/- 6.8 (SD) yr, and 6 older subjects (5 males, 1 female), 66 +/- 6.0 yr] performed a maximal cycle ergometer test, and MRS measurements of the calf muscles and needle biopsies of the lateral gastrocnemius were performed. Biopsies were analyzed for fiber type and citrate synthase (CS) activity. MRS measurements of inorganic phosphate (Pi), phosphocreatine (PCr), ATP, and pH were made using a 1.8-T 78-cm clear-bore magnet-and-spectrometer system. Two or three 5-min bouts of plantar flexion were performed against variable resistance to deplete PCr levels to 50% of resting values (mean end pH 6.99). PCr values during recovery were fit to an exponential curve, and the rate constant (PCrrate) was calculated. PCrrate was used as an index of oxidative metabolism. Older subjects had lower peak O2 uptake (VO2 peak) values (19.2 +/- 5.6 vs. 49.5 +/- 8.1 ml O2.min-1 x kg-1), CS activities (16 +/- 2.8 vs. 25 +/- 2.6 mmol.kg wet wt-1 x min-1), and PCrrate values (25.3 +/- 8. vs. 37.5 +/- 5.3 mmol PCr.kg wet wt-1.min-1) than young subjects. PCrrate correlated with CS activity, and both PCrrate and CS activity correlated with VO2 peak (P < 0.05). No correlations were found between percent fiber type and PCrrate, CS activity, and VO2 peak. These results support studies that showed decreases in muscle metabolism with age in healthy humans and show a good correlation between in vivo and in vitro measurements of oxidative metabolism.

Overview

Integrity signals

Registry

Crossref · 10.1152/jappl.1993.75.2.813

Cites

27 of 27 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 28 references

Cited by

Sorted by citations

Related

See all

Metadata

Authors
McCully, K. K., Fielding, R. A., Evans, W. J., Leigh, J. S., & Posner, J. D.
Affiliations
Department of Medicine, Medical College of Pennsylvania, Philadelphia 19129.
ORCID
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
1993-08-01
Volume
75
Issue
2
Pages
813-819
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/jappl.1993.75.2.813
OpenAlex
W2273652180
Cited by
288
References
28
Citations by year
2026: 3 · 2025: 5 · 2024: 7 · 2023: 6 · 2022: 10 · 2021: 4 · 2020: 10 · 2019: 11 · 2018: 9 · 2017: 7 · 2016: 10 · 2015: 13 · 2014: 15 · 2013: 16 · 2012: 13
FWCI
11.16
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p01ag003934-14)
  • National Institute on Aging (AG 03934)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
  • Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
Keywords
muscle metabolism, aging, oxidative metabolism, citrate synthase activity, triceps surae, lateral gastrocnemius, inorganic phosphate, muscle fiber type, peak oxygen uptake, plantar flexion, magnetic resonance spectroscopy, human skeletal muscle
MeSH terms
Adenosine Triphosphate, Adult, Aged, Aging, Citrate (si)-Synthase, Exercise Test, Female, Humans, Leg, Male, Middle Aged, Muscles, Magnetic Resonance Spectroscopy, Oxidation-Reduction, Oxygen Consumption, Phosphates, Phosphocreatine, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review