Additive effect of contractions and insulin on GLUT-4 translocation into the sarcolemma
Abstract
The maximal effects of insulin and muscle contractions on glucose transport are additive. GLUT-4 is the major glucose transporter isoform expressed in skeletal muscle. Muscle contraction and insulin each induce translocation of GLUT-4 from intracellular sites into the plasma membrane. The purpose of this study was to test the hypothesis that the incremental effect of contractions and insulin on glucose transport is mediated by additivity of the maximal effects of these stimuli on GLUT-4 translocation into the sarcolemma. Anesthetized rats were given insulin by intravenous infusion to raise plasma insulin to 2,635 +/- 638 microU/ml. The gastrocnemius-plantaris-soleus group was stimulated to contract via the sciatic nerve by using a protocol that maximally activates glucose transport. After treatment with insulin, contractions, or insulin plus contractions or no treatment, the gastrocnemius-plantaris-soleus muscle group was dissected out and was subjected to subcellular fractionation to separate the plasma membrane and intracellular membrane fractions. Insulin induced a 70% increase and contractions induced a 113% increase in the GLUT-4 content of the plasma membrane fraction. The effects of insulin and contractions were additive, as evidenced by a 185% increase in the GLUT-4 content of the sarcolemmal fraction. This finding provides evidence that the incremental effect of maximally effective insulin and contractile stimuli on glucose transport is mediated by additivity of their effects on GLUT-4 translocation into the sarcolemma.
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Insulin is additive to contraction in stimulating glucose uptake.
“3) Insulin is additive to contraction in stimulating glucose uptake [109,110].”
From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [110]
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PAULO ALEXANDRE DE CARVALHO MORAES
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Duration of improved insulin sensitivity after high intensity exercise in young overweight men
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- Authors
- Gao, J., Ren, J., Gulve, E. A., & Holloszy, J. O.
- Affiliations
- Washington University in St. Louis (US)
- ORCID
- Gao, J.
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 1994-10-01
- Volume
- 77
- Issue
- 4
- Pages
- 1597-1601
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W1864159588
- Cited by
- 116
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 6 · 2015: 7 · 2014: 4 · 2013: 3 · 2012: 2
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG 00078)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK20579, DK-18986, R01DK018986)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- GLUT4, glucose transporter translocation, skeletal muscle glucose transport, insulin, muscle contraction, additive effects, sarcolemma, plasma membrane, subcellular fractionation
- MeSH terms
- Animals, Cell Fractionation, Cell Membrane, Electrophoresis, Polyacrylamide Gel, Glucose, Insulin, Intracellular Membranes, Male, Monosaccharide Transport Proteins, Muscle Contraction, Muscle Proteins, Sarcolemma, Blotting, Western, Rats, Wistar, Muscle, Skeletal, Glucose Transporter Type 4, Rats
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