Cited by 121Physical Activity and HealthMetabolism, Diabetes, and CancerCardiovascular and exercise physiology

Frequent interruptions of sedentary time modulates contraction- and insulin-stimulated glucose uptake pathways in muscle: Ancillary analysis from randomized clinical trials

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Bergouignan, Audrey, Latouche, Celine, Heywood, Sarah, Grace, Megan S., Reddy-Luthmoodoo, Medini, Natoli, Alaina K., Owen, Neville, Dunstan, David W. +1 more·2016·Scientific Reports

Abstract

Epidemiological studies have observed associations between frequent interruptions of sitting time with physical activity bouts and beneficial metabolic outcomes, even in individuals who regularly exercise. Frequent interruptions to prolonged sitting reduce postprandial plasma glucose. Here we studied potential skeletal muscle mechanisms accounting for this improved control of glycemia in overweight adults under conditions of one day uninterrupted sitting and sitting interrupted with light-intensity or moderate-intensity walking every 20-min (n = 8); and, after three days of either uninterrupted sitting or light-intensity walking interruptions (n = 5). Contraction- and insulin-mediated glucose uptake signaling pathways as well as changes in oxidative phosphorylation proteins were examined. We showed that 1) both interventions reduce postprandial glucose concentration, 2) acute interruptions to sitting over one day stimulate the contraction-mediated glucose uptake pathway, 3) both acute interruptions to sitting with moderate-intensity activity over one day and light-intensity activity over three days induce a transition to modulation of the insulin-signaling pathway, in association with increased capacity for glucose transport. Only the moderate-intensity interruptions resulted in greater capacity for glycogen synthesis and likely for ATP production. These observations contribute to a mechanistic explanation of improved postprandial glucose metabolism with regular interruptions to sitting time, a promising preventive strategy for metabolic diseases.

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Authors
Bergouignan, A., Latouche, C., Heywood, S., Grace, M. S., Reddy-Luthmoodoo, M., Natoli, A. K., Owen, N., Dunstan, D. W., & Kingwell, B. A.
Affiliations
Baker Heart and Diabetes Institute (AU)
Corresponding author
Bergouignan, Audrey
Venue
Scientific Reports
Published
2016-08-24
Volume
6
Issue
1
Pages
32044-32044
ISSN
2045-2322 (ISSN-L)
OpenAlex
W2512124094
Cited by
121
References
51
Citations by year
2026: 12 · 2025: 9 · 2024: 5 · 2023: 14 · 2022: 12 · 2021: 16 · 2020: 5 · 2019: 16 · 2018: 14 · 2017: 14 · 2016: 4
FWCI
12.87
Type
journal article
Language
en
Open access
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • Monash University Research Portal (Monash University) (publishedVersion, other-oa)
  • PubMed (publishedVersion)
  • Minerva Access (University of Melbourne) (submittedVersion, cc-by)
  • UWA Profiles and Research Repository (UWA) (submittedVersion, cc-by)
  • Deakin Research Online (Deakin University) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
  • Swinburne figshare (Swinburne University of Technology) (submittedVersion, cc-by)
  • Swinburne figshare (Swinburne University of Technology) (cc-by)
Funders
  • American Diabetes Association
  • Medical Research Council
  • National Health and Medical Research Council
Topics
  • Health Sciences › Medicine › Physiology › Physical Activity and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords
sedentary behavior, sitting interruptions, walking breaks, postprandial blood glucose, skeletal muscle glucose uptake, insulin signaling, oxidative phosphorylation, glycogen synthesis, glucose transport, glycemic control, overweight adults, metabolic disease prevention
MeSH terms
Acetyl-CoA Carboxylase, Blood Glucose, Glucose, Humans, Insulin, Middle Aged, Muscle Contraction, Oxidative Phosphorylation, Phosphorylation, Muscle, Skeletal, Postprandial Period, GTPase-Activating Proteins, Proto-Oncogene Proteins c-akt, Sedentary Behavior

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