Frequent interruptions of sedentary time modulates contraction- and insulin-stimulated glucose uptake pathways in muscle: Ancillary analysis from randomized clinical trials
Open accessAbstract
Epidemiological studies have observed associations between frequent interruptions of sitting time with physical activity bouts and beneficial metabolic outcomes, even in individuals who regularly exercise. Frequent interruptions to prolonged sitting reduce postprandial plasma glucose. Here we studied potential skeletal muscle mechanisms accounting for this improved control of glycemia in overweight adults under conditions of one day uninterrupted sitting and sitting interrupted with light-intensity or moderate-intensity walking every 20-min (n = 8); and, after three days of either uninterrupted sitting or light-intensity walking interruptions (n = 5). Contraction- and insulin-mediated glucose uptake signaling pathways as well as changes in oxidative phosphorylation proteins were examined. We showed that 1) both interventions reduce postprandial glucose concentration, 2) acute interruptions to sitting over one day stimulate the contraction-mediated glucose uptake pathway, 3) both acute interruptions to sitting with moderate-intensity activity over one day and light-intensity activity over three days induce a transition to modulation of the insulin-signaling pathway, in association with increased capacity for glucose transport. Only the moderate-intensity interruptions resulted in greater capacity for glycogen synthesis and likely for ATP production. These observations contribute to a mechanistic explanation of improved postprandial glucose metabolism with regular interruptions to sitting time, a promising preventive strategy for metabolic diseases.
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Sedentary behavior involves a lack of leg muscle contractions and restricted blood flow behind the knee when sitting, which causes reduced insulin sensitivity, vascular dysfunction, activation of low-grade inflammatory responses, and an imbalance in energy expenditure.
“It involves a lack of leg muscle contractions and restricted blood flow behind the knee when sitting, which causes reduced insulin sensitivity, vascular dysfunction, activation of low-grade inflammatory responses, and an imbalance in energy expenditure [3].”
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- Authors
- Bergouignan, A., Latouche, C., Heywood, S., Grace, M. S., Reddy-Luthmoodoo, M., Natoli, A. K., Owen, N., Dunstan, D. W., & Kingwell, B. A.
- Affiliations
- Baker Heart and Diabetes Institute (AU)
- Corresponding author
- Bergouignan, Audrey
- ORCID
- Venue
- Scientific Reports
- Published
- 2016-08-24
- Volume
- 6
- Issue
- 1
- Pages
- 32044-32044
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4995429
- OpenAlex
- W2512124094
- Cited by
- 121
- References
- 51
- Citations by year
- 2026: 12 · 2025: 9 · 2024: 5 · 2023: 14 · 2022: 12 · 2021: 16 · 2020: 5 · 2019: 16 · 2018: 14 · 2017: 14 · 2016: 4
- FWCI
- 12.87
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- Monash University Research Portal (Monash University) (publishedVersion, other-oa)
- PubMed (publishedVersion)
- Minerva Access (University of Melbourne) (submittedVersion, cc-by)
- UWA Profiles and Research Repository (UWA) (submittedVersion, cc-by)
- Deakin Research Online (Deakin University) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Swinburne figshare (Swinburne University of Technology) (submittedVersion, cc-by)
- Swinburne figshare (Swinburne University of Technology) (cc-by)
- Funders
- American Diabetes Association
- Medical Research Council
- National Health and Medical Research Council
- Topics
- Health Sciences › Medicine › Physiology › Physical Activity and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- sedentary behavior, sitting interruptions, walking breaks, postprandial blood glucose, skeletal muscle glucose uptake, insulin signaling, oxidative phosphorylation, glycogen synthesis, glucose transport, glycemic control, overweight adults, metabolic disease prevention
- MeSH terms
- Acetyl-CoA Carboxylase, Blood Glucose, Glucose, Humans, Insulin, Middle Aged, Muscle Contraction, Oxidative Phosphorylation, Phosphorylation, Muscle, Skeletal, Postprandial Period, GTPase-Activating Proteins, Proto-Oncogene Proteins c-akt, Sedentary Behavior
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