Enzyme-and gene-based approaches for developing methanogen-specific compounds to control ruminant methane emissions: a review

Henderson, Gemma; Cook, Gregory M.; Ronimus, Ron S.

doi:10.1071/an15757

Cited by 31Ruminant Nutrition and Digestive PhysiologyAnaerobic Digestion and Biogas ProductionAgriculture Sustainability and Environmental Impact

Enzyme-and gene-based approaches for developing methanogen-specific compounds to control ruminant methane emissions: a review

Henderson, Gemma, Cook, Gregory M., Ronimus, Ron S.·2018·Animal Production Science

DOI 10.1071/an15757

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Abstract

Methane emissions from ruminants are of worldwide concern due to their potential to adversely affect climate patterns. Methane emissions can be mitigated in several ways, including dietary manipulation, the use of alternative hydrogen sinks, and by the direct inhibition of methanogens. In the present review, we summarise and emphasise studies where defined chemically synthesised compounds have been used to mitigate ruminant methane emissions by direct targeting of methanogens and discuss the future potential of such inhibitors. We also discuss experiments, where methanogen-specific enzymes and pure cultures of methanobacterial species have been used to aid development of inhibitors. Application of certain compounds can result in dramatic reductions of methane emissions from ruminant livestock, demonstrating ‘proof of principle’ of chemical inhibitors of methanogenesis. More recently, genome sequencing of rumen methanogens has enabled an in-depth analysis of the enzymatic pathways required for methane formation. Chemogenomic methods, similar to those used in the fight against cancer and infectious diseases, can now be used to specifically target a pathway or enzyme in rumen methanogens. However, few rumen methanogen enzymes have been structurally or biochemically characterised. Any compound, whether natural or man-made, that is used as a mitigation strategy will need to be non-toxic to the host animal (and humans), cost-effective, environmentally friendly, and not accumulate in host tissues or milk products. Chemically synthesised inhibitors offer potentially significant advantages, including high levels of sustained inhibition, the ability to be easily and rapidly produced for global markets, and have the potential to be incorporated into slow-release vehicles for grazing animals.

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Enhanced expression of some methanogenic genes may lead to enhanced methanogenesis that ultimately compensates for a decrease in the overall number of methanogens.
“This is mainly because the enhanced expression of some methanogenic genes may lead to enhanced methanogenesis that ultimately compensated a decrease in the overall number of methanogens (156).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [156]

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Enzyme- and gene-based approaches for developing methanogen-specific compounds to control ruminant methane emissions
Henderson, G., (777541), G. C., & Ronimus, R. S. (2017) · Figshare
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High-throughput screening for the discovery of novel inhibitors to mitigate ruminant methane emissions
Schofield, L. R., (414553), Y.-L. Z., Sang, C., Atua, R., Molano, S., Beattie, A. K., Dey, D., (15395435), G. C., (15395429), J. C., (12289307), Y. K., et al. (2016) · Figshare
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Authors: Henderson, G., Cook, G. M., & Ronimus, R. S.
Affiliations: AgResearch (NZ)
University of Otago (NZ)
Corresponding author: Cook, Gregory M.
Ronimus, Ron S.
ORCID: Henderson, Gemma
Cook, Gregory M.
Ronimus, Ron S.
Venue: Animal Production Science
Publisher: CSIRO Publishing
Published: 2016-04-05
Volume: 58
Issue: 6
Pages: 1017-1026
ISSN: 1836-0939 (ISSN-L)
1836-5787
DOI: 10.1071/an15757
OpenAlex: W2224571207
Cited by: 31
References: 100
Citations by year: 2026: 1 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 5 · 2021: 7 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 3
FWCI: 4.79
Type: journal article
Language: en
Open access: closed
Licence: doi.org/10.1071/journalslicense (vor, 3317 d)
Locations: Animal Production Science (publishedVersion)
Figshare (submittedVersion)
Funders: New Zealand Government
New Zealand Agricultural Greenhouse Gas Research Centre
Topics: Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Physical Sciences › Environmental Science › Ecology › Agriculture Sustainability and Environmental Impact
Keywords: ruminant methane emissions, rumen methanogens, chemogenomics, hydrogen sink, dietary intervention, methane mitigation, Methanobacterium

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Enzyme-and gene-based approaches for developing methanogen-specific compounds to control ruminant methane emissions: a review

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