The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
tRNA-Derived Fragments Target the Ribosome and Function as Regulatory Non-Coding RNA in Haloferax volcanii
Open accessAbstract
Nonprotein coding RNA (ncRNA) molecules have been recognized recently as major contributors to regulatory networks in controlling gene expression in a highly efficient manner. These RNAs either originate from their individual transcription units or are processing products from longer precursor RNAs. For example, tRNA-derived fragments (tRFs) have been identified in all domains of life and represent a growing, yet functionally poorly understood, class of ncRNA candidates. Here we present evidence that tRFs from the halophilic archaeon Haloferax volcanii directly bind to ribosomes. In the presented genomic screen of the ribosome-associated RNome, a 26-residue-long fragment originating from the 5′ part of valine tRNA was by far the most abundant tRF. The Val-tRF is processed in a stress-dependent manner and was found to primarily target the small ribosomal subunit in vitro and in vivo. As a consequence of ribosome binding, Val-tRF reduces protein synthesis by interfering with peptidyl transferase activity. Therefore this tRF functions as ribosome-bound small ncRNA capable of regulating gene expression in H. volcanii under environmental stress conditions probably by fine tuning the rate of protein production.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Mattick, J. S., & Makunin, I. V. (2006) · Human Molecular Genetics · doi:10.1093/hmg/ddl046 · Cited by 2,453
A novel class of small RNAs: tRNA-derived RNA fragments (tRFs)
Lee, Y. S., Shibata, Y., Malhotra, A., & Dutta, A. (2009) · Genes & Development · doi:10.1101/gad.1837609 · Cited by 1,173
Molecular mechanisms of translational control
Gebauer, F., & Hentze, M. W. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1488 · Cited by 991
Angiogenin-Induced tRNA Fragments Inhibit Translation Initiation
Ivanov, P., Emara, M. M., Villen, J., Gygi, S. P., & Anderson, P. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.06.022 · Cited by 985
Angiogenin cleaves tRNA and promotes stress-induced translational repression
Yamasaki, S., Ivanov, P., Hu, G.-F., & Anderson, P. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200811106 · Cited by 936
A Human snoRNA with MicroRNA-Like Functions
Ender, C., Krek, A., Friedländer, M. R., Beitzinger, M., Weinmann, L., Chen, W., Pfeffer, S., Rajewsky, N., & Meister, G. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.10.017 · Cited by 844
Human tRNA-derived small RNAs in the global regulation of RNA silencing
Haussecker, D., Huang, Y., Lau, A., Parameswaran, P., Fire, A. Z., & Kay, M. A. (2010) · RNA · doi:10.1261/rna.2000810 · Cited by 713
Cole, C., Sobala, A., Lu, C., Thatcher, S. R., Bowman, A., Brown, J. W. S., Green, P. J., Barton, G. J., & Hutvagner, G. (2009) · RNA · doi:10.1261/rna.1738409 · Cited by 645
The Eukaryotic Genome as an RNA Machine
Amaral, P. P., Dinger, M. E., Mercer, T. R., & Mattick, J. S. (2008) · Science · doi:10.1126/science.1155472 · Cited by 619
Stress induces tRNA cleavage by angiogenin in mammalian cells
Fu, H., Feng, J., Liu, Q., Sun, F., Tie, Y., Zhu, J., Xing, R., Sun, Z., & Zheng, X. (2009) · FEBS Letters · doi:10.1016/j.febslet.2008.12.043 · Cited by 571
Cited by
Aberrant methylation of t RNA s links cellular stress to neuro‐developmental disorders
Blanco, S., Dietmann, S., Flores, J. V., Hussain, S., Kutter, C., Humphreys, P., Lukk, M., Lombard, P., Treps, L., Popis, M., et al. (2014) · The EMBO Journal · doi:10.15252/embj.201489282 · Cited by 620
Biogenesis and Function of Transfer RNA-Related Fragments (tRFs)
Kumar, P., Kuscu, C., & Dutta, A. (2016) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2016.05.004 · Cited by 511
Stem cell function and stress response are controlled by protein synthesis
Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortés-Garrido, R., Gkatza, N., et al. (2016) · Nature · doi:10.1038/nature18282 · Cited by 466
tRNA fragments in human health and disease
Anderson, P., & Ivanov, P. (2014) · FEBS Letters · doi:10.1016/j.febslet.2014.09.001 · Cited by 386
The presence, role and clinical use of spermatozoal RNAs
Jodar, M., Selvaraju, S., Sendler, E., Diamond, M. P., Krawetz, S. A., & for the Reproductive Medicine Network (2013) · Human Reproduction Update · doi:10.1093/humupd/dmt031 · Cited by 368
Metadata
- Authors
- Gebetsberger, J., Zywicki, M., Künzi, A., & Polacek, N.
- Venue
- Archaea
- Publisher
- Wiley
- Published
- 2012
- Volume
- 2012
- Pages
- 1-11
- ISSN
- 1472-3646 (ISSN-L)
- 1472-3654
- OpenAlex
- W2097331197
- Cited by
- 269
- References
- 34
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: tRNA-Derived Fragments Target the Ribosome and Function as Regulatory Non-Coding RNA in Haloferax volcanii. https://app.cochat.ai/papers/10.1155/2012/260909
- The paper
- Gebetsberger, J., Zywicki, M., Künzi, A., & Polacek, N. (2012). tRNA-Derived Fragments Target the Ribosome and Function as Regulatory Non-Coding RNA in Haloferax volcanii. Archaea. https://doi.org/10.1155/2012/260909
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
