In Vitro and In Vivo Anti‐AChE and Antioxidative Effects of Schisandra chinensis Extract: A Potential Candidate for Alzheimer’s Disease
Open accessAbstract
Acetylcholinesterase (AChE) inhibition and antioxidants are two common strategies for the treatment in the early stage of Alzheimer’s Disease (AD). In this study, extracts from nine traditional Chinese medical (TCM) herbs were tested for anti‐AChE activity by Ellman’s microplate assay and cytotoxicity by CCK‐8. Based on its excellent AChE inhibition effect and its lowest cytotoxicity, Schisandra chinensis (SC) extract was selected to do the mechanism research. SC extract protected pheochromocytoma (PC12) cells against H2O2‐induced toxicity by improving the cell survival rate in a dose‐dependent manner. And it also showed significant free radical (DPPH) scavenging activities, ferric reducing antioxidant power (FRAP), and 2,2′‐Azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) radical scavenging. To confirm these results, the scopolamine‐induced mice models were utilized in this study. Compared with the positive drug (piracetam), SC could also exhibit similar effects to alleviate the mice’s cognitive deficits. Moreover, in the mice brain samples, the AChE activity and malondialdehyde (MDA) levels of SC‐treatment group both showed a reverse as compared to model group. Taken together, these results all suggested that SC extract may be a potential therapeutic candidate for AD.
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Extracts from Ocimum basilicum, Lavandula stoechas, Schisandra chinensis, and Ginkgo biloba had neuroprotective effects with significant reductions in AChE activity (p<0.001) and improvements in memory and oxidative stress markers.
“Further investigations into extracts from Ocimum basilicum, Lavandula stoechas, Schisandra chinensis, and Ginkgo biloba have highlighted their neuroprotective effects, with significant reductions in AChE activity (p < 0.001) and improvements in memory and oxidative stress markers [49,50,51,52,53].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [51]
Results §§3.3 and 3.6–3.7 (page not available) report neuroprotection, improved water-maze performance, and reduced AChE activity and brain MDA after Schisandra chinensis extract treatment. The paper does not report p < 0.001 for the AChE reduction; it reports p < 0.01 for MDA, while SOD and FRAP did not improve. It studies Schisandra chinensis, not the other three named plants. (LLM, conf=0.89)
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- Authors
- Song, X., Wang, T., Guo, L., Jin, Y., Wang, J., Yin, G., Jiang, K., Wang, L., Huang, H., & Zeng, L.
- Affiliations
- Shenzhen Institute for Drug Control (CN)
- Corresponding author
- Wang, Tiejie
- Venue
- Evidence-based Complementary and Alternative Medicine
- Publisher
- Wiley
- Published
- 2020-01
- Volume
- 2020
- Issue
- 1
- Pages
- 2804849-2804849
- ISSN
- 1741-427X (ISSN-L)
- 1741-4288
- PMC
- PMC7053447
- OpenAlex
- W3007235112
- Cited by
- 8
- References
- 36
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2022: 3 · 2021: 1
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Evidence-based Complementary and Alternative Medicine (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Science and Technology Planning Project of Shenzen Municipality (JCYJ20170817141236095)
- Topics
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Keywords
- Schisandra chinensis, Alzheimer's disease, acetylcholinesterase inhibition, antioxidant activity, Chinese herbal medicine, PC12 cells, hydrogen peroxide stress, scopolamine-induced cognitive deficits, malondialdehyde, DPPH radical scavenging, piracetam, neuroprotection
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