The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Serum microRNA Profiles Serve as Novel Biomarkers for HBV Infection and Diagnosis of HBV-Positive Hepatocarcinoma
Open accessAbstract
Diagnosis of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), particularly HCC independent of cirrhosis etiology, presents a great challenge because of a lack of biomarkers. Here we test the hypothesis that expression profiles of microRNAs (miRNAs) in serum can serve as biomarkers for diagnosis of HBV infection and HBV-positive HCC. We recruited 513 subjects (210 controls and 135 HBV-, 48 hepatitis C virus (HCV)-, and 120 HCC-affected individuals) and employed a strategy of initial screening by Solexa sequencing followed by validation with TaqMan probe-based quantitative reverse transcription-PCR assay. First, because of a close link between chronic hepatitis B and HCC, we compared miRNA expression profiles in HBV serum with that in control serum and successfully obtained 13 miRNAs that were differentially expressed in HBV serum. This 13-miRNA–based biomarker accurately discriminated not only HBV cases from controls and HCV cases, but also HBV-positive HCC cases from control and HBV cases. Second, we directly compared miRNA expressions in HCC serum with those in controls and identified 6 miRNAs that were significantly upregulated in HCC samples. Interestingly, 2 of these miRNAs, miR-375 and miR-92a, were also identified by our first approach as HBV specific. When we employed 3 of these miRNAs (miR-25, miR-375, and let-7f) as biomarkers, we could clearly separate HCC cases from controls, and miR-375 alone had an ROC of 0.96 (specificity: 96%; sensitivity: 100%) in HCC prediction. In conclusion, our study demonstrates for the first time that serum miRNA profiles can serve as novel and noninvasive biomarkers for HBV infection and HBV-positive HCC diagnosis. Cancer Res; 70(23); 9798–807. ©2010 AACR.
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1 retracted
Datta, J., Kutay, H., Nasser, M. W., Nuovo, G. J., Wang, B., Majumder, S., Liu, C.-G., Volinia, S., Croce, C. M., Schmittgen, T. D., et al. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-6655 · Cited by 438
RetractedJemal, A., Bray, F., Center, M. M., Ferlay, J., Ward, E., & Forman, D. (2011) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.20107 · Cited by 54,679
Global Cancer Statistics, 2002
Parkin, D. M., Bray, F., Ferlay, J., & Pisani, P. (2005) · CA: A Cancer Journal for Clinicians · doi:10.3322/canjclin.55.2.74 · Cited by 18,196
The functions of animal microRNAs
Ambros, V. (2004) · Nature · doi:10.1038/nature02871 · Cited by 10,728
Circulating microRNAs as stable blood-based markers for cancer detection
Mitchell, P. S., Parkin, R. K., Kroh, E. M., Fritz, B. R., Wyman, S. K., Pogosova-Agadjanyan, E. L., Peterson, A., Noteboom, J., O'Briant, K. C., Allen, A., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0804549105 · Cited by 7,969
Management of Hepatocellular Carcinoma *
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Self-citationMicroRNA-21 Regulates Expression of the PTEN Tumor Suppressor Gene in Human Hepatocellular Cancer
Meng, F., Henson, R., Wehbe–Janek, H., Ghoshal, K., Jacob, S. T., & Patel, T. (2007) · Gastroenterology · doi:10.1053/j.gastro.2007.05.022 · Cited by 2,773
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Huang, H.-Y., Lin, Y.-C.-D., Li, J., Huang, K.-Y., Shrestha, S., Hong, H.-C., Tang, Y., Chen, Y.-G., Jin, C.-N., Yu, Y., et al. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz896 · Cited by 1,259
Self-citationZhang, L., Hou, D., Chen, X., Li, D., Zhu, L., Zhang, Y., Li, J., Bian, Z., Liang, X., Cai, X., et al. (2012) · Cell Research · doi:10.1038/cr.2011.158 · Cited by 1,216
Self-citationHsu, S.-D., Tseng, Y.-T., Shrestha, S., Lin, Y.-L., Khaleel, A., Chou, C.-H., Chu, C.-F., Huang, H.-Y., Lin, C.-M., Ho, S.-Y., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1266 · Cited by 1,041
Huang, H.-Y., Lin, Y.-C.-D., Cui, S., Huang, Y., Tang, Y., Xu, J., Bao, J., Li, Y., Wen, J., Zuo, H., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkab1079 · Cited by 983
Self-citationCirculating microRNAs as novel biomarkers for diabetes mellitus
Guay, C., & Regazzi, R. (2013) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2013.86 · Cited by 563
Metadata
- Authors
- Li, L.-M., Hu, Z.-B., Zhou, Z.-X., Chen, X., Liu, F.-Y., Zhang, J.-F., Shen, H.-B., Zhang, C.-Y., & Zen, K.
- Venue
- Cancer Research
- Published
- 2010-12-01
- Volume
- 70
- Issue
- 23
- Pages
- 9798-9807
- ISSN
- 0008-5472 (ISSN-L)
- 1538-7445
- OpenAlex
- W2162769953
- Cited by
- 451
- References
- 46
- FWCI
- 10.39
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Serum microRNA Profiles Serve as Novel Biomarkers for HBV Infection and Diagnosis of HBV-Positive Hepatocarcinoma. https://app.cochat.ai/papers/10.1158/0008-5472.can-10-1001
- The paper
- Li, L.-M., Hu, Z.-B., Zhou, Z.-X., Chen, X., Liu, F.-Y., Zhang, J.-F., Shen, H.-B., Zhang, C.-Y., & Zen, K. (2010). Serum microRNA Profiles Serve as Novel Biomarkers for HBV Infection and Diagnosis of HBV-Positive Hepatocarcinoma. Cancer Research. https://doi.org/10.1158/0008-5472.can-10-1001
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