Evidence that an endothelial cytosolic protein binds to the 3′-untranslated region of endothelial nitric oxide synthase mRNA
Abstract
Changes in the endothelial nitric oxide synthase (eNOS) expression could be involved in the endothelium-dependent vasorelaxing dysfunction associated with cardiovascular diseases. We have recently demonstrated the existence of endothelial cytosolic proteins that bind to the 3'-untranslated region (3'-UTR) of eNOS mRNA and could be involved in eNOS mRNA stabilization. In the present work, we have characterized the cytosolic proteins that bind to 3'-UTR eNOS mRNA. An endothelial cytosolic protein (MW 60-kD) specifically bound to 3'-UTR eNOS mRNA as determined by a cross-linking assay followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The endothelial cytosolic protein recognized a cytidine (C)-rich region within 3'-UTR eNOS mRNA. Furthermore, tumor necrosis factor-alpha (TNF-alpha) increased the level of the 60-kD endothelial cytosolic protein. In addition, TNF-alpha reduced eNOS mRNA levels and this was prevented by coincubation with cycloheximide. Cycloheximide also prevented the binding activity of the endothelial cytosolic protein to 3'-UTR eNOS mRNA. In summary, these data suggest that a 60-kD endothelial cytosolic protein binds to 3'-UTR eNOS mRNA. TNF-alpha increased the 60-kD protein levels. Cycloheximide prevented the binding activity of the cytosolic protein to 3'-UTR eNOS mRNA related to TNF-alpha; this effect was associated with greater eNOS mRNA levels. Further specific studies are needed to determine the involvement of this 60-kD endothelial cytosolic protein in the regulation of eNOS mRNA stabilization and in the endothelial dysfunction associated with cardiovascular diseases.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
TNFα downregulates NOS3 expression and abundance by decreasing the stability of NOS3 mRNA, effectively shortening its half-life.
“was shown to downregulate NOS3 expression and abundance [123, 131-134] by decreasing the stability of NOS3 mRNA [135, 136], effectively shortening its half-life [137].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [136]
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
The L-Arginine-Nitric Oxide Pathway
Epstein, F. H., Moncada, S., & Higgs, A. (1993) · New England Journal of Medicine · doi:10.1056/nejm199312303292706 · Cited by 6,678 · see citing works
Nitric oxide: an endogenous modulator of leukocyte adhesion.
Kubes, P., Suzuki, M., & Granger, D. N. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.11.4651 · Cited by 3,052 · see citing works
mRNA stability in mammalian cells
Ross, J. (1995) · Microbiological Reviews · doi:10.1128/mr.59.3.423-450.1995 · Cited by 1,324 · see citing works
Sessa, W. C., Pritchard, K., Seyedi, N., Wang, J., & Hintze, T. H. (1994) · Circulation Research · doi:10.1161/01.res.74.2.349 · Cited by 934 · see citing works
Yoshizumi, M., Perrella, M. A., Burnett, J. C., & Lee, M. E. (1993) · Circulation Research · doi:10.1161/01.res.73.1.205 · Cited by 771 · see citing works
Müllner, E. W., & Kühn, L. C. (1988) · Cell · doi:10.1016/0092-8674(88)90098-0 · Cited by 554 · see citing works
Cytoplasmic regulation of mRNA function: The importance of the 3′ untranslated region
Jackson, R. J. (1993) · Cell · doi:10.1016/0092-8674(93)90290-7 · Cited by 388 · see citing works
Vakalopoulou, E., Schaack, J. B., & Shenk, T. E. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.6.3355 · Cited by 266 · see citing works
Cras, T. D. L., Xue, C., Rengasamy, A., & Johns, R. A. (1996) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.1996.270.1.l164 · Cited by 254 · see citing works
Effect of endothelin-1 on neutrophil adhesion to endothelial cells and perfused heart.
Farré, A. L., Riesco, A., Espinosa, G., Digiuni, E., Cernadas, M. R., Álvarez, V., Montón, M., Rivas, F., Gallego, M. J., & Egido, J. (1993) · Circulation · doi:10.1161/01.cir.88.3.1166 · Cited by 192 · see citing works
Self-citationWang, X., Kiledjian, M., Weiss, I. M., & Liebhaber, S. A. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.3.1769 · Cited by 192 · see citing works
Effects of Aspirin on Platelet-Neutrophil Interactions
López-Farré, A. J., Caramelo, C. A., Esteban, A., Alberola, M. L., Millás, I., Montón, M., & Casado, S. (1995) · Circulation · doi:10.1161/01.cir.91.7.2080 · Cited by 183 · see citing works
Self-citationAlonso, J., Sánchez de Miguel, L., Montón, M., Casado, S., & López-Farré, A. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.10.5719 · Cited by 146 · see citing works
Port, J. D., Huang, L. Y., & Malbon, C. C. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35952-0 · Cited by 118 · see citing works
Zaidi, S. H. E., & Malter, J. S. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.29.17292 · Cited by 110 · see citing works
Dodson, R. E., & Shapiro, D. J. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.5.3130-3138.1994 · Cited by 84 · see citing works
Mohamed, F., Monge, J. C., Gordon, A. E., Černáček, P., Blais, D., & Stewart, D. J. (1995) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.15.1.52 · Cited by 80 · see citing works
Regulation of mRNA stability and its relevance to disease.
Carter, B. Z., & Malter, J. S. (1991) · PubMed · Cited by 66 · see citing works
Chen, F. Y., Amara, F., & Wright, J. R. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06075.x · Cited by 64 · see citing works
Bickel, M., Iwai, Y., Pluznik, D. H., & Cohen, R. B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.21.10001 · Cited by 52 · see citing works
Amara, F., Chen, F. Y., & Wright, J. A. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.20.4803 · Cited by 51 · see citing works
Endothelial Cells Inhibit NO Generation by Vascular Smooth Muscle Cells
López-Farré, A. J., Mosquera, J. R., de Miguel, L. S., Millás, I., de Frutos, T., Montón, M., Sierra, M. P., Riesco, A., & Casado, S. (1996) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.16.10.1263 · Cited by 27 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Regulation of obesity and insulin resistance by nitric oxide
Sansbury, B. E., & Hill, B. G. (2014) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.05.016 · Cited by 274 · see citing works
“resistance [130], was shown to downregulate NOS3 expression and abundance [123,131–134] by decreasing the stability of NOS3 mRNA [135,136], effectively shortening its half-life [137].”
BackgroundTNF-α induces a decrease in eNOS promoter activity
Neumann, P., Gertzberg, N., & Johnson, A. (2004) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00378.2002 · Cited by 118 · see citing works
- Authors
- Sánchez de Miguel, L., Alonso, J., González-Fernández, F., de la Osada, J., Montón, M., Rodríguez-Feo, J. A., Guerra, J. I., Arriero, M. M., Rico, L., Casado, S., & López-Farré, A.
- Affiliations
- Hospital Universitario Fundación Jiménez Díaz (ES)
- Venue
- Journal of Vascular Research
- Publisher
- S. Karger AG
- Published
- 1999
- Volume
- 36
- Issue
- 3
- Pages
- 201-208
- ISSN
- 1018-1172 (ISSN-L)
- 1423-0135
- OpenAlex
- W2047624342
- Cited by
- 32
- References
- 24
- Citations by year
- 2019: 1 · 2016: 1 · 2015: 2 · 2014: 3
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Vascular Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- endothelial nitric oxide synthase, 3' untranslated region, mRNA stabilization, TNF-α, cycloheximide, endothelial dysfunction, cardiovascular disease, vasorelaxation
- MeSH terms
- Animals, Cattle, Cells, Cultured, Cycloheximide, Cytosol, Endothelium, Vascular, Proteins, RNA, Messenger, Tumor Necrosis Factor-alpha, Nitric Oxide Synthase, 3' Untranslated Regions, Nitric Oxide Synthase Type III
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
