Cited by 192RNA Research and SplicingRNA and protein synthesis mechanismsHemoglobinopathies and Related Disorders

Detection and Characterization of a 3′ Untranslated Region Ribonucleoprotein Complex Associated with Human α-Globin mRNA Stability

Open access
Wang, Xiaoming, Kiledjian, Megerditch, Weiss, Ingrid M., Liebhaber, Stephen A.·1995·Molecular and Cellular Biology

Abstract

The highly stable nature of globin mRNA is of central importance to erythroid cell differentiation. We have previously identified cytidine-rich (C-rich) segments in the human alpha-globin mRNA 3' untranslated region (alpha-3'UTR) which are critical in the maintenance of mRNA stability in transfected erythroid cells. In the present studies, we have detected trans-acting factors which interact with these cis elements to mediate this stabilizing function. A sequence-specific ribonucleoprotein (RNP) complex is assembled after incubation of the alpha-3'UTR with a variety of cytosolic extracts. This so-called alpha-complex is sequence specific and is not formed on the 3'UTR of either beta-globin or growth hormone mRNAs. Furthermore, base substitutions within the C-rich stretches which destabilize alpha-globin mRNA in vivo result in a parallel disruption of the alpha-complex in vitro. Competition studies with a series of homoribopolymers reveals a striking sensitivity of alpha-complex formation to poly(C), suggesting the presence of a poly(C)-binding activity within the alpha-complex. Three predominant proteins are isolated by alpha-3'UTR affinity chromatography. One of these binds directly to poly(C). This cytosolic poly(C)-binding protein is distinct from previously described nuclear poly(C)-binding heterogeneous nuclear RNPs and is necessary but not sufficient for alpha-complex formation. These data suggest that a messenger RNP complex formed by interaction of defined segments within the alpha-3'UTR with a limited number of cytosolic proteins, including a potentially novel poly(C)-binding protein, is of functional importance in establishing high-level stability of alpha-globin mRNA.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/mcb.15.3.1769

Cites

64 of 64 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 11

  1. 10.1128/mcb.14.6.4076-4086.1994 · DOI
  2. 10.1128/mcb.11.6.3288-3295.1991 · DOI
  3. 10.1128/mcb.11.5.2460-2466.1991 · DOI
  4. 10.1128/mcb.12.12.5748-5757.1992 · DOI
  5. 10.1128/mcb.12.12.5288-5300.1992 · DOI
  6. 10.1128/mcb.12.1.164-171.1992 · DOI
  7. 10.1128/mcb.8.5.2237-2241.1988 · DOI
  8. 10.1128/mcb.14.12.8123-8132.1994 · DOI
  9. 10.1128/mcb.13.8.5034-5042.1993 · DOI
  10. 10.1128/mcb.12.7.2931-2940.1992 · DOI
  11. 10.1128/mcb.13.12.7652-7665.1993 · DOI

Cited by

Sorted by citations

Authors
Wang, X., Kiledjian, M., Weiss, I. M., & Liebhaber, S. A.
Affiliations
Howard Hughes Medical Institute (US)
Venue
Molecular and Cellular Biology
Published
1995-03-01
Volume
15
Issue
3
Pages
1769-1777
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2114130204
Cited by
192
References
64
Citations by year
2025: 3 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 3 · 2014: 2 · 2013: 6 · 2012: 4
FWCI
4.77
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (P60HL038632)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Health Sciences › Medicine › Genetics › Hemoglobinopathies and Related Disorders
Keywords
alpha-globin mRNA, mRNA stability, 3' untranslated region, ribonucleoprotein complexes, trans-acting factors, poly(C), erythroid differentiation, mRNA-binding proteins, globin mRNA
MeSH terms
Animals, Base Sequence, Cell Line, Cell Nucleus, Chromatography, Affinity, Leukemia, Erythroblastic, Acute, Fibroblasts, Globins, Humans, Molecular Sequence Data, Poly C, Ribonucleoproteins, RNA, Messenger, Growth Hormone, Templates, Genetic, Transfection, Protein Biosynthesis, Oligonucleotide Probes, Polymerase Chain Reaction, Mutagenesis, Site-Directed, RNA-Binding Proteins, Point Mutation, DNA Primers, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review