Tumor necrosis factor downregulates an endothelial nitric oxide synthase mRNA by shortening its half-life
Abstract
Nitric oxide (NO), which accounts for the biological properties of endothelium-derived relaxing factor, is generated by NO synthase (NOS). The vascular endothelium contains two types of NOS: one is constitutively expressed (cNOS), and the other is inducible. Endothelium-mediated vasorelaxation is impaired in atherosclerotic vessels. To determine whether tumor necrosis factor (TNF)-alpha, which is commonly found in atherosclerotic lesions, has an effect on NOS message, we measured cNOS mRNA levels in TNF-treated human umbilical vein endothelial cells (HUVECs) by RNA blot analysis with a cNOS cDNA probe. TNF-alpha markedly reduced cNOS mRNA levels in HUVECs in a dose- and time-dependent manner. In response to 3 ng/mL TNF-alpha, cNOS mRNA levels began to decrease at 4 hours and diminished to only 5% of control levels at 24 hours. As little as 0.1 ng/mL TNF-alpha reduced cNOS mRNA levels by 50%. This reduction in cNOS message in response to TNF-alpha depended on protein synthesis as it was blocked by cycloheximide. In nuclear runoff experiments, TNF-alpha did not change the rate of cNOS gene transcription. cNOS mRNA is very stable under basal conditions, with a half-life of 48 hours; however, treatment with TNF-alpha shortened this half-life to 3 hours. TNF-alpha thus appears to decrease cNOS mRNA levels by increasing the rate of mRNA degradation. TNF-induced reductions in cNOS mRNA levels may have an important effect on impaired endothelium-mediated vasorelaxation in atherosclerosis.
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TNFα downregulates NOS3 expression and abundance by decreasing the stability of NOS3 mRNA, effectively shortening its half-life.
“was shown to downregulate NOS3 expression and abundance [123, 131-134] by decreasing the stability of NOS3 mRNA [135, 136], effectively shortening its half-life [137].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [137]
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“TNFα induces VEGF and its vasculotoxic effect has been attributed to prothrombotic action [57] reduction in cerebral blood flow [58] and production of nitric oxide and free radicals [59].”
BackgroundRegulation of endothelial-type NO synthase expression in pathophysiology and in response to drugs
Li, H., Wallerath, T., Münzel, T. F., & Förstermann, U. (2002) · Nitric Oxide · doi:10.1016/s1089-8603(02)00111-8 · Cited by 205 · see citing works
Coulet, F., Nadaud, S., Agrapart, M., & Soubrier, F. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305420200 · Cited by 203 · see citing works
Inactivation of catecholamines by superoxide gives new insights on the pathogenesis of septic shock
Macarthur, H., Westfall, T. C., Riley, D. P., Misko, T. P., & Salvemini, D. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.17.9753 · Cited by 203 · see citing works
Maas, R., Schwedhelm, E., Albsmeier, J., & Böger, R. H. (2002) · Vascular Medicine · doi:10.1191/1358863x02vm429ra · Cited by 199 · see citing works
Rössig, L., Li, H., Fißlthaler, B., Urbich, C., Fleming, I., Förstermann, U., Zeiher, A. M., & Dimmeler, S. (2002) · Circulation Research · doi:10.1161/01.res.0000037983.07158.b1 · Cited by 197 · see citing works
T L ymphocyte –E ndothelial C ell I nteractions
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Cardiac troponin I as diagnostic and prognostic marker in severe heart failure
La Vecchia, L., Mezzena, G., Zanolla, L., Paccanaro, M., Varotto, L., Bonanno, C., & Ometto, R. (2000) · The Journal of Heart and Lung Transplantation · doi:10.1016/s1053-2498(00)00120-0 · Cited by 191 · see citing works
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Molecular control of nitric oxide synthases in the cardiovascular system
Papapetropoulos, A. (1999) · Cardiovascular Research · doi:10.1016/s0008-6363(99)00161-3 · Cited by 186 · see citing works
Nitric Oxide Synthases: Gene Structure and Regulation
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Endothelial Dysfunction Driven by Hypoxia—The Influence of Oxygen Deficiency on NO Bioavailability
Janaszak‐Jasiecka, A., Siekierzycka, A., Płoska, A., Dobrucki, I. T., & Kalinowski, L. (2021) · Biomolecules · doi:10.3390/biom11070982 · Cited by 189 · see citing works
“Conversely, ECs activation was shown to contribute to endothelial dysfunction by inhibiting eNOS expression via decreasing eNOS promoter activity, as well as its mRNA half-life due to miR-155 induction [101,181,182].”
Background
- Authors
- Yoshizumi, M., Perrella, M. A., Burnett, J. C., & Lee, M. E.
- Affiliations
- Harvard University (US)
- Venue
- Circulation Research
- Published
- 1993-07
- Volume
- 73
- Issue
- 1
- Pages
- 205-209
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2145570729
- Cited by
- 771
- References
- 0
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 9 · 2023: 10 · 2022: 13 · 2021: 15 · 2020: 16 · 2019: 12 · 2018: 16 · 2017: 14 · 2016: 11 · 2015: 17 · 2014: 35 · 2013: 19 · 2012: 18
- FWCI
- 21.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Circulation Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- TNF-α, endothelial nitric oxide synthase, constitutive nitric oxide synthase, mRNA stability, mRNA degradation, human umbilical vein endothelial cells, atherosclerosis, cycloheximide, gene transcription
- MeSH terms
- Amino Acid Oxidoreductases, Base Sequence, Endothelium, Vascular, Half-Life, Humans, Molecular Sequence Data, RNA, Messenger, Transcription, Genetic, Tumor Necrosis Factor-alpha, Umbilical Veins, Down-Regulation, Polymerase Chain Reaction, Nitric Oxide Synthase
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