A stem-loop in the 3′ untranslated region mediates iron-dependent regulation of transferrin receptor mRNA stability in the cytoplasm
Open accessAbstract
Expression of the human transferrin receptor (hTR) and its mRNA is strongly induced by iron deprivation. By measuring transcription elongation rates, levels of hTR-specific nuclear RNA, and mRNA half-lives, we found this regulation to occur posttranscriptionally in the cytoplasm. Analysis of hTR cDNA mutants with deletions in the 3' untranslated region revealed the existence of two distinct domains, both of which are essential for regulation in mouse L cells. The regulated phenotype correlates with the presence of a stem-loop structure predicted by a computer algorithm. Expression of point and deletion mutants affecting the stem-loop confirmed the requirement for this secondary structure in regulation. The 3' untranslated region of hTR cDNA was sufficient to confer iron-dependent regulation on another gene.
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Upregulation of hepcidin during inflammation occurs, at least in part, through the action of IL-6.
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From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [214]
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From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [214]
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From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [214]
In mice, hepcidin is required for the development of anemia during inflammation.
“In mice, hepcidin was required for the development of anemia during inflammation (214).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [214]
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- Authors
- Müllner, E. W., & Kühn, L. C.
- ORCID
- Kühn, Lukas C.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1988-06
- Volume
- 53
- Issue
- 5
- Pages
- 815-825
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1973592220
- Cited by
- 554
- References
- 51
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 7 · 2017: 9 · 2016: 3 · 2015: 4 · 2014: 9 · 2013: 3 · 2012: 2
- FWCI
- 29.40
- Type
- journal article
- Language
- en
- Open access
- green
- infoscience.epfl.ch
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (3.027-0.84)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- transferrin receptor, 3' untranslated region, stem-loop structure, mRNA stability, post-transcriptional regulation, iron starvation, mRNA half-life, secondary structure, mouse L cells
- MeSH terms
- Base Sequence, Cytoplasm, Gene Expression Regulation, Genes, Humans, Iron, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Receptors, Transferrin, RNA, Messenger, Transferrin
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