Effects of eicosapentaenoic and docosahexaenoic acids on apoprotein B mRNA and secretion of very low-density lipoprotein in HepG2 cells
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Oleic acid (18:1n-9, OA), docosahexaenoic acid (22:6n-3, DHA), or eicosapentaenoic acid (20:5n-3, EPA) was added to HepG2 cells at a concentration of 1 mM in a 5:1 or 2:1 molar complex with bovine serum albumin (BSA), and this was incubated for 3 hours. The incorporation of 3H-glycerol into cellular and medium triglyceride (TG), and the mass of TG were measured. The effects of these fatty acids on the secretion of very low density lipoprotein (VLDL) apolipoprotein B (apo B) were estimated from the incorporation of 3H-leucine into the medium apo B in comparison to cells incubated with fatty acid-poor albumin. The secretion of human albumin by the cells was also estimated by immunochemical precipitation of the labeled albumin. In addition, the intracellular levels of apo B messenger ribonucleic acid (mRNA) were measured by the dot-blot hybridization technique. Relative to control cells incubated with BSA, OA (complexed to BSA at a 5:1 molar ratio) stimulated TG synthesis and secretion sevenfold. Compared to OA, EPA was 24% less effective for both processes, whereas DHA inhibited only the secretion of TG (-43%). The secretion of VLDL apo B was not affected by OA, but was decreased 31% by EPA and 54% by DHA. When the molar ratio of fatty acid complexed to albumin was changed to 2:1, similar results were obtained with respect to TG production. The levels of apo B mRNA relative to actin mRNA were not significantly altered by any of the fatty acids.(ABSTRACT TRUNCATED AT 250 WORDS)
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In human kinetic studies, fish oils slow hepatic VLDL production rate and reduce apoB-100 secretion from HepG2 cells.
“In human kinetic studies, fish oils slow hepatic VLDL production rate (34)(35)(36)(37) and reduce apoB-100 secretion from HepG2 cells (38).”
From Omega-3 fatty acid supplementation accelerates chylomicron triglyceride clearance (2003) · cites this paper as [38]
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- Authors
- Wong, S. H., Fisher, E. A., & Marsh, J. B.
- Affiliations
- Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.
- ORCID
- Fisher, E A
- Venue
- Arteriosclerosis: An Official Journal of the American Heart Association, Inc.
- Published
- 1989-11
- Volume
- 9
- Issue
- 6
- Pages
- 836-841
- ISSN
- 0276-5047 (ISSN-L)
- OpenAlex
- W2006246345
- Cited by
- 91
- References
- 16
- Citations by year
- 2022: 1 · 2019: 2 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 10.62
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Arteriosclerosis An Official Journal of the American Heart Association Inc (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL22633)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- eicosapentaenoic acid, docosahexaenoic acid, oleic acid, very low density lipoprotein secretion, triglyceride synthesis, hepatic lipogenesis, HepG2 cells, omega-3 fatty acids
- MeSH terms
- Albumins, Apolipoproteins B, Docosahexaenoic Acids, Gene Expression Regulation, Humans, Lipoproteins, VLDL, Liver, Oleic Acids, Phospholipids, Triglycerides, Tumor Cells, Cultured, Eicosapentaenoic Acid, Oleic Acid
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