The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Tumor Necrosis Factor-α Promotes In Vitro Calcification of Vascular Cells via the cAMP Pathway
Abstract
Background —Vascular calcification is an ectopic calcification that commonly occurs in atherosclerosis. Because tumor necrosis factor-α (TNF-α), a pleiotropic cytokine found in atherosclerotic lesions, is also a regulator of bone formation, we investigated the role of TNF-α in in vitro vascular calcification. Methods and Results —A cloned subpopulation of bovine aortic smooth muscle cells previously shown capable of osteoblastic differentiation was treated with TNF-α, and osteoblastic differentiation and mineralization were assessed. Treatment of vascular cells with TNF-α for 3 days induced an osteoblast-like morphology. It also enhanced both activity and mRNA expression of alkaline phosphatase, an early marker of osteoblastic differentiation. Continuous treatment with TNF-α for 10 days enhanced matrix mineralization as measured by radiolabeled calcium incorporation in the matrix. Pretreatment of cells with a protein kinase A–specific inhibitor, KT5720, attenuated cell morphology, the alkaline phosphatase activity, and mineralization induced by TNF-α. Consistent with this, the intracellular cAMP level was elevated after TNF-α treatment. Electrophoretic mobility shift assay demonstrated that TNF-α enhanced DNA binding of osteoblast specific factor (Osf2), AP1, and CREB, transcription factors that are important for osteoblastic differentiation. Conclusions —These results suggest that TNF-α enhances in vitro vascular calcification by promoting osteoblastic differentiation of vascular cells through the cAMP pathway.
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Differential Expression of Bone Matrix Regulatory Proteins in Human Atherosclerotic Plaques
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Vascular Smooth Muscle Cell Calcification Is Mediated by Regulated Exosome Secretion
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Metadata
- Authors
- Tintut, Y., Patel, J., Parhami, F., & Demer, L. L.
- Venue
- Circulation
- Published
- 2000-11-21
- Volume
- 102
- Issue
- 21
- Pages
- 2636-2642
- ISSN
- 0009-7322 (ISSN-L)
- 1524-4539
- OpenAlex
- W2170893247
- Cited by
- 527
- References
- 39
- FWCI
- 9.49
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tintut, Y., Patel, J., Parhami, F., & Demer, L. L. (2000). Tumor Necrosis Factor-α Promotes In Vitro Calcification of Vascular Cells via the cAMP Pathway. Circulation. https://doi.org/10.1161/01.cir.102.21.2636
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