The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Shear stress-induced release of nitric oxide from endothelial cells grown on beads.
Abstract
An in vitro bioassay system was developed to study endothelium-mediated, shear stress-induced, or flow-dependent generation of endothelium-derived relaxing factor (EDRF). Monolayers of aortic endothelial cells were grown on a rigid and large surface area of microcarrier beads and were packed in a small column perfused with Krebs bicarbonate solution. The perfusate was allowed to superfuse three endothelium-denuded target pulmonary arterial strips arranged in a cascade. Fluid shear stress caused a flow-dependent release of EDRF from the endothelial cells. The action of EDRF was abolished by oxyhemoglobin and methylene blue, and the generation of EDRF in response to shear stress was markedly inhibited or abolished by NG-nitro-L-arginine, by NG-amino-L-arginine, by calcium-free extracellular medium, and by depleting endothelial cells of endogenous L-arginine. Addition of L-arginine to arginine-deficient but not arginine-containing endothelial cells rapidly restored the capacity of shear stress and bradykinin to generate EDRF. These observations indicate that fluid shear stress causes the generation of EDRF with properties of nitric oxide from aortic endothelial cells and that the bioassay system described may be useful for studying the mechanism of mechanochemical coupling that leads to nitric oxide generation.
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Cites
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Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
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Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide.
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Self-citationVascular endothelial cells synthesize nitric oxide from L-arginine
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Stuehr, D. J., & Nathan, C. F. (1989) · The Journal of Experimental Medicine · doi:10.1084/jem.169.5.1543 · Cited by 1,744
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Self-citationFlow-induced release of endothelium-derived relaxing factor
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Flow Effects on Prostacyclin Production by Cultured Human Endothelial Cells
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Cited by
Flow-mediated endothelial mechanotransduction
Davies, P. F. (1995) · Physiological Reviews · doi:10.1152/physrev.1995.75.3.519 · Cited by 2,802
Chiu, J.-J., & Chien, S. (2011) · Physiological Reviews · doi:10.1152/physrev.00047.2009 · Cited by 2,280
Laminar Shear Stress: Mechanisms by Which Endothelial Cells Transduce an Atheroprotective Force
Traub, O., & Berk, B. C. (1998) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.18.5.677 · Cited by 1,085
Rajfer, J., Aronson, W. J., Bush, P. A., Dorey, F. J., & Ignarro, L. J. (1992) · New England Journal of Medicine · doi:10.1056/nejm199201093260203 · Cited by 1,006
Nitric Oxide Synthesis by Cultured Endothelial Cells Is Modulated by Flow Conditions
Noris, M., Morigi, M., Donadelli, R., Aiello, S., Foppolo, M., Todeschini, M., Orisio, S., Remuzzi, G., & Remuzzi, A. (1995) · Circulation Research · doi:10.1161/01.res.76.4.536 · Cited by 543
Metadata
- Authors
- Buga, G. M., Gold, M. E., Fukuto, J. M., & Ignarro, L. J.
- Venue
- Hypertension
- Published
- 1991-02
- Volume
- 17
- Issue
- 2
- Pages
- 187-193
- ISSN
- 0194-911X (ISSN-L)
- 1524-4563
- OpenAlex
- W2130870274
- Cited by
- 484
- References
- 36
- FWCI
- 14.51
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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