Effect of antioxidant supplementation on ozone-induced lung injury in human subjects
Abstract
To determine whether antioxidants can influence human susceptibility to ozone (O(3))-induced changes in lung function and airway inflammation, we placed 31 healthy nonsmoking adults (18 to 35 yr old) on a diet low in ascorbate for 3 wk. At 1 wk, subjects were exposed to filtered air for 2 h while exercising (20 L/min/m(2)), and then underwent bronchoalveolar lavage (BAL) and were randomly assigned to receive either a placebo or 250 mg of vitamin C, 50 IU of alpha-tocopherol, and 12 oz of vegetable cocktail daily for 2 wk. Subjects were then exposed to 0.4 ppm O(3) for 2 h and underwent a second BAL. On the day of the O(3) exposure, supplemented subjects were found to have significantly increased levels of plasma ascorbate, tocopherols, and carotenoids as compared with those of the placebo group. Pulmonary function testing showed that O(3)-induced reductions in FEV(1) and FVC were 30% and 24% smaller, respectively, in the supplemented cohort. In contrast, the inflammatory response to O(3) inhalation, as represented by the percent neutrophils and the concentration of interleukin-6 recovered in the BAL fluid at 1 h after O(3) exposure was not different for the two groups. These data suggest that dietary antioxidants protect against O(3)-induced pulmonary function decrements in humans.
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In a controlled human exposure study, healthy adults who received dietary antioxidant supplementation exhibited a significantly smaller ozone-induced reduction in pulmonary function.
“In a control human exposure study (2-h exposure to 400 ppb ozone vs. filtered air), healthy adults who had received a dietary antioxidant supplementation of a mixture of vitamin C, alpha-tocopherol, and vegetable cocktail exhibited a significantly smaller ozone-induced reduction in pulmonary function (85).”
From Ozone pollution: A Major health hazard worldwide (2019) · cites this paper as [85]
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- Authors
- SAMET, J. M., HATCH, G. E., HORSTMAN, D., STECK-SCOTT, S., ARAB, L., BROMBERG, P. A., LEVINE, M., MCDONNELL, W. F., & DEVLIN, R. B.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Human Studies and Experimental Toxicology Divisions, National Health and Environmental Effects Research Laboratory, Research Triangle Park, and Department of Nutrition and Center for Environmental Medicine and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; and National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-09-01
- Volume
- 164
- Issue
- 5
- Pages
- 819-825
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- OpenAlex
- W2117497904
- Cited by
- 164
- References
- 47
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 5 · 2021: 2 · 2020: 4 · 2019: 6 · 2018: 1 · 2017: 3 · 2016: 7 · 2015: 9 · 2014: 8 · 2013: 8 · 2012: 7
- FWCI
- 5.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Respiratory and Critical Care Medicine (publishedVersion)
- PubMed (publishedVersion)
- Scholar Commons (University of South Carolina) (submittedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- antioxidant supplementation, ozone exposure, ozone-induced lung injury, vitamin C, alpha-tocopherol, lung function, airway inflammation, bronchoalveolar lavage, neutrophils, interleukin-6, carotenoids, physical activity
- MeSH terms
- Adult, Antioxidants, Female, Humans, Lung Diseases, Male, Ozone
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